Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics

Detalhes bibliográficos
Autor(a) principal: Machado, Ana
Data de Publicação: 2017
Outros Autores: Mesquita, Raquel B. R., Mesquita, Letícia S., Rangel, Maria, Rangel, António O. S. S., Bordalo, Adriano A.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/41470
Resumo: Background Iron has a crucial role in plant nutrition, being an essential element for plant growth. However, one-third of the Earth soil is iron deficient, resulting in iron deficiency chlorosis (IDC) growth of several crops worldwide, including staple foods. The use of synthetic Fe(III)-chelates is one of the most effective measures to correct IDC in plants, but their environmental impact must be mastered. Therefore, the search for more effective Fe-chelates remains an important issue. Previously [1], a 3,4-HPO Fe-chelate was proposed as a novel-fertilizing agent. Since the increase of nutrients availability in soil is thought to have an impact on the microbial composition, this question needs to be addressed. Therefore, the aim of the study was to investigate the effect of iron complexes of the 3,4-HPO class of ligands on soil bacterial dynamics to better understand their pathways. Method Laboratory scale soil columns (LSSC) were set up, with different soils origin and characteristics (Agricultural, Forestry and Urban), and exposed to two iron-chelates using rain simulations. The structure and abundance of the bacterial community was evaluated by automated ribosomal intergenic spacer analysis (ARISA) and qPCR (rpoB) approaches. Results & Conclusions Cluster analysis of ARISA profiles revealed that the soil characteristics were the major driving selection for the microbial community composition, with the samples from the same soil type clustering together, disregarding the amendment performed. Also, it emerged that the microbial community of forestry and agricultural soils were more similar (46%) than the one present in urban soil, as expected due to the plant influence. Considering each soil type individually, it was possible to observe a clear response to Fe(III) amendment on the microbial assemblage. Additionally, it seems that one of the compounds, the Fe-chelate derived from the ligand Deferiprone, induced greater and/or faster changes, and that the forestry soil was more prone to the microbial shift. These changes can underline a selection for bacteria that can use Fe(III) in its metabolism, or more tolerant to its presence, that needs to be better understood.
id RCAP_ab398ce8e945a27be602c031d28aaef2
oai_identifier_str oai:repositorio.ucp.pt:10400.14/41470
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamicsSoilIron-chelatesMicrobial diversityBackground Iron has a crucial role in plant nutrition, being an essential element for plant growth. However, one-third of the Earth soil is iron deficient, resulting in iron deficiency chlorosis (IDC) growth of several crops worldwide, including staple foods. The use of synthetic Fe(III)-chelates is one of the most effective measures to correct IDC in plants, but their environmental impact must be mastered. Therefore, the search for more effective Fe-chelates remains an important issue. Previously [1], a 3,4-HPO Fe-chelate was proposed as a novel-fertilizing agent. Since the increase of nutrients availability in soil is thought to have an impact on the microbial composition, this question needs to be addressed. Therefore, the aim of the study was to investigate the effect of iron complexes of the 3,4-HPO class of ligands on soil bacterial dynamics to better understand their pathways. Method Laboratory scale soil columns (LSSC) were set up, with different soils origin and characteristics (Agricultural, Forestry and Urban), and exposed to two iron-chelates using rain simulations. The structure and abundance of the bacterial community was evaluated by automated ribosomal intergenic spacer analysis (ARISA) and qPCR (rpoB) approaches. Results & Conclusions Cluster analysis of ARISA profiles revealed that the soil characteristics were the major driving selection for the microbial community composition, with the samples from the same soil type clustering together, disregarding the amendment performed. Also, it emerged that the microbial community of forestry and agricultural soils were more similar (46%) than the one present in urban soil, as expected due to the plant influence. Considering each soil type individually, it was possible to observe a clear response to Fe(III) amendment on the microbial assemblage. Additionally, it seems that one of the compounds, the Fe-chelate derived from the ligand Deferiprone, induced greater and/or faster changes, and that the forestry soil was more prone to the microbial shift. These changes can underline a selection for bacteria that can use Fe(III) in its metabolism, or more tolerant to its presence, that needs to be better understood.Veritati - Repositório Institucional da Universidade Católica PortuguesaMachado, AnaMesquita, Raquel B. R.Mesquita, Letícia S.Rangel, MariaRangel, António O. S. S.Bordalo, Adriano A.2023-06-28T15:29:19Z2017-122017-12-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10400.14/41470enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:42:33Zoai:repositorio.ucp.pt:10400.14/41470Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:42:33Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
title Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
spellingShingle Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
Machado, Ana
Soil
Iron-chelates
Microbial diversity
title_short Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
title_full Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
title_fullStr Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
title_full_unstemmed Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
title_sort Assessment of the impact synthetic Fe(III)-chelates amendment in soil microbial community dynamics
author Machado, Ana
author_facet Machado, Ana
Mesquita, Raquel B. R.
Mesquita, Letícia S.
Rangel, Maria
Rangel, António O. S. S.
Bordalo, Adriano A.
author_role author
author2 Mesquita, Raquel B. R.
Mesquita, Letícia S.
Rangel, Maria
Rangel, António O. S. S.
Bordalo, Adriano A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Machado, Ana
Mesquita, Raquel B. R.
Mesquita, Letícia S.
Rangel, Maria
Rangel, António O. S. S.
Bordalo, Adriano A.
dc.subject.por.fl_str_mv Soil
Iron-chelates
Microbial diversity
topic Soil
Iron-chelates
Microbial diversity
description Background Iron has a crucial role in plant nutrition, being an essential element for plant growth. However, one-third of the Earth soil is iron deficient, resulting in iron deficiency chlorosis (IDC) growth of several crops worldwide, including staple foods. The use of synthetic Fe(III)-chelates is one of the most effective measures to correct IDC in plants, but their environmental impact must be mastered. Therefore, the search for more effective Fe-chelates remains an important issue. Previously [1], a 3,4-HPO Fe-chelate was proposed as a novel-fertilizing agent. Since the increase of nutrients availability in soil is thought to have an impact on the microbial composition, this question needs to be addressed. Therefore, the aim of the study was to investigate the effect of iron complexes of the 3,4-HPO class of ligands on soil bacterial dynamics to better understand their pathways. Method Laboratory scale soil columns (LSSC) were set up, with different soils origin and characteristics (Agricultural, Forestry and Urban), and exposed to two iron-chelates using rain simulations. The structure and abundance of the bacterial community was evaluated by automated ribosomal intergenic spacer analysis (ARISA) and qPCR (rpoB) approaches. Results & Conclusions Cluster analysis of ARISA profiles revealed that the soil characteristics were the major driving selection for the microbial community composition, with the samples from the same soil type clustering together, disregarding the amendment performed. Also, it emerged that the microbial community of forestry and agricultural soils were more similar (46%) than the one present in urban soil, as expected due to the plant influence. Considering each soil type individually, it was possible to observe a clear response to Fe(III) amendment on the microbial assemblage. Additionally, it seems that one of the compounds, the Fe-chelate derived from the ligand Deferiprone, induced greater and/or faster changes, and that the forestry soil was more prone to the microbial shift. These changes can underline a selection for bacteria that can use Fe(III) in its metabolism, or more tolerant to its presence, that needs to be better understood.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2017-12-01T00:00:00Z
2023-06-28T15:29:19Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/41470
url http://hdl.handle.net/10400.14/41470
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817547089633083392