Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1556 |
Resumo: | Improved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant flocculating Saccharomyces cerevisiae cells, expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under the ADHI promotor and terminator in a bioreactor was studied. The effects of lactose concentration and yeast extract concentration on β-galactosidase production in a semi-synthetic medium were analysed. The extracellular β-galactosidase activity increased linearly with increasing initial lactose concentrations (5–150 g dmˉ³ ). β-Galactosidase production also increased with increased yeast extract concentration. During the entire fermentation, no accumulation of the hydrolysed sugars, glucose and galactose, was observed. The catabolic repression of the recombinant strain when cultured in a medium containing equal amounts of glucose and galactose was confirmed. In complete anaerobiosis, the fermentation of lactose resulted in a very slow fermentation pattern with lower levels of β-galactosidase activity. The bioreactor operation together with optimisation of culture conditions (lactose and yeast extract concentration) led to a 21-fold increase in the extracellular β-galactosidase activity produced when compared with preliminary Erlenmeyer fermentations. |
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Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactoseβ-galactosidase productionFlocculent yeastExtracellular protein productionHigh-cell-density fermentationbeta-galactosidase productionScience & TechnologyImproved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant flocculating Saccharomyces cerevisiae cells, expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under the ADHI promotor and terminator in a bioreactor was studied. The effects of lactose concentration and yeast extract concentration on β-galactosidase production in a semi-synthetic medium were analysed. The extracellular β-galactosidase activity increased linearly with increasing initial lactose concentrations (5–150 g dmˉ³ ). β-Galactosidase production also increased with increased yeast extract concentration. During the entire fermentation, no accumulation of the hydrolysed sugars, glucose and galactose, was observed. The catabolic repression of the recombinant strain when cultured in a medium containing equal amounts of glucose and galactose was confirmed. In complete anaerobiosis, the fermentation of lactose resulted in a very slow fermentation pattern with lower levels of β-galactosidase activity. The bioreactor operation together with optimisation of culture conditions (lactose and yeast extract concentration) led to a 21-fold increase in the extracellular β-galactosidase activity produced when compared with preliminary Erlenmeyer fermentations.Society of Chemical IndustryUniversidade do MinhoDomingues, LucíliaOliveira, Carla Cristina Marques deCastro, InêsLima, NelsonTeixeira, J. A.20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1556eng“Journal of chemical technology and biotechnology”. ISSN 0268-2575. 79 (2004) 809-815.0268-257510.1002/jctb.1025info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:52Zoai:repositorium.sdum.uminho.pt:1822/1556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:27.779796Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
title |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
spellingShingle |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose Domingues, Lucília β-galactosidase production Flocculent yeast Extracellular protein production High-cell-density fermentation beta-galactosidase production Science & Technology |
title_short |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
title_full |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
title_fullStr |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
title_full_unstemmed |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
title_sort |
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose |
author |
Domingues, Lucília |
author_facet |
Domingues, Lucília Oliveira, Carla Cristina Marques de Castro, Inês Lima, Nelson Teixeira, J. A. |
author_role |
author |
author2 |
Oliveira, Carla Cristina Marques de Castro, Inês Lima, Nelson Teixeira, J. A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Domingues, Lucília Oliveira, Carla Cristina Marques de Castro, Inês Lima, Nelson Teixeira, J. A. |
dc.subject.por.fl_str_mv |
β-galactosidase production Flocculent yeast Extracellular protein production High-cell-density fermentation beta-galactosidase production Science & Technology |
topic |
β-galactosidase production Flocculent yeast Extracellular protein production High-cell-density fermentation beta-galactosidase production Science & Technology |
description |
Improved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant flocculating Saccharomyces cerevisiae cells, expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under the ADHI promotor and terminator in a bioreactor was studied. The effects of lactose concentration and yeast extract concentration on β-galactosidase production in a semi-synthetic medium were analysed. The extracellular β-galactosidase activity increased linearly with increasing initial lactose concentrations (5–150 g dmˉ³ ). β-Galactosidase production also increased with increased yeast extract concentration. During the entire fermentation, no accumulation of the hydrolysed sugars, glucose and galactose, was observed. The catabolic repression of the recombinant strain when cultured in a medium containing equal amounts of glucose and galactose was confirmed. In complete anaerobiosis, the fermentation of lactose resulted in a very slow fermentation pattern with lower levels of β-galactosidase activity. The bioreactor operation together with optimisation of culture conditions (lactose and yeast extract concentration) led to a 21-fold increase in the extracellular β-galactosidase activity produced when compared with preliminary Erlenmeyer fermentations. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1556 |
url |
http://hdl.handle.net/1822/1556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
“Journal of chemical technology and biotechnology”. ISSN 0268-2575. 79 (2004) 809-815. 0268-2575 10.1002/jctb.1025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society of Chemical Industry |
publisher.none.fl_str_mv |
Society of Chemical Industry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132613360746496 |