Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal

Detalhes bibliográficos
Autor(a) principal: Leão, Célia
Data de Publicação: 2021
Outros Autores: Clemente, Maria, Moura, Laura, Seyfarth, Anne Mette, Hansen, Inge M., Hendriksen, Rene S., Amaro, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22504
Resumo: Research Areas: Microbiology
id RCAP_aca7ebd068b30e4dac794f36c97bccd2
oai_identifier_str oai:www.repository.utl.pt:10400.5/22504
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in PortugalEscherichia coliretail meatESBLWGSCTX-M-65chromosomeResearch Areas: MicrobiologyThe emergence and dissemination of resistance to third- and fourth-generation cephalosporins among Enterobacteriaceae from different sources impose a global public health threat. Here, we characterized by whole-genome sequencing four Escherichia coli strains harboring the blaCTX−M−65 gene identified among 49 isolates from beef and pork collected at retail. The genomic content was determined using the Center for Genomic Epidemiology web tools. Additionally, the prediction and reconstruction of plasmids were conducted, the genetic platform of the blaCTX−M−65 genes was investigated, and phylogenetic analysis was carried out using 17 other genomes with the same sequence type and harboring the blaCTX−M−65 gene. All strains harbored blaCTX−M−65, blaOXA−1, and blaTEM−1B, and one also carried the blaSHV−12 gene. Other resistance genes, namely, qnrS2, aac(60 )-Ib-c, dfrA14, sul2, tetA, and mphA, were present in all the genomes; the mcr-1.1 gene was identified in the colistinresistant strains. They belong to sequence type 2179, phylogenetic group B1, and serotype O9:H9 and carried plasmids IncI, IncFIC(FII), and IncFIB. All strains share an identical genetic environment with IS903 and ISEcp1 flanking the blaCTX−M−65 gene. It seems likely that the blaCTX−M−65 gene is located in the chromosome in all isolates based on deep in silico analysis. Our findings showed that the strains are clonally related and belong to two sub-lineages. This study reports the emergence of CTX-M-65- producing E. coli in Portugal in food products of animal origin. The chromosomal location of the blaCTX−M−65 gene may ensure a stable spread of resistance in the absence of selective pressure.FRONTIERS MEDIA SARepositório da Universidade de LisboaLeão, CéliaClemente, MariaMoura, LauraSeyfarth, Anne MetteHansen, Inge M.Hendriksen, Rene S.Amaro, Ana2021-11-04T17:45:39Z2021-07-202021-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22504engLeão C, Clemente L, Moura L, Seyfarth AM, Hansen IM, Hendriksen RS, Amaro A. 2021. Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal. Frontiers in Microbiology 12:653595. DOI: 10.3389/fmicb.2021.65359510.3389/fmicb.2021.6535951664-302Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:01Zoai:www.repository.utl.pt:10400.5/22504Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:54.893945Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
title Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
spellingShingle Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
Leão, Célia
Escherichia coli
retail meat
ESBL
WGS
CTX-M-65
chromosome
title_short Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
title_full Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
title_fullStr Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
title_full_unstemmed Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
title_sort Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal
author Leão, Célia
author_facet Leão, Célia
Clemente, Maria
Moura, Laura
Seyfarth, Anne Mette
Hansen, Inge M.
Hendriksen, Rene S.
Amaro, Ana
author_role author
author2 Clemente, Maria
Moura, Laura
Seyfarth, Anne Mette
Hansen, Inge M.
Hendriksen, Rene S.
Amaro, Ana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Leão, Célia
Clemente, Maria
Moura, Laura
Seyfarth, Anne Mette
Hansen, Inge M.
Hendriksen, Rene S.
Amaro, Ana
dc.subject.por.fl_str_mv Escherichia coli
retail meat
ESBL
WGS
CTX-M-65
chromosome
topic Escherichia coli
retail meat
ESBL
WGS
CTX-M-65
chromosome
description Research Areas: Microbiology
publishDate 2021
dc.date.none.fl_str_mv 2021-11-04T17:45:39Z
2021-07-20
2021-07-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22504
url http://hdl.handle.net/10400.5/22504
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Leão C, Clemente L, Moura L, Seyfarth AM, Hansen IM, Hendriksen RS, Amaro A. 2021. Emergence and clonal spread of CTX-M-65-Producing Escherichia coli from retail meat in Portugal. Frontiers in Microbiology 12:653595. DOI: 10.3389/fmicb.2021.653595
10.3389/fmicb.2021.653595
1664-302X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv FRONTIERS MEDIA SA
publisher.none.fl_str_mv FRONTIERS MEDIA SA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131162086473728

Registros relacionados