The central nervous system source modulates microglia function and morphology in vitro
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/85605 |
Resumo: | The regional heterogeneity of microglia was first described a century ago by Pio del Rio Hortega. Currently, new information on microglia heterogeneity throughout central nervous system (CNS) regions is being revealed by high-throughput techniques. It remains unclear whether these spatial specificities translate into different microglial behaviors in vitro. We cultured microglia isolated from the cortex and spinal cord and analyzed the effect of the CNS spatial source on behavior in vitro by applying the same experimental protocol and culture conditions. We analyzed the microglial cell numbers, function, and morphology and found a distinctive in vitro phenotype. We found that microglia were present in higher numbers in the spinal-cord-derived glial cultures, presenting different expressions of inflammatory genes and a lower phagocytosis rate under basal conditions or after activation with LPS and IFN-γ. Morphologically, the cortical microglial cells were more complex and presented longer ramifications, which were also observed in vivo in CX3CR1<sup>+/GFP</sup> transgenic reporter mice. Collectively, our data demonstrated that microglial behavior in vitro is defined according to specific spatial characteristics acquired by the tissue. Thus, our study highlights the importance of microglia as a source of CNS for in vitro studies. |
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The central nervous system source modulates microglia function and morphology in vitroMicrogliaCortexSpinal cordMorphologyPhagocytosisIn vitro studiesThe regional heterogeneity of microglia was first described a century ago by Pio del Rio Hortega. Currently, new information on microglia heterogeneity throughout central nervous system (CNS) regions is being revealed by high-throughput techniques. It remains unclear whether these spatial specificities translate into different microglial behaviors in vitro. We cultured microglia isolated from the cortex and spinal cord and analyzed the effect of the CNS spatial source on behavior in vitro by applying the same experimental protocol and culture conditions. We analyzed the microglial cell numbers, function, and morphology and found a distinctive in vitro phenotype. We found that microglia were present in higher numbers in the spinal-cord-derived glial cultures, presenting different expressions of inflammatory genes and a lower phagocytosis rate under basal conditions or after activation with LPS and IFN-γ. Morphologically, the cortical microglial cells were more complex and presented longer ramifications, which were also observed in vivo in CX3CR1<sup>+/GFP</sup> transgenic reporter mice. Collectively, our data demonstrated that microglial behavior in vitro is defined according to specific spatial characteristics acquired by the tissue. Thus, our study highlights the importance of microglia as a source of CNS for in vitro studies.This work was funded by the Santa Casa Neuroscience Awards—Prize Melo e Castro for Spinal Cord Injury Research (MC-18-2021), and it was partially funded by the Wings for Life Spinal Cord Research Foundation (WFL-ES-03/19). This work was also funded by national funds through the Foundation for Science and Technology (FCT)—projects UIDB/50026/2020, UIDP/50026/2020, and EXPL/MED-PAT/0931/2021, and by the project NORTE-01-0145-FEDER-000039, supported by the Norte Portugal Regional Operational Programme (NORTE 2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). We would like to acknowledge the support given by the Portuguese Foundation of Science and Technology to AGP (2020.07534.BD), AM (UMINHO/BIL-CNCG/2022/16), SM (CEECIND/01902/2017), and NAS (CEECIND/04794/2007).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoPinho, Andreia Filipa GomesMonteiro, Andreia Manuela FernandesFernandes, Sarade Sousa, NídiaSalgado, A. J.Silva, Nuno André MartinsMonteiro, Susana Isabel Gonçalves2023-04-222023-04-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85605engPinho, A.G.; Monteiro, A.; Fernandes, S.; de Sousa, N.; Salgado, A.J.; Silva, N.A.; Monteiro, S. The Central Nervous System Source Modulates Microglia Function and Morphology In Vitro. Int. J. Mol. Sci. 2023, 24, 7685. https://doi.org/10.3390/ijms240976851661-65961422-006710.3390/ijms2409768537175391https://www.mdpi.com/1422-0067/24/9/7685info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-22T01:16:05Zoai:repositorium.sdum.uminho.pt:1822/85605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:54:20.923926Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The central nervous system source modulates microglia function and morphology in vitro |
title |
The central nervous system source modulates microglia function and morphology in vitro |
spellingShingle |
The central nervous system source modulates microglia function and morphology in vitro Pinho, Andreia Filipa Gomes Microglia Cortex Spinal cord Morphology Phagocytosis In vitro studies |
title_short |
The central nervous system source modulates microglia function and morphology in vitro |
title_full |
The central nervous system source modulates microglia function and morphology in vitro |
title_fullStr |
The central nervous system source modulates microglia function and morphology in vitro |
title_full_unstemmed |
The central nervous system source modulates microglia function and morphology in vitro |
title_sort |
The central nervous system source modulates microglia function and morphology in vitro |
author |
Pinho, Andreia Filipa Gomes |
author_facet |
Pinho, Andreia Filipa Gomes Monteiro, Andreia Manuela Fernandes Fernandes, Sara de Sousa, Nídia Salgado, A. J. Silva, Nuno André Martins Monteiro, Susana Isabel Gonçalves |
author_role |
author |
author2 |
Monteiro, Andreia Manuela Fernandes Fernandes, Sara de Sousa, Nídia Salgado, A. J. Silva, Nuno André Martins Monteiro, Susana Isabel Gonçalves |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pinho, Andreia Filipa Gomes Monteiro, Andreia Manuela Fernandes Fernandes, Sara de Sousa, Nídia Salgado, A. J. Silva, Nuno André Martins Monteiro, Susana Isabel Gonçalves |
dc.subject.por.fl_str_mv |
Microglia Cortex Spinal cord Morphology Phagocytosis In vitro studies |
topic |
Microglia Cortex Spinal cord Morphology Phagocytosis In vitro studies |
description |
The regional heterogeneity of microglia was first described a century ago by Pio del Rio Hortega. Currently, new information on microglia heterogeneity throughout central nervous system (CNS) regions is being revealed by high-throughput techniques. It remains unclear whether these spatial specificities translate into different microglial behaviors in vitro. We cultured microglia isolated from the cortex and spinal cord and analyzed the effect of the CNS spatial source on behavior in vitro by applying the same experimental protocol and culture conditions. We analyzed the microglial cell numbers, function, and morphology and found a distinctive in vitro phenotype. We found that microglia were present in higher numbers in the spinal-cord-derived glial cultures, presenting different expressions of inflammatory genes and a lower phagocytosis rate under basal conditions or after activation with LPS and IFN-γ. Morphologically, the cortical microglial cells were more complex and presented longer ramifications, which were also observed in vivo in CX3CR1<sup>+/GFP</sup> transgenic reporter mice. Collectively, our data demonstrated that microglial behavior in vitro is defined according to specific spatial characteristics acquired by the tissue. Thus, our study highlights the importance of microglia as a source of CNS for in vitro studies. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-22 2023-04-22T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/85605 |
url |
https://hdl.handle.net/1822/85605 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinho, A.G.; Monteiro, A.; Fernandes, S.; de Sousa, N.; Salgado, A.J.; Silva, N.A.; Monteiro, S. The Central Nervous System Source Modulates Microglia Function and Morphology In Vitro. Int. J. Mol. Sci. 2023, 24, 7685. https://doi.org/10.3390/ijms24097685 1661-6596 1422-0067 10.3390/ijms24097685 37175391 https://www.mdpi.com/1422-0067/24/9/7685 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133142297083904 |