Structure of microbial communities when complementary effluents are anaerobically digested
Main Author: | |
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Publication Date: | 2021 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.9/3592 |
Summary: | ABSTRACT: Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents-piggery effluent and olive mill wastewater-through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples. |
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Structure of microbial communities when complementary effluents are anaerobically digestedBiogas productionAnaerobic digestionOrganic wastePiggery effluentOlive mill wastewaterWastewater treatmentABSTRACT: Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents-piggery effluent and olive mill wastewater-through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.MDPIRepositório do LNEGEusebio, AnaNeves, AndréMarques, Isabel Paula2021-10-22T11:22:49Z2021-022021-05-19T10:37:19Z2021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3592engEusébio, Ana... [et.al.] - Structure of microbial communities when complementary effluents are anaerobically digested. In: Applied Sciences, 2021, Vol. 11 (3), article nº 1293cv-prod-214329510.3390/app110312932076-3417info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-02-12T05:16:24Zoai:repositorio.lneg.pt:10400.9/3592Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:36:46.477233Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structure of microbial communities when complementary effluents are anaerobically digested |
title |
Structure of microbial communities when complementary effluents are anaerobically digested |
spellingShingle |
Structure of microbial communities when complementary effluents are anaerobically digested Eusebio, Ana Biogas production Anaerobic digestion Organic waste Piggery effluent Olive mill wastewater Wastewater treatment |
title_short |
Structure of microbial communities when complementary effluents are anaerobically digested |
title_full |
Structure of microbial communities when complementary effluents are anaerobically digested |
title_fullStr |
Structure of microbial communities when complementary effluents are anaerobically digested |
title_full_unstemmed |
Structure of microbial communities when complementary effluents are anaerobically digested |
title_sort |
Structure of microbial communities when complementary effluents are anaerobically digested |
author |
Eusebio, Ana |
author_facet |
Eusebio, Ana Neves, André Marques, Isabel Paula |
author_role |
author |
author2 |
Neves, André Marques, Isabel Paula |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Eusebio, Ana Neves, André Marques, Isabel Paula |
dc.subject.por.fl_str_mv |
Biogas production Anaerobic digestion Organic waste Piggery effluent Olive mill wastewater Wastewater treatment |
topic |
Biogas production Anaerobic digestion Organic waste Piggery effluent Olive mill wastewater Wastewater treatment |
description |
ABSTRACT: Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents-piggery effluent and olive mill wastewater-through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-22T11:22:49Z 2021-02 2021-05-19T10:37:19Z 2021-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/3592 |
url |
http://hdl.handle.net/10400.9/3592 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eusébio, Ana... [et.al.] - Structure of microbial communities when complementary effluents are anaerobically digested. In: Applied Sciences, 2021, Vol. 11 (3), article nº 1293 cv-prod-2143295 10.3390/app11031293 2076-3417 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130234833862656 |