Potential application of endophytes in the management of olive fruit fly and anthracnose
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24257 |
Resumo: | Olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), and olive anthracnose, mainly caused by Colletotrichum acutatum, are considered key biotic constrains of olive crop worldwide. Strategies employed for controlling this pest and disease are mostly based on the use of insecticides and copper-based pesticides, respectively, which is not compatible with sustainable production systems. This work aims to study the effect of two endophytes, previously isolated from olive tree, namely Aureobasidium pullulans and Sarocladium summerbellii on the behavior (i.e. attractiveness or repellence) and preference for oviposition of B. oleae, as well as on anthracnose infection, by using in vitro and in vivo (detached fruits) assays. Aureobasidium. pullulans-mediated changes in olive fruit volatile organic compounds (VOCs) and their consequences for anthracnose disease development were also evaluated. The tests performed by multi-choice olfactometer showed that both endophytes did not exert a repellent or attractive effect against mature gravid B. oleae female’s. Similarly, females did not showed preference to oviposit in olives inoculated with endophytes versus non-inoculated. Among the two endophytes tested, only A. pullulans inhibited significantly the growth (up to 1.3-fold), sporulation (up to 5.9-fold) and spore germination (up to 3.5-fold) of C. acutatum in relation to the control (C. acutatum). Similarly, in inoculated olive assays, only A. pullulans was able to reduced significantly progress curve for incidence (up to 10-fold) and severity (up to 35-fold) of anthracnose, as well as both sporulation (up to 90%) and germination (up to 70%) of C. acutatum, in comparison with olives inoculated solely with C. acutatum. Gas chromatography–mass spectrometry analysis of olives inoculated with A. pullulans+C. acutatum and controls (olives inoculated with C. acutatum, A. pullulans or tween) led to identification of 29 VOCs, belonging to 10 different chemical classes, being alcohols the most diversified and abundant ones. The volatile profile of A. pullulans + C. acutatum revealed quali- and quantitatively differences from the controls, in particular due to the production of (z)-3-hexen-1-ol and acetic acid, hexyl ester. These findings provide new viable possibilities of controlling olive anthracnose using the endophyte A. pullulans and/or their VOCs. These volatile inhibitory metabolites should be further tested against anthracnose. |
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Potential application of endophytes in the management of olive fruit fly and anthracnoseBiocontrolAureobasidium pullulansSarocladium summerbelliiBactrocera oleaeColletotrichum acutatumVolatile compoundsDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasOlive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), and olive anthracnose, mainly caused by Colletotrichum acutatum, are considered key biotic constrains of olive crop worldwide. Strategies employed for controlling this pest and disease are mostly based on the use of insecticides and copper-based pesticides, respectively, which is not compatible with sustainable production systems. This work aims to study the effect of two endophytes, previously isolated from olive tree, namely Aureobasidium pullulans and Sarocladium summerbellii on the behavior (i.e. attractiveness or repellence) and preference for oviposition of B. oleae, as well as on anthracnose infection, by using in vitro and in vivo (detached fruits) assays. Aureobasidium. pullulans-mediated changes in olive fruit volatile organic compounds (VOCs) and their consequences for anthracnose disease development were also evaluated. The tests performed by multi-choice olfactometer showed that both endophytes did not exert a repellent or attractive effect against mature gravid B. oleae female’s. Similarly, females did not showed preference to oviposit in olives inoculated with endophytes versus non-inoculated. Among the two endophytes tested, only A. pullulans inhibited significantly the growth (up to 1.3-fold), sporulation (up to 5.9-fold) and spore germination (up to 3.5-fold) of C. acutatum in relation to the control (C. acutatum). Similarly, in inoculated olive assays, only A. pullulans was able to reduced significantly progress curve for incidence (up to 10-fold) and severity (up to 35-fold) of anthracnose, as well as both sporulation (up to 90%) and germination (up to 70%) of C. acutatum, in comparison with olives inoculated solely with C. acutatum. Gas chromatography–mass spectrometry analysis of olives inoculated with A. pullulans+C. acutatum and controls (olives inoculated with C. acutatum, A. pullulans or tween) led to identification of 29 VOCs, belonging to 10 different chemical classes, being alcohols the most diversified and abundant ones. The volatile profile of A. pullulans + C. acutatum revealed quali- and quantitatively differences from the controls, in particular due to the production of (z)-3-hexen-1-ol and acetic acid, hexyl ester. These findings provide new viable possibilities of controlling olive anthracnose using the endophyte A. pullulans and/or their VOCs. These volatile inhibitory metabolites should be further tested against anthracnose.A mosca da azeitona, Bactrocera oleae (Rossi) (Diptera: Tephritidae), e a gafa, causada principalmente por Colletotrichum acutatum, são consideradas inimigos chave da oliveira em todo o mundo. As estratégias de luta contra esta praga e doença baseiam-se principalmente no uso de inseticidas e pesticidas à base de cobre, respetivamente, o que não é compatível com sistemas de produção sustentáveis. O presente trabalho tem como objetivo estudar o efeito de dois endófitos, previamente isolados de oliveira, nomeadamente Aureobasidium pullulans e Sarocladium summerbellii, sobre o comportamento (atratividade ou repelência) e preferência de frutos para oviposição de B. oleae, bem como sobre o desenvolvimento de gafa, utilizando ensaios in vitro e in vivo (frutos destacados). A emissão de voláteis de azeitonas inoculadas com A. pullulans e o seu efeito no desenvolvimento da gafa, foi igualmente estudado. Os ensaios de escolha múltipla realizados no olfatómetro mostraram que nenhum dos endófitos exerceu um efeito repelente ou atraente sobre fêmeas grávidas de B. oleae. De igual modo, não foi detetada nenhuma preferência pelas fêmeas em colocar ovos em frutos não inoculados versus inoculados com endófitos. Dos dois endófitos testados, apenas A. pullulans inibiu significativamente o crescimento (até 1,3 vezes), esporulação (até 5,9 vezes) e germinação (até 3,5 vezes) de C. acutatum em relação ao controlo (C. acutatum). Nos ensaios de inoculação de azeitonas, foi também verificado que apenas A. pullulans reduziu significativamente a curva de progresso para incidência (até 10 vezes) e severidade (até 35 vezes) da gafa, bem como a esporulação (até 90%) e germinação (até 70%) de C. acutatum, face a azeitonas inoculadas apenas com C. acutatum. Análise por cromatografia gasosa de azeitonas inoculadas com A. pullulans + C. acutatum e controlos (azeitonas inoculadas com C. acutatum, A. pullulans ou tween) permitiram identificar 29 compostos voláteis, pertencentes a 10 classes químicas, tendo sido os álcoois os mais diversificados e abundantes. O perfil volátil de A. pullulans + C. acutatum revelou diferenças qualitativas e quantitativas em relação aos controlos, em particular devido à produção de (z) -3-hexen-1-ol e ácido acético, hexil éster. Apesar de preliminares, os resultados obtidos oferecem novas possibilidades para a luta da gafa pela exploração do endófito A. pullulans e / ou dos seus voláteis. A ação e potencial destes voláteis na luta contra a gafa deverá ser estudada no futuro.This work is supported by FEDER funds through the COMPETE (Operational Program for Competitiveness Factors) and by National funds through the FCT (Foundation for Science and Technology) in the scope of the project POCI-01-0145-FEDER-031133 “MicOlives - Exploiting plant induced resistance by beneficial fungi as a new sustainable approach to olive crop protection”Baptista, PaulaPereira, J.A.Biblioteca Digital do IPBSdiri, Yosra2023-01-10T01:30:24Z202120202021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10198/24257TID:202794466enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:13Zoai:bibliotecadigital.ipb.pt:10198/24257Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:06.470810Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
title |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
spellingShingle |
Potential application of endophytes in the management of olive fruit fly and anthracnose Sdiri, Yosra Biocontrol Aureobasidium pullulans Sarocladium summerbellii Bactrocera oleae Colletotrichum acutatum Volatile compounds Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
title_full |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
title_fullStr |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
title_full_unstemmed |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
title_sort |
Potential application of endophytes in the management of olive fruit fly and anthracnose |
author |
Sdiri, Yosra |
author_facet |
Sdiri, Yosra |
author_role |
author |
dc.contributor.none.fl_str_mv |
Baptista, Paula Pereira, J.A. Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Sdiri, Yosra |
dc.subject.por.fl_str_mv |
Biocontrol Aureobasidium pullulans Sarocladium summerbellii Bactrocera oleae Colletotrichum acutatum Volatile compounds Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Biocontrol Aureobasidium pullulans Sarocladium summerbellii Bactrocera oleae Colletotrichum acutatum Volatile compounds Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
Olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), and olive anthracnose, mainly caused by Colletotrichum acutatum, are considered key biotic constrains of olive crop worldwide. Strategies employed for controlling this pest and disease are mostly based on the use of insecticides and copper-based pesticides, respectively, which is not compatible with sustainable production systems. This work aims to study the effect of two endophytes, previously isolated from olive tree, namely Aureobasidium pullulans and Sarocladium summerbellii on the behavior (i.e. attractiveness or repellence) and preference for oviposition of B. oleae, as well as on anthracnose infection, by using in vitro and in vivo (detached fruits) assays. Aureobasidium. pullulans-mediated changes in olive fruit volatile organic compounds (VOCs) and their consequences for anthracnose disease development were also evaluated. The tests performed by multi-choice olfactometer showed that both endophytes did not exert a repellent or attractive effect against mature gravid B. oleae female’s. Similarly, females did not showed preference to oviposit in olives inoculated with endophytes versus non-inoculated. Among the two endophytes tested, only A. pullulans inhibited significantly the growth (up to 1.3-fold), sporulation (up to 5.9-fold) and spore germination (up to 3.5-fold) of C. acutatum in relation to the control (C. acutatum). Similarly, in inoculated olive assays, only A. pullulans was able to reduced significantly progress curve for incidence (up to 10-fold) and severity (up to 35-fold) of anthracnose, as well as both sporulation (up to 90%) and germination (up to 70%) of C. acutatum, in comparison with olives inoculated solely with C. acutatum. Gas chromatography–mass spectrometry analysis of olives inoculated with A. pullulans+C. acutatum and controls (olives inoculated with C. acutatum, A. pullulans or tween) led to identification of 29 VOCs, belonging to 10 different chemical classes, being alcohols the most diversified and abundant ones. The volatile profile of A. pullulans + C. acutatum revealed quali- and quantitatively differences from the controls, in particular due to the production of (z)-3-hexen-1-ol and acetic acid, hexyl ester. These findings provide new viable possibilities of controlling olive anthracnose using the endophyte A. pullulans and/or their VOCs. These volatile inhibitory metabolites should be further tested against anthracnose. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2021 2021-01-01T00:00:00Z 2023-01-10T01:30:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
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http://hdl.handle.net/10198/24257 TID:202794466 |
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http://hdl.handle.net/10198/24257 |
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TID:202794466 |
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eng |
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openAccess |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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