Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53

Detalhes bibliográficos
Autor(a) principal: Rodrigues, L. R.
Data de Publicação: 2006
Outros Autores: Teixeira, J. A., Mei, Henny van der, Oliveira, Rosário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/5106
Resumo: Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved for the isolated fraction was 14 g/l, allowing for a surface tension value of 36 mJ/m². Moreover, the isolated fraction, stable to pH changes between 5 and 9, was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses, even at low concentrations. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered.
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spelling Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53BiosurfactantLactococcus lactis 53IsolationPhysicochemical characterizationScience & TechnologyIsolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved for the isolated fraction was 14 g/l, allowing for a surface tension value of 36 mJ/m². Moreover, the isolated fraction, stable to pH changes between 5 and 9, was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses, even at low concentrations. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered.Fundação para a Ciência e a Tecnologia (FCT) ; CACTI (Spain); Joop de Vries (University of Groningen, The Netherlands).ElsevierUniversidade do MinhoRodrigues, L. R.Teixeira, J. A.Mei, Henny van derOliveira, Rosário20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5106eng"Colloids and Surfaces B : Biointerfaces". ISSN 0927-7765. 49:1 (2006) 78-85.0927-776510.1016/j.colsurfb.2006.03.00316616461info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:49Zoai:repositorium.sdum.uminho.pt:1822/5106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:52.933783Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
title Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
spellingShingle Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
Rodrigues, L. R.
Biosurfactant
Lactococcus lactis 53
Isolation
Physicochemical characterization
Science & Technology
title_short Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
title_full Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
title_fullStr Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
title_full_unstemmed Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
title_sort Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53
author Rodrigues, L. R.
author_facet Rodrigues, L. R.
Teixeira, J. A.
Mei, Henny van der
Oliveira, Rosário
author_role author
author2 Teixeira, J. A.
Mei, Henny van der
Oliveira, Rosário
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, L. R.
Teixeira, J. A.
Mei, Henny van der
Oliveira, Rosário
dc.subject.por.fl_str_mv Biosurfactant
Lactococcus lactis 53
Isolation
Physicochemical characterization
Science & Technology
topic Biosurfactant
Lactococcus lactis 53
Isolation
Physicochemical characterization
Science & Technology
description Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved for the isolated fraction was 14 g/l, allowing for a surface tension value of 36 mJ/m². Moreover, the isolated fraction, stable to pH changes between 5 and 9, was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses, even at low concentrations. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/5106
url http://hdl.handle.net/1822/5106
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Colloids and Surfaces B : Biointerfaces". ISSN 0927-7765. 49:1 (2006) 78-85.
0927-7765
10.1016/j.colsurfb.2006.03.003
16616461
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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