Advances in antitumor effects using liposomal citrinin in induced breast cancer model

• Autor(a) principal: Moura, Michely Laiany Vieira
• Data de Publicação: 2024
• Outros Autores: Menezes, Ag-Anne Pereira Melo de, Filho, José Williams Gomes de Oliveira, Nascimento, Maria Luiza Lima Barreto do, Reis, Antonielly Campinho dos, Ribeiro, Alessandra Braga, Silva, Felipe Cavalcanti Carneiro da, Nunes, Adriana Maria Viana, Rolim, Hercília Maria Lins, Cavalcante, Ana Amélia de Carvalho Melo, Sousa, João Marcelo de Castro e
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.14/43831
• topic: Fungal metabolites; Cytotoxicity; Nanotechnology; Breast cancer

The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). Mus musculus virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.