Evaluation of GSH adducts of adrenaline in biological samples

Detalhes bibliográficos
Autor(a) principal: Silva, Renata
Data de Publicação: 2007
Outros Autores: Boldt, Sílvia, Costa, Vera Marisa, Carmo, Helena, Carvalho, Márcia, Carvalho, Félix, Bastos, Maria de Lourdes, Lemos-Amado, Francisco, Remião, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10284/10050
Resumo: The sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity.
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spelling Evaluation of GSH adducts of adrenaline in biological samplesGSH adductsAdrenalineCatecholaminesOxidative stressHPLCThe sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity.John Wiley & SonsRepositório Institucional da Universidade Fernando PessoaSilva, RenataBoldt, SílviaCosta, Vera MarisaCarmo, HelenaCarvalho, MárciaCarvalho, FélixBastos, Maria de LourdesLemos-Amado, FranciscoRemião, Fernando2021-07-05T09:11:21Z2007-01-01T00:00:00Z2007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10284/10050eng0269-387910.1002/bmc.7961099-0801metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T02:09:20Zoai:bdigital.ufp.pt:10284/10050Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:46:49.465149Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of GSH adducts of adrenaline in biological samples
title Evaluation of GSH adducts of adrenaline in biological samples
spellingShingle Evaluation of GSH adducts of adrenaline in biological samples
Silva, Renata
GSH adducts
Adrenaline
Catecholamines
Oxidative stress
HPLC
title_short Evaluation of GSH adducts of adrenaline in biological samples
title_full Evaluation of GSH adducts of adrenaline in biological samples
title_fullStr Evaluation of GSH adducts of adrenaline in biological samples
title_full_unstemmed Evaluation of GSH adducts of adrenaline in biological samples
title_sort Evaluation of GSH adducts of adrenaline in biological samples
author Silva, Renata
author_facet Silva, Renata
Boldt, Sílvia
Costa, Vera Marisa
Carmo, Helena
Carvalho, Márcia
Carvalho, Félix
Bastos, Maria de Lourdes
Lemos-Amado, Francisco
Remião, Fernando
author_role author
author2 Boldt, Sílvia
Costa, Vera Marisa
Carmo, Helena
Carvalho, Márcia
Carvalho, Félix
Bastos, Maria de Lourdes
Lemos-Amado, Francisco
Remião, Fernando
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Institucional da Universidade Fernando Pessoa
dc.contributor.author.fl_str_mv Silva, Renata
Boldt, Sílvia
Costa, Vera Marisa
Carmo, Helena
Carvalho, Márcia
Carvalho, Félix
Bastos, Maria de Lourdes
Lemos-Amado, Francisco
Remião, Fernando
dc.subject.por.fl_str_mv GSH adducts
Adrenaline
Catecholamines
Oxidative stress
HPLC
topic GSH adducts
Adrenaline
Catecholamines
Oxidative stress
HPLC
description The sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01T00:00:00Z
2007-01-01T00:00:00Z
2021-07-05T09:11:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10284/10050
url http://hdl.handle.net/10284/10050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0269-3879
10.1002/bmc.796
1099-0801
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dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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