Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi

Detalhes bibliográficos
Autor(a) principal: Archer, Margarida
Data de Publicação: 2000
Outros Autores: Rodrigues, Maria Luisa, Aurélio, M., Biemans, R., Cravador, A., Carrondo, Maria A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/1202
Resumo: Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.
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spelling Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomiBeta-cinnamominElicitinsPhytopathogenic fungiCinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.SapientiaArcher, MargaridaRodrigues, Maria LuisaAurélio, M.Biemans, R.Cravador, A.Carrondo, Maria A.2012-06-08T10:23:03Z20002012-06-07T17:47:28Z2000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/1202engArcher, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R; Cravador, Alfredo; Carrondo, Maria A.. Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi. Acta Crystallographica , 56, 3, 363-366, 2000.0907-4449AUT: ACR00659;info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:12:19Zoai:sapientia.ualg.pt:10400.1/1202Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:28.751977Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
title Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
spellingShingle Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
Archer, Margarida
Beta-cinnamomin
Elicitins
Phytopathogenic fungi
title_short Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
title_full Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
title_fullStr Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
title_full_unstemmed Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
title_sort Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
author Archer, Margarida
author_facet Archer, Margarida
Rodrigues, Maria Luisa
Aurélio, M.
Biemans, R.
Cravador, A.
Carrondo, Maria A.
author_role author
author2 Rodrigues, Maria Luisa
Aurélio, M.
Biemans, R.
Cravador, A.
Carrondo, Maria A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Archer, Margarida
Rodrigues, Maria Luisa
Aurélio, M.
Biemans, R.
Cravador, A.
Carrondo, Maria A.
dc.subject.por.fl_str_mv Beta-cinnamomin
Elicitins
Phytopathogenic fungi
topic Beta-cinnamomin
Elicitins
Phytopathogenic fungi
description Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
2012-06-08T10:23:03Z
2012-06-07T17:47:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/1202
url http://hdl.handle.net/10400.1/1202
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archer, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R; Cravador, Alfredo; Carrondo, Maria A.. Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi. Acta Crystallographica , 56, 3, 363-366, 2000.
0907-4449
AUT: ACR00659;
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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