Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method

Detalhes bibliográficos
Autor(a) principal: Martins, Tiago M.
Data de Publicação: 2008
Outros Autores: Pedro, Olívia C., Caldeira, Rubina A., do Rosário, Virgílio E., Neves, Luís, Domingos, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145213
Resumo: Funding Information: Tiago Martins would like to acknowledge Fundação para a Ciência e a Tecnologia (FCT), Portugal, for his PhD grant. We would like to recognize Dr. Varda Shkap from Kimron Veterinary Institute, Israel, for the kind donation of high quality genomic DNA from B. bovis and B. bigemina.
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spelling Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR methodAspartic proteaseB. bovisBabesia bigeminaHot-start PCRParasitologyveterinary(all)Funding Information: Tiago Martins would like to acknowledge Fundação para a Ciência e a Tecnologia (FCT), Portugal, for his PhD grant. We would like to recognize Dr. Varda Shkap from Kimron Veterinary Institute, Israel, for the kind donation of high quality genomic DNA from B. bovis and B. bigemina.Babesiosis is a tick borne disease (TBD) caused by parasites of the genus Babesia, with considerable worldwide economic, medical, and veterinary impact. Bovine babesiosis and other TBDs were considered responsible for 50% of the deaths of cattle that occurred in Mozambique in the first year after importation from neighbouring countries. Here, we present the detection of Babesia bigemina and Babesia bovis in cattle from Mozambique using two distinct PCR methods. For this study, blood samples were collected in one farm located near Maputo city. The DNA samples were analyzed using a previously described nested PCR and a novel hot-start PCR method. Primers were selected for the hot-start PCR based on the putative gene of an undescribed aspartic protease named babesipsin, present in both B. bovis and B. bigemina. The combination of hot-start polymerase and long primers (29-31 bp) were in this study determinant for the successful amplification and detection in only one PCR. With a seminested approach the sensitivity was further increased. The babesipsin seminested hot-start PCR was in this study more sensitive than the nested PCR. A total of 117 field samples were tested by seminested hot-start PCR, and 104 were positive for B. bigemina (90%), 97 were positive for B. bovis (82%), 86 were mixed infections (52%) and only 2 were negative for both Babesia species (1.7%). The results confirm that this area of Mozambique is endemic for babesiosis, and that this TBD should be regarded as a threat for imported cattle.Centro de Malária e outras Doenças Tropicais (CMDT)RUNMartins, Tiago M.Pedro, Olívia C.Caldeira, Rubina A.do Rosário, Virgílio E.Neves, LuísDomingos, Ana2022-11-03T22:13:13Z2008-05-312008-05-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/145213eng0304-4017PURE: 46706524https://doi.org/10.1016/j.vetpar.2008.01.037info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:22Zoai:run.unl.pt:10362/145213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:57.798228Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
title Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
spellingShingle Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
Martins, Tiago M.
Aspartic protease
B. bovis
Babesia bigemina
Hot-start PCR
Parasitology
veterinary(all)
title_short Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
title_full Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
title_fullStr Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
title_full_unstemmed Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
title_sort Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method
author Martins, Tiago M.
author_facet Martins, Tiago M.
Pedro, Olívia C.
Caldeira, Rubina A.
do Rosário, Virgílio E.
Neves, Luís
Domingos, Ana
author_role author
author2 Pedro, Olívia C.
Caldeira, Rubina A.
do Rosário, Virgílio E.
Neves, Luís
Domingos, Ana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Malária e outras Doenças Tropicais (CMDT)
RUN
dc.contributor.author.fl_str_mv Martins, Tiago M.
Pedro, Olívia C.
Caldeira, Rubina A.
do Rosário, Virgílio E.
Neves, Luís
Domingos, Ana
dc.subject.por.fl_str_mv Aspartic protease
B. bovis
Babesia bigemina
Hot-start PCR
Parasitology
veterinary(all)
topic Aspartic protease
B. bovis
Babesia bigemina
Hot-start PCR
Parasitology
veterinary(all)
description Funding Information: Tiago Martins would like to acknowledge Fundação para a Ciência e a Tecnologia (FCT), Portugal, for his PhD grant. We would like to recognize Dr. Varda Shkap from Kimron Veterinary Institute, Israel, for the kind donation of high quality genomic DNA from B. bovis and B. bigemina.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-31
2008-05-31T00:00:00Z
2022-11-03T22:13:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145213
url http://hdl.handle.net/10362/145213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0304-4017
PURE: 46706524
https://doi.org/10.1016/j.vetpar.2008.01.037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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