Molecular characterization of diaporthe species associated with hazelnut defects
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/71278 |
Resumo: | Fungi of the genus Diaporthe have been reported as the main causative agent of hazelnut defects in the Caucasus area. This study aimed to define which fungal species are present in defective hazelnuts grown in Turkey and confirm the role of Diaporthe spp. Seven hazelnut orchards were selected, with each one located in a different Turkish Province (Düzce, Giresun, Ordu, Samsun, Sakarya, Trabzon, and Zonguldak), and hazelnuts were collected at early and full ripening. Fungal isolation and identification were performed at the genus level based on morphological characteristics. Several genera were isolated, with Diaporthe spp. being among the prevalent. This was the only genus with increasing incidence from early to full ripening, and incidence at full ripening was positively correlated both with internal (?=0.86) and visible defects (?=0.81), which confirmed its role as the key causative agent of hazelnut defects. The correlation of defect occurrence with rainfall, reported in previous study, was not confirmed, possibly due to the low defect incidence. A total of 86 Diaporthe monosporic strains isolated from Turkish hazelnut samples, together with 33 strains collected in the Caucasus region and 6 from Italy, were analyzed with a multi-locus phylogeny based on three genomic loci (ITS, EF1-?, and tub). The results showed that Diaporthe strains can be grouped into 7 distinct clades, with a majority of Turkish strains (95%) being placed into a single clade related with D. eres. These samples were organized into several sub-clades, which indicates the existence of genetically diverse sub-populations. |
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Molecular characterization of diaporthe species associated with hazelnut defectsfungiDiaporthePhomopsishazelnutrottenmolecular phylogenymulti-locus sequence analysisTurkeyScience & TechnologyFungi of the genus Diaporthe have been reported as the main causative agent of hazelnut defects in the Caucasus area. This study aimed to define which fungal species are present in defective hazelnuts grown in Turkey and confirm the role of Diaporthe spp. Seven hazelnut orchards were selected, with each one located in a different Turkish Province (Düzce, Giresun, Ordu, Samsun, Sakarya, Trabzon, and Zonguldak), and hazelnuts were collected at early and full ripening. Fungal isolation and identification were performed at the genus level based on morphological characteristics. Several genera were isolated, with Diaporthe spp. being among the prevalent. This was the only genus with increasing incidence from early to full ripening, and incidence at full ripening was positively correlated both with internal (?=0.86) and visible defects (?=0.81), which confirmed its role as the key causative agent of hazelnut defects. The correlation of defect occurrence with rainfall, reported in previous study, was not confirmed, possibly due to the low defect incidence. A total of 86 Diaporthe monosporic strains isolated from Turkish hazelnut samples, together with 33 strains collected in the Caucasus region and 6 from Italy, were analyzed with a multi-locus phylogeny based on three genomic loci (ITS, EF1-?, and tub). The results showed that Diaporthe strains can be grouped into 7 distinct clades, with a majority of Turkish strains (95%) being placed into a single clade related with D. eres. These samples were organized into several sub-clades, which indicates the existence of genetically diverse sub-populations.This research was partially funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoArciuolo, RobertaSantos, Carla Isabel ArcanjoSoares, Célia Maria GonçalvesCastello, GiuseppeSpigolon, NicolaChiusa, GiorgioLima, NelsonBattilani, Paola20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/71278engArciuolo, Roberta; Santos, Carla; Soares, Célia; Castello, Giuseppe; Spigolon, Nicola; Chiusa, Giorgio; Lima, Nelson; Battilani, Paola, Molecular characterization of diaporthe species associated with hazelnut defects. Frontiers in Plant Science, 11(611655), 20201664-462X10.3389/fpls.2020.611655http://www.frontiersin.org/Plant_Physiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:46Zoai:repositorium.sdum.uminho.pt:1822/71278Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:05.014182Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular characterization of diaporthe species associated with hazelnut defects |
title |
Molecular characterization of diaporthe species associated with hazelnut defects |
spellingShingle |
Molecular characterization of diaporthe species associated with hazelnut defects Arciuolo, Roberta fungi Diaporthe Phomopsis hazelnut rotten molecular phylogeny multi-locus sequence analysis Turkey Science & Technology |
title_short |
Molecular characterization of diaporthe species associated with hazelnut defects |
title_full |
Molecular characterization of diaporthe species associated with hazelnut defects |
title_fullStr |
Molecular characterization of diaporthe species associated with hazelnut defects |
title_full_unstemmed |
Molecular characterization of diaporthe species associated with hazelnut defects |
title_sort |
Molecular characterization of diaporthe species associated with hazelnut defects |
author |
Arciuolo, Roberta |
author_facet |
Arciuolo, Roberta Santos, Carla Isabel Arcanjo Soares, Célia Maria Gonçalves Castello, Giuseppe Spigolon, Nicola Chiusa, Giorgio Lima, Nelson Battilani, Paola |
author_role |
author |
author2 |
Santos, Carla Isabel Arcanjo Soares, Célia Maria Gonçalves Castello, Giuseppe Spigolon, Nicola Chiusa, Giorgio Lima, Nelson Battilani, Paola |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Arciuolo, Roberta Santos, Carla Isabel Arcanjo Soares, Célia Maria Gonçalves Castello, Giuseppe Spigolon, Nicola Chiusa, Giorgio Lima, Nelson Battilani, Paola |
dc.subject.por.fl_str_mv |
fungi Diaporthe Phomopsis hazelnut rotten molecular phylogeny multi-locus sequence analysis Turkey Science & Technology |
topic |
fungi Diaporthe Phomopsis hazelnut rotten molecular phylogeny multi-locus sequence analysis Turkey Science & Technology |
description |
Fungi of the genus Diaporthe have been reported as the main causative agent of hazelnut defects in the Caucasus area. This study aimed to define which fungal species are present in defective hazelnuts grown in Turkey and confirm the role of Diaporthe spp. Seven hazelnut orchards were selected, with each one located in a different Turkish Province (Düzce, Giresun, Ordu, Samsun, Sakarya, Trabzon, and Zonguldak), and hazelnuts were collected at early and full ripening. Fungal isolation and identification were performed at the genus level based on morphological characteristics. Several genera were isolated, with Diaporthe spp. being among the prevalent. This was the only genus with increasing incidence from early to full ripening, and incidence at full ripening was positively correlated both with internal (?=0.86) and visible defects (?=0.81), which confirmed its role as the key causative agent of hazelnut defects. The correlation of defect occurrence with rainfall, reported in previous study, was not confirmed, possibly due to the low defect incidence. A total of 86 Diaporthe monosporic strains isolated from Turkish hazelnut samples, together with 33 strains collected in the Caucasus region and 6 from Italy, were analyzed with a multi-locus phylogeny based on three genomic loci (ITS, EF1-?, and tub). The results showed that Diaporthe strains can be grouped into 7 distinct clades, with a majority of Turkish strains (95%) being placed into a single clade related with D. eres. These samples were organized into several sub-clades, which indicates the existence of genetically diverse sub-populations. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/71278 |
url |
http://hdl.handle.net/1822/71278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arciuolo, Roberta; Santos, Carla; Soares, Célia; Castello, Giuseppe; Spigolon, Nicola; Chiusa, Giorgio; Lima, Nelson; Battilani, Paola, Molecular characterization of diaporthe species associated with hazelnut defects. Frontiers in Plant Science, 11(611655), 2020 1664-462X 10.3389/fpls.2020.611655 http://www.frontiersin.org/Plant_Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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