Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus

Detalhes bibliográficos
Autor(a) principal: Frazao, Margarida Archer
Data de Publicação: 2010
Outros Autores: Santos, Maria Helena, Brito, J.A., Borges, N.
Tipo de documento: Artigo
Idioma: und
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/94026
Resumo: Brito, J. A.
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spelling Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidusMYO-INOSITOL-PHOSPHATEBrito, J. A.Archaeoglobus fulgidus, a hyperthermophilic archaeon, accumulates di-myo-inositol phosphate (DIP) in response to heat stress. Recently, the pathway for biosynthesis of DIP has been elucidated in this organism and involves a bifunctional enzyme that contains two domains: CTP:inositol-1-phosphate cytidylyltransferase (IPCT) as a soluble domain and di-myo-inositol-1,3'-phosphate-1-phosphate synthase (DIPPS) as a membrane domain. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of the IPCT domain from A. fulgidus in the apo form are reported. The crystals diffracted to 2.4 A resolution using a synchrotron source and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 154.7, b = 83.9, c = 127.7 A.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNFrazao, Margarida ArcherSantos, Maria HelenaBrito, J.A.Borges, N.2020-03-09T23:15:33Z2010-01-012010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/94026und1744-3091PURE: 452563https://doi.org/10.1107/s1744309110032677info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:42:09Zoai:run.unl.pt:10362/94026Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:53.095361Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
title Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
spellingShingle Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
Frazao, Margarida Archer
MYO-INOSITOL-PHOSPHATE
title_short Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
title_full Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
title_fullStr Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
title_full_unstemmed Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
title_sort Production, crystallization and preliminary X-ray analysis of CTP: inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
author Frazao, Margarida Archer
author_facet Frazao, Margarida Archer
Santos, Maria Helena
Brito, J.A.
Borges, N.
author_role author
author2 Santos, Maria Helena
Brito, J.A.
Borges, N.
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Frazao, Margarida Archer
Santos, Maria Helena
Brito, J.A.
Borges, N.
dc.subject.por.fl_str_mv MYO-INOSITOL-PHOSPHATE
topic MYO-INOSITOL-PHOSPHATE
description Brito, J. A.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2010-01-01T00:00:00Z
2020-03-09T23:15:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/94026
url http://hdl.handle.net/10362/94026
dc.language.iso.fl_str_mv und
language und
dc.relation.none.fl_str_mv 1744-3091
PURE: 452563
https://doi.org/10.1107/s1744309110032677
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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