The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/20.500.12207/6065 |
Resumo: | A study was carried out to determine the ALS (acetolactate synthase)-inhibitor herbicide resistance in the Mexican grass Ixophorus unisetus, a troublesome weed in corn crops in Mexico. First, the resistance was confirmed in field screening assays. Eight populations that survived nicosulfuron treatment at a field rate of 40 g ai ha−1 were labeled as putative-resistant. Dose–response trials demonstrated a high resistance in the eight populations (GR50 from 140.33 to 245.46 g ai ha−1 ). The synergism of malathion plus nicosulfuron demonstrated that the non-target-site resistance (NTSR) mechanism based on cytochrome P450 (Cyt-P450) was involved in five populations of I. unisetus. Molecular studies revealed that a single-nucleotide change occurs in the amino acid at position 376 (from GAT to GAG), which codifies from Asp-376 to Glu-376. This is the first time that Asp-376-Glu has been reported in this species. Assays in vitro and in vivo demonstrated I. unisetus cross-resistance to flucarbazone, penoxsulam, bispyribac-Na, and imazamox. No multiple resistance was found in two resistant populations exposed to different herbicides. Our results indicate that the lack of good control over Mexican grass in corn with ALS inhibitors is due to target-site mutation and NTSR mechanisms (Cyt-P450-mediated metabolism). A strategy should be established in Mexican fields to continue controlling this weed, including mechanical control practices and a good combination of the available pre- and post-emergence herbicides. |
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The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, MexicoCross-resistanceALS activityCorn weedsAsp376GluTarget-site mechanismNon-target-site mechanismA study was carried out to determine the ALS (acetolactate synthase)-inhibitor herbicide resistance in the Mexican grass Ixophorus unisetus, a troublesome weed in corn crops in Mexico. First, the resistance was confirmed in field screening assays. Eight populations that survived nicosulfuron treatment at a field rate of 40 g ai ha−1 were labeled as putative-resistant. Dose–response trials demonstrated a high resistance in the eight populations (GR50 from 140.33 to 245.46 g ai ha−1 ). The synergism of malathion plus nicosulfuron demonstrated that the non-target-site resistance (NTSR) mechanism based on cytochrome P450 (Cyt-P450) was involved in five populations of I. unisetus. Molecular studies revealed that a single-nucleotide change occurs in the amino acid at position 376 (from GAT to GAG), which codifies from Asp-376 to Glu-376. This is the first time that Asp-376-Glu has been reported in this species. Assays in vitro and in vivo demonstrated I. unisetus cross-resistance to flucarbazone, penoxsulam, bispyribac-Na, and imazamox. No multiple resistance was found in two resistant populations exposed to different herbicides. Our results indicate that the lack of good control over Mexican grass in corn with ALS inhibitors is due to target-site mutation and NTSR mechanisms (Cyt-P450-mediated metabolism). A strategy should be established in Mexican fields to continue controlling this weed, including mechanical control practices and a good combination of the available pre- and post-emergence herbicides.MDPI2023-11-30T11:25:03Z2023-06-22T00:00:00Z2023-06-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12207/6065eng2073-4395https://doi.org/10.3390/agronomy13071682Domínguez-Valenzuela, JoséVázquez-García, JoséCastro, PatriciaPalma-Bautista, CandelarioCruz-Hipolito, HugoRey, Maria-DoloresDe Prado, RafaelPortugal, Joãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-07T09:10:10Zoai:repositorio.ipbeja.pt:20.500.12207/6065Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:28.559614Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
title |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
spellingShingle |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico Domínguez-Valenzuela, José Cross-resistance ALS activity Corn weeds Asp376Glu Target-site mechanism Non-target-site mechanism |
title_short |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
title_full |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
title_fullStr |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
title_full_unstemmed |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
title_sort |
The Asp376Glu mutation and enhanced metabolism controlling the resistance of ALS-inhibiting herbicides in Ixophorus unisetus (J. Presl) Schltdl. from The Bajio, Mexico |
author |
Domínguez-Valenzuela, José |
author_facet |
Domínguez-Valenzuela, José Vázquez-García, José Castro, Patricia Palma-Bautista, Candelario Cruz-Hipolito, Hugo Rey, Maria-Dolores De Prado, Rafael Portugal, João |
author_role |
author |
author2 |
Vázquez-García, José Castro, Patricia Palma-Bautista, Candelario Cruz-Hipolito, Hugo Rey, Maria-Dolores De Prado, Rafael Portugal, João |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Domínguez-Valenzuela, José Vázquez-García, José Castro, Patricia Palma-Bautista, Candelario Cruz-Hipolito, Hugo Rey, Maria-Dolores De Prado, Rafael Portugal, João |
dc.subject.por.fl_str_mv |
Cross-resistance ALS activity Corn weeds Asp376Glu Target-site mechanism Non-target-site mechanism |
topic |
Cross-resistance ALS activity Corn weeds Asp376Glu Target-site mechanism Non-target-site mechanism |
description |
A study was carried out to determine the ALS (acetolactate synthase)-inhibitor herbicide resistance in the Mexican grass Ixophorus unisetus, a troublesome weed in corn crops in Mexico. First, the resistance was confirmed in field screening assays. Eight populations that survived nicosulfuron treatment at a field rate of 40 g ai ha−1 were labeled as putative-resistant. Dose–response trials demonstrated a high resistance in the eight populations (GR50 from 140.33 to 245.46 g ai ha−1 ). The synergism of malathion plus nicosulfuron demonstrated that the non-target-site resistance (NTSR) mechanism based on cytochrome P450 (Cyt-P450) was involved in five populations of I. unisetus. Molecular studies revealed that a single-nucleotide change occurs in the amino acid at position 376 (from GAT to GAG), which codifies from Asp-376 to Glu-376. This is the first time that Asp-376-Glu has been reported in this species. Assays in vitro and in vivo demonstrated I. unisetus cross-resistance to flucarbazone, penoxsulam, bispyribac-Na, and imazamox. No multiple resistance was found in two resistant populations exposed to different herbicides. Our results indicate that the lack of good control over Mexican grass in corn with ALS inhibitors is due to target-site mutation and NTSR mechanisms (Cyt-P450-mediated metabolism). A strategy should be established in Mexican fields to continue controlling this weed, including mechanical control practices and a good combination of the available pre- and post-emergence herbicides. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-30T11:25:03Z 2023-06-22T00:00:00Z 2023-06-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12207/6065 |
url |
https://hdl.handle.net/20.500.12207/6065 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4395 https://doi.org/10.3390/agronomy13071682 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136317862313984 |