Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Detalhes bibliográficos
Autor(a) principal: Alves, Rita C.
Data de Publicação: 2015
Outros Autores: Pimentel, Filipa B., Nouws, Henri P. A., Correr, Wagner, González-García, María Begoña, Oliveira, M. Beatriz P. P., Delerue-Matos, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/7224
Resumo: A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
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spelling Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approachElectrochemical immunosensorAra h 6VoltammetryScreen-printed carbon electrodesAllergenPeanutA voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.SpringerRepositório Científico do Instituto Politécnico do PortoAlves, Rita C.Pimentel, Filipa B.Nouws, Henri P. A.Correr, WagnerGonzález-García, María BegoñaOliveira, M. Beatriz P. P.Delerue-Matos, Cristina2015-12-22T16:09:24Z2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/7224eng1618-265010.1007/s00216-015-8879-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:47:43Zoai:recipp.ipp.pt:10400.22/7224Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:46.915647Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
title Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
spellingShingle Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
Alves, Rita C.
Electrochemical immunosensor
Ara h 6
Voltammetry
Screen-printed carbon electrodes
Allergen
Peanut
title_short Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
title_full Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
title_fullStr Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
title_full_unstemmed Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
title_sort Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
author Alves, Rita C.
author_facet Alves, Rita C.
Pimentel, Filipa B.
Nouws, Henri P. A.
Correr, Wagner
González-García, María Begoña
Oliveira, M. Beatriz P. P.
Delerue-Matos, Cristina
author_role author
author2 Pimentel, Filipa B.
Nouws, Henri P. A.
Correr, Wagner
González-García, María Begoña
Oliveira, M. Beatriz P. P.
Delerue-Matos, Cristina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Alves, Rita C.
Pimentel, Filipa B.
Nouws, Henri P. A.
Correr, Wagner
González-García, María Begoña
Oliveira, M. Beatriz P. P.
Delerue-Matos, Cristina
dc.subject.por.fl_str_mv Electrochemical immunosensor
Ara h 6
Voltammetry
Screen-printed carbon electrodes
Allergen
Peanut
topic Electrochemical immunosensor
Ara h 6
Voltammetry
Screen-printed carbon electrodes
Allergen
Peanut
description A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-22T16:09:24Z
2015-07
2015-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/7224
url http://hdl.handle.net/10400.22/7224
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1618-2650
10.1007/s00216-015-8879-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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