Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp

Detalhes bibliográficos
Autor(a) principal: Gongi, Wejdene
Data de Publicação: 2020
Outros Autores: Cordeiro, Nereida, Pinchetti, Juan Luis Gomez, Sadok, Saloua, Ben Ouada, Hatem
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/3281
Resumo: The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50 °C). A joint production of biomass reaching 0.50 gdw L−1 day−1 and of EPS production reaching 1.30 gdw L−1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.
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spelling Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella spMicroalgaeGraesiellaChlorophytaEPSsAntioxidant enzymesCharacterizationRadical scavengingOutdoor cultivationTwo-step strategy.Faculdade de Ciências Exatas e da EngenhariaThe present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50 °C). A joint production of biomass reaching 0.50 gdw L−1 day−1 and of EPS production reaching 1.30 gdw L−1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.SpringerDigitUMaGongi, WejdeneCordeiro, NereidaPinchetti, Juan Luis GomezSadok, SalouaBen Ouada, Hatem2021-04-13T13:37:21Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3281engGongi, W., Cordeiro, N., Pinchetti, J. L. G., Sadok, S., & Ouada, H. B. (2021). Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp. Journal of Applied Phycology, 33(1), 357-369.10.1007/s10811-020-02303-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-19T05:34:19Zoai:digituma.uma.pt:10400.13/3281Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:17.172833Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
title Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
spellingShingle Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
Gongi, Wejdene
Microalgae
Graesiella
Chlorophyta
EPSs
Antioxidant enzymes
Characterization
Radical scavenging
Outdoor cultivation
Two-step strategy
.
Faculdade de Ciências Exatas e da Engenharia
title_short Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
title_full Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
title_fullStr Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
title_full_unstemmed Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
title_sort Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
author Gongi, Wejdene
author_facet Gongi, Wejdene
Cordeiro, Nereida
Pinchetti, Juan Luis Gomez
Sadok, Saloua
Ben Ouada, Hatem
author_role author
author2 Cordeiro, Nereida
Pinchetti, Juan Luis Gomez
Sadok, Saloua
Ben Ouada, Hatem
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Gongi, Wejdene
Cordeiro, Nereida
Pinchetti, Juan Luis Gomez
Sadok, Saloua
Ben Ouada, Hatem
dc.subject.por.fl_str_mv Microalgae
Graesiella
Chlorophyta
EPSs
Antioxidant enzymes
Characterization
Radical scavenging
Outdoor cultivation
Two-step strategy
.
Faculdade de Ciências Exatas e da Engenharia
topic Microalgae
Graesiella
Chlorophyta
EPSs
Antioxidant enzymes
Characterization
Radical scavenging
Outdoor cultivation
Two-step strategy
.
Faculdade de Ciências Exatas e da Engenharia
description The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50 °C). A joint production of biomass reaching 0.50 gdw L−1 day−1 and of EPS production reaching 1.30 gdw L−1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-04-13T13:37:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/3281
url http://hdl.handle.net/10400.13/3281
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gongi, W., Cordeiro, N., Pinchetti, J. L. G., Sadok, S., & Ouada, H. B. (2021). Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp. Journal of Applied Phycology, 33(1), 357-369.
10.1007/s10811-020-02303-0
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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