Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/50197 |
Resumo: | Pawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in Rats |
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Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in ratsmotor preferencelateralityhandednesscognitionimpulsivitymemorybehaviorScience & TechnologyPawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in RatsIn rodents, dexterity is commonly analyzed in preference paradigms in which animals are given the chance to use either the left or the right front paws to manipulate food. However, paw preference and dexterity at population and individual levels are controversial as results are incongruent across paradigms. We have therefore developed a semi-quantitative method-the pawdeness trait test (PaTRaT)-to evaluate paw preference degree in rats. The PaTRaT consists in a classification system, ranging from +4 to 4 where increasingly positive and negative values reflect the bias for left or right paw use, respectively. Sprague-Dawley male rats were confined into a metal rectangular mesh cylinder, from which they can see, smell and reach sugared rewards with their paws. Due to its size, the reward could only cross the mesh if aligned with its diagonal, imposing additional coordination. Animals were allowed to retrieve 10 rewards per session in a total of four sessions while their behavior was recorded. PaTRaT was repeated 4 and 8 weeks after the first evaluation. To exclude potential bias, rats were also tested for paw fine movement and general locomotion in other behavioral paradigms as well as impulsivity (variable delay-to-signal, VDS), memory and cognitive flexibility (water maze). At the population level 54% of the animals presented a rightward bias. Individually, all animals presented marked side-preferences, >2 and <-2 for left-and right-sided bias, respectively, and this preference was stable across the three evaluations. Inter-rater consistency was very high between two experienced raters and substantial when two additional inexperienced raters were included. Left-and right-biased animals presented no differences in the ability to perform fine movements with any of the forelimbs (staircase) and general locomotor performance. Additionally, these groups performed similarly in executive function and memory tasks. In conclusion, PaTRaT is able to reliably classify rats' pawedness direction and degree.This work has been funded by the European Regional Development Fund (FEDER), through the Competitiveness Factors Operational Programme (COMPETE) and the Northern Portugal Regional Operational Programme (NORTE 2020) under the Portugal 2020 Partnership Agreement (project NORTE-01-0145-FEDER-000023). It was also funded by National funds, through the Foundation for Science and Technology (Fundacao para a Ciencia e a Tecnologia, FCT), under the scope of the projects POCI-01-0145-FEDER-007038 and PTDC/NEU-SCC/5301/2014. Researchers were supported by FCT grant numbers SFRH/BD/109111/2015 (AMC), SFRH/BD/52291/2013 (ME via Inter-University Doctoral Programme in Ageing and Chronic Disease, PhDOC), PD/BD/114120/2015 (SPN via PhDOC), SFRH/BD/89936/2012 (SB), PD/BD/114117/2015 (MRG via PhDOC) and SFRH/BPD/80118/2011 (HL-A).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoCunha, Ana M.Esteves, Madalena CurvaNeves, Sofia PereiraBorges, Sónia Maria de SousaGuimarães, Marco Rafael MachadoSousa, NunoAlmeida, ArmandoLeite-Almeida, Hugo20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/50197eng1662-51531662-515310.3389/fnbeh.2017.00192https://www.frontiersin.orginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:51Zoai:repositorium.sdum.uminho.pt:1822/50197Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:40.412493Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| title |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| spellingShingle |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats Cunha, Ana M. motor preference laterality handedness cognition impulsivity memory behavior Science & Technology |
| title_short |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| title_full |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| title_fullStr |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| title_full_unstemmed |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| title_sort |
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats |
| author |
Cunha, Ana M. |
| author_facet |
Cunha, Ana M. Esteves, Madalena Curva Neves, Sofia Pereira Borges, Sónia Maria de Sousa Guimarães, Marco Rafael Machado Sousa, Nuno Almeida, Armando Leite-Almeida, Hugo |
| author_role |
author |
| author2 |
Esteves, Madalena Curva Neves, Sofia Pereira Borges, Sónia Maria de Sousa Guimarães, Marco Rafael Machado Sousa, Nuno Almeida, Armando Leite-Almeida, Hugo |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Cunha, Ana M. Esteves, Madalena Curva Neves, Sofia Pereira Borges, Sónia Maria de Sousa Guimarães, Marco Rafael Machado Sousa, Nuno Almeida, Armando Leite-Almeida, Hugo |
| dc.subject.por.fl_str_mv |
motor preference laterality handedness cognition impulsivity memory behavior Science & Technology |
| topic |
motor preference laterality handedness cognition impulsivity memory behavior Science & Technology |
| description |
Pawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in Rats |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/50197 |
| url |
http://hdl.handle.net/1822/50197 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1662-5153 1662-5153 10.3389/fnbeh.2017.00192 https://www.frontiersin.org |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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