A DNA barcode reference library of Portuguese mosquitoes

Detalhes bibliográficos
Autor(a) principal: Madeira, Sara
Data de Publicação: 2021
Outros Autores: Duarte, Ana, Boinas, Fernando, Osório, Hugo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22480
Resumo: Research Areas: Public, Environmental & Occupational Health ; Infectious Diseases ; Veterinary Sciences
id RCAP_af7b7d60383bdcefb0fa2910d5927e7f
oai_identifier_str oai:www.repository.utl.pt:10400.5/22480
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A DNA barcode reference library of Portuguese mosquitoesCulicidaeCytochrome oxidase I (COI)DNA barcodingMosquito surveillancePortugalSpecies identificationResearch Areas: Public, Environmental & Occupational Health ; Infectious Diseases ; Veterinary SciencesMosquitoes are important biological vectors of pathogens and species identification in all life stages is the first step for effective monitoring and control of mosquitoborne diseases. Molecular methods for species identification have been developed over the last years to overcome the limitations of the taxonomic identification based on morphology. DNA barcoding, using a fragment of the mitochondrial cytochrome oxidase I (COI) gene, can be used for species identification but a reliable and comprehensive reference database of verified sequences is required. In this study, we aimed to generate a DNA barcode reference library for the identification of mosquito species from Portuguese mosquito fauna, including most relevant vector species. Mosquitoes captured under the National Vector Surveillance Program (REVIVE) were processed for DNA extraction, COI gene fragment amplification and sequencing. Nighty-eight barcode sequences were obtained, representing 26 species and 6 genera. Sequences were submitted to GenBank and BOLD and were used for validation of phenetic classification. Barcode Index Number (BIN) assignment and Automatic Barcode Gap Discovery (ABGD) were used and clustered COI sequences into twenty-five molecular operational taxonomic units (MOTUs). This is the first comprehensive study that combines morphological and molecular identification of most mosquito species present in Portugal aiming to offer a reliable framework for mosquito species identification.WileyRepositório da Universidade de LisboaMadeira, SaraDuarte, AnaBoinas, FernandoOsório, Hugo2021-11-03T16:17:31Z2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22480engMadeira S, Duarte A, Boinas F, Osorio HC. 2021. A DNA barcode reference library of Portuguese mosquitoes. Zoonoses Public Health 68:926–936. DOI:10.1111/zph.128851863-195910.1111/zph.128851863-2378info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:00Zoai:www.repository.utl.pt:10400.5/22480Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:53.827205Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A DNA barcode reference library of Portuguese mosquitoes
title A DNA barcode reference library of Portuguese mosquitoes
spellingShingle A DNA barcode reference library of Portuguese mosquitoes
Madeira, Sara
Culicidae
Cytochrome oxidase I (COI)
DNA barcoding
Mosquito surveillance
Portugal
Species identification
title_short A DNA barcode reference library of Portuguese mosquitoes
title_full A DNA barcode reference library of Portuguese mosquitoes
title_fullStr A DNA barcode reference library of Portuguese mosquitoes
title_full_unstemmed A DNA barcode reference library of Portuguese mosquitoes
title_sort A DNA barcode reference library of Portuguese mosquitoes
author Madeira, Sara
author_facet Madeira, Sara
Duarte, Ana
Boinas, Fernando
Osório, Hugo
author_role author
author2 Duarte, Ana
Boinas, Fernando
Osório, Hugo
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Madeira, Sara
Duarte, Ana
Boinas, Fernando
Osório, Hugo
dc.subject.por.fl_str_mv Culicidae
Cytochrome oxidase I (COI)
DNA barcoding
Mosquito surveillance
Portugal
Species identification
topic Culicidae
Cytochrome oxidase I (COI)
DNA barcoding
Mosquito surveillance
Portugal
Species identification
description Research Areas: Public, Environmental & Occupational Health ; Infectious Diseases ; Veterinary Sciences
publishDate 2021
dc.date.none.fl_str_mv 2021-11-03T16:17:31Z
2021-08
2021-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22480
url http://hdl.handle.net/10400.5/22480
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Madeira S, Duarte A, Boinas F, Osorio HC. 2021. A DNA barcode reference library of Portuguese mosquitoes. Zoonoses Public Health 68:926–936. DOI:10.1111/zph.12885
1863-1959
10.1111/zph.12885
1863-2378
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1817552352825049088

Registros relacionados