Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/33655 |
Resumo: | Passive immune transfer (PIT) is detrimental to the calf’s welfare. PIT is currently assessed by immunoglobulins or total proteins concentration (TP) in blood. Blood collection has some constraints, ergo, a simple and non-invasive alternative method is of great interest. This study aimed to evaluate the potential use of saliva as a non-invasive fluid to assess PIT. A total of eighty saliva and blood samples were taken from twenty calves at 4 time points: at birth (approximately 30 min before colostrum consumption), at 24h, 48h and at day 7, for total protein and IgG levels concentration assessment. A hand-held refractometer was used for serum samples and the Bradford method for total protein determination in both saliva and serum samples. Dot-blot analysis was performed in a sub-sample to check for IgG in saliva and serum (n=5 calves). With both methods, TP in serum at 24h, 48h and at day 7 was significative higher than TP at birth (P<0.001). A tendency for elevated TP at 24h (P<0.1) was found in saliva samples. A significant Pearson’s positive correlation was found between TP in saliva and in serum by refractometer at 48h (r=0.45; P=0.0474). Dot-blot analysis showed an increase in IgG from birth to following phases in blood and saliva. These results suggest that saliva can be used for PIT surveillance, highlighting the importance of further studies. |
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Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calvessalivacalvespassive immune transfernon-invasivePassive immune transfer (PIT) is detrimental to the calf’s welfare. PIT is currently assessed by immunoglobulins or total proteins concentration (TP) in blood. Blood collection has some constraints, ergo, a simple and non-invasive alternative method is of great interest. This study aimed to evaluate the potential use of saliva as a non-invasive fluid to assess PIT. A total of eighty saliva and blood samples were taken from twenty calves at 4 time points: at birth (approximately 30 min before colostrum consumption), at 24h, 48h and at day 7, for total protein and IgG levels concentration assessment. A hand-held refractometer was used for serum samples and the Bradford method for total protein determination in both saliva and serum samples. Dot-blot analysis was performed in a sub-sample to check for IgG in saliva and serum (n=5 calves). With both methods, TP in serum at 24h, 48h and at day 7 was significative higher than TP at birth (P<0.001). A tendency for elevated TP at 24h (P<0.1) was found in saliva samples. A significant Pearson’s positive correlation was found between TP in saliva and in serum by refractometer at 48h (r=0.45; P=0.0474). Dot-blot analysis showed an increase in IgG from birth to following phases in blood and saliva. These results suggest that saliva can be used for PIT surveillance, highlighting the importance of further studies.EAAP - Federação Europeia de Ciência Animal2023-01-25T16:10:56Z2023-01-252022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/33655http://hdl.handle.net/10174/33655porSilva, F.G., Lamy, E., Pedro, S., Azevedo, I., Caetano, P., Ramalho, J., Martins, L., Cerqueira, J.O.L., Silva, S.R., Conceição, C., 2022. Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves. 73º Congresso Anual da Federação Europeia de Ciência Animal, de 05 a 09 de setembro de 2022. Livro de resumos N. 28, pág. 240. ISBN: 978-90-8686-385-3.simnaonaofsilva@uevora.ptecsl@uevora.ptndndndjoana.ramalho94@gmail.comlmlm@uevora.ptcerqueira@esa.ipvc.ptssilva@utad.ptcristinaconceicao@uevora.ptSilva, FlávioLamy, ElsaPedro, SofiaAzevedo, InêsCaetano, PedroRamalho, JoanaMartins, LuísCerqueira, JoaquimSilva, SeverianoConceição, Cristinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:35:29Zoai:dspace.uevora.pt:10174/33655Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:21.860896Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
title |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
spellingShingle |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves Silva, Flávio saliva calves passive immune transfer non-invasive |
title_short |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
title_full |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
title_fullStr |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
title_full_unstemmed |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
title_sort |
Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves |
author |
Silva, Flávio |
author_facet |
Silva, Flávio Lamy, Elsa Pedro, Sofia Azevedo, Inês Caetano, Pedro Ramalho, Joana Martins, Luís Cerqueira, Joaquim Silva, Severiano Conceição, Cristina |
author_role |
author |
author2 |
Lamy, Elsa Pedro, Sofia Azevedo, Inês Caetano, Pedro Ramalho, Joana Martins, Luís Cerqueira, Joaquim Silva, Severiano Conceição, Cristina |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Flávio Lamy, Elsa Pedro, Sofia Azevedo, Inês Caetano, Pedro Ramalho, Joana Martins, Luís Cerqueira, Joaquim Silva, Severiano Conceição, Cristina |
dc.subject.por.fl_str_mv |
saliva calves passive immune transfer non-invasive |
topic |
saliva calves passive immune transfer non-invasive |
description |
Passive immune transfer (PIT) is detrimental to the calf’s welfare. PIT is currently assessed by immunoglobulins or total proteins concentration (TP) in blood. Blood collection has some constraints, ergo, a simple and non-invasive alternative method is of great interest. This study aimed to evaluate the potential use of saliva as a non-invasive fluid to assess PIT. A total of eighty saliva and blood samples were taken from twenty calves at 4 time points: at birth (approximately 30 min before colostrum consumption), at 24h, 48h and at day 7, for total protein and IgG levels concentration assessment. A hand-held refractometer was used for serum samples and the Bradford method for total protein determination in both saliva and serum samples. Dot-blot analysis was performed in a sub-sample to check for IgG in saliva and serum (n=5 calves). With both methods, TP in serum at 24h, 48h and at day 7 was significative higher than TP at birth (P<0.001). A tendency for elevated TP at 24h (P<0.1) was found in saliva samples. A significant Pearson’s positive correlation was found between TP in saliva and in serum by refractometer at 48h (r=0.45; P=0.0474). Dot-blot analysis showed an increase in IgG from birth to following phases in blood and saliva. These results suggest that saliva can be used for PIT surveillance, highlighting the importance of further studies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01T00:00:00Z 2023-01-25T16:10:56Z 2023-01-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/33655 http://hdl.handle.net/10174/33655 |
url |
http://hdl.handle.net/10174/33655 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Silva, F.G., Lamy, E., Pedro, S., Azevedo, I., Caetano, P., Ramalho, J., Martins, L., Cerqueira, J.O.L., Silva, S.R., Conceição, C., 2022. Saliva as a potential non-invasive fluid for passive immune transfer surveillance in calves. 73º Congresso Anual da Federação Europeia de Ciência Animal, de 05 a 09 de setembro de 2022. Livro de resumos N. 28, pág. 240. ISBN: 978-90-8686-385-3. sim nao nao fsilva@uevora.pt ecsl@uevora.pt nd nd nd joana.ramalho94@gmail.com lmlm@uevora.pt cerqueira@esa.ipvc.pt ssilva@utad.pt cristinaconceicao@uevora.pt |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
EAAP - Federação Europeia de Ciência Animal |
publisher.none.fl_str_mv |
EAAP - Federação Europeia de Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136705530298368 |