Bioanalytical method validation of Venlafaxine and Desvenlafaxine in mouse plasma, brain and liver using a MEPS/HPLC assay: a path to study these drugs’ intranasal administration

• Autor(a) principal: Meirinho, Sara Alexandra
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.6/6438
• topic: Desvenlafaxine; Hplc; Intranasal Administration; Meps; Pharmacy; Venlafaxine; Domínio/Área Científica::Ciências Médicas::Ciências da Saúde::Ciências Farmacêuticas

The present thesis was elaborated aiming to obtain the master’s degree in pharmaceutical sciences. In here, it is described all the scientific investigation work done to reach the proposed objective, as well as all tasks and learnings that I have taken along my community and hospital pharmacy internships. Chapter 1 is related with my scientific investigation work entitled “Bioanalytical method validation of Venlafaxine and Desvenlafaxine in mouse plasma, brain and liver using a MEPS/HPLC assay: a path to study these drugs’ intranasal administration”. Since major depressive disorder is one of the most prevalent psychiatric diseases, its therapeutic successful rate must be increased. Nowadays, the majority of all antidepressants used are given orally, being this route a source of therapeutic problems, mainly in what concerns with the time and dosage required to accomplish the desired effects. So, in order to improve it, intranasal administration route has been investigated, once parts of the nasal cavity directly contacts with brain tissue. Over time, and after some research, it was also understood that Venlafaxine is one of the most successful antidepressants until now, having the advantage of being metabolized to an active metabolite called Desvenlafaxine. So, in order to perform some future studies using intranasally administration of both these drugs and understand what advantages it brings, a reliable bioanalytical quantification method for venlafaxine and desvenlafaxine in mouse plasma, brain and liver matrices must be validated, in this case, using a MEPS/HPLC method. Chapter 2 is related with community pharmacy internship, describing all the performed activities during the 3 month that I have stayed in Pharmacy Alameda. The focus in this chapter is to understand how community pharmacies in general work, taking into account not only drugs dispense and pharmaceutical services but also the best way to manage them. Chapter 3 is about my 2 month of internship in hospital pharmacy services, describing all the duties and tasks performed in each pharmacy section. In here it is also explicit pharmacists’ importance in patients’ pharmaceutical therapies management as well as in controlling all the economic costs related with it.