Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/36582 |
Resumo: | PMID: 26664012 WOS: 000362879000003 |
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Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicusJournal ArticleSDG 3 - Good Health and Well-beingPMID: 26664012 WOS: 000362879000003BACKGROUND: Ampelozizyphus amazonicus Ducke, a plant that is widely used by the population of the Amazonian region to prevent and treat malaria, was investigated in this work, which describes, for the first time, the antiplasmodial activity of its extracts and associates this activity with its isolated constituents. METHODS: Different extracts with solvents of increasing polarity (hexane, chloroform, ethanol, and water) were obtained of the root bark. This procedure resulted in extracts that were characterized for their constituents. The cytotoxicity and activity of the extracts against Plasmodium berghei (schizontocidal activity, liver stage) and Plasmodium falciparum (3D7 and Dd2 strains, erythrocyte stage) were assessed in vitro. RESULTS: Of the four extracts assayed against P. berghei, the chloroform extract showed the greatest activity, with an inhibitory concentration 50% (IC50) value of 30.1 µg/mL, followed by the aqueous extract (IC50 = 39.9 µg/mL). The chloroform extract exhibited the highest antiplasmodial activity in the erythrocyte stage of P. falciparum, with an IC50 value lower than 15 µg/mL. Fractionation of this more active extract led to the isolation and elucidation of pentacyclic triterpenes, lupeol, betulin and betulinic acid, which showed antiplasmodial activities with IC50 values ranging from 5.6 to 80.30 µM. The most active of these, betulinic acid, was further quantified in the extracts by high-performance liquid chromatography-photodiode array detector analyzes. The higher amount was found in the chloroform extract, which was the most active one against P. falciparum. CONCLUSION: The results obtained in this work may partly explain the popular intake of A. amazonicusas an antimalarial remedy in the Amazon region.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNdo Carmo, Dominique F MAmaral, Ana Claudia FMachado, MartaLopes, DinoraEchevarria, AureaRosário, Virgílio ESilva, Jefferson Rocha de A2018-05-11T22:02:30Z2015-102015-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/36582eng0973-1296PURE: 1662778https://doi.org/10.4103/0973-1296.166071info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:32:35Zoai:run.unl.pt:10362/36582Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:32:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| title |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| spellingShingle |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus do Carmo, Dominique F M Journal Article SDG 3 - Good Health and Well-being |
| title_short |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| title_full |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| title_fullStr |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| title_full_unstemmed |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| title_sort |
Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus |
| author |
do Carmo, Dominique F M |
| author_facet |
do Carmo, Dominique F M Amaral, Ana Claudia F Machado, Marta Lopes, Dinora Echevarria, Aurea Rosário, Virgílio E Silva, Jefferson Rocha de A |
| author_role |
author |
| author2 |
Amaral, Ana Claudia F Machado, Marta Lopes, Dinora Echevarria, Aurea Rosário, Virgílio E Silva, Jefferson Rocha de A |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
do Carmo, Dominique F M Amaral, Ana Claudia F Machado, Marta Lopes, Dinora Echevarria, Aurea Rosário, Virgílio E Silva, Jefferson Rocha de A |
| dc.subject.por.fl_str_mv |
Journal Article SDG 3 - Good Health and Well-being |
| topic |
Journal Article SDG 3 - Good Health and Well-being |
| description |
PMID: 26664012 WOS: 000362879000003 |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-10 2015-10-01T00:00:00Z 2018-05-11T22:02:30Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/36582 |
| url |
http://hdl.handle.net/10362/36582 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0973-1296 PURE: 1662778 https://doi.org/10.4103/0973-1296.166071 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545637943574528 |