The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/7734 |
Resumo: | Application of antimicrobial chemicals is a general procedure in the cleaning and disinfection of food-contacting surfaces. Adhesion to glass surfaces and chemically induced detachment of Pseudomonas fluorescens ATCC 13525T were studied in situ, under flow conditions, in a well-controlled parallel plate flow chamber (PPFC). Ortho-phthalaldehyde (OPA) and cetyltrimethyl ammonium bromide (CTAB) were applied separately, at several concentrations, to attached bacteria and their subsequent detachment was monitored. Following treatments the remaining adhered bacteria were characterized in terms of viability and cell size. Simultaneously, the planktonic cell surface was characterized in order to correlate PPFC results with thermodynamic approaches for adhesion evaluation, and surface free energy of chemically treated cells with adhesion strength. About 2.8 × 106 cells/cm2 adhered to the glass surface after 30 min of bacterial flow, although thermodynamic analyses evidenced unfavourable adhesion. The independent application of OPA and CTAB promoted bacterial detachment to a small extent (16% of total cells). The remaining adhering bacteria were totally non-viable for OPA ≥ 0.75 mM and CTAB ≥ 0.25 mM, showing a lack of correlation between bacterial viability and detachment. The cellular size decreased as attachment proceeded and with chemical treatment. Both chemicals altered the cell surface properties, increasing the cell-glass adhesion strength, and promoting the emergence of polar characteristics. The overall results emphasize that OPA and CTAB were markedly ineffective in removing glass-attached P. fluorescens, demonstrating that bacteria can be non-viable but remain strongly attached to the adhesion surface. |
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The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfacesBiofilmsCetyltrimethyl ammonium bromideDetachmentDisinfectionOrtho-phthalaldehydePseudomonas fluorescensortho-phthaialdehydeScience & TechnologyApplication of antimicrobial chemicals is a general procedure in the cleaning and disinfection of food-contacting surfaces. Adhesion to glass surfaces and chemically induced detachment of Pseudomonas fluorescens ATCC 13525T were studied in situ, under flow conditions, in a well-controlled parallel plate flow chamber (PPFC). Ortho-phthalaldehyde (OPA) and cetyltrimethyl ammonium bromide (CTAB) were applied separately, at several concentrations, to attached bacteria and their subsequent detachment was monitored. Following treatments the remaining adhered bacteria were characterized in terms of viability and cell size. Simultaneously, the planktonic cell surface was characterized in order to correlate PPFC results with thermodynamic approaches for adhesion evaluation, and surface free energy of chemically treated cells with adhesion strength. About 2.8 × 106 cells/cm2 adhered to the glass surface after 30 min of bacterial flow, although thermodynamic analyses evidenced unfavourable adhesion. The independent application of OPA and CTAB promoted bacterial detachment to a small extent (16% of total cells). The remaining adhering bacteria were totally non-viable for OPA ≥ 0.75 mM and CTAB ≥ 0.25 mM, showing a lack of correlation between bacterial viability and detachment. The cellular size decreased as attachment proceeded and with chemical treatment. Both chemicals altered the cell surface properties, increasing the cell-glass adhesion strength, and promoting the emergence of polar characteristics. The overall results emphasize that OPA and CTAB were markedly ineffective in removing glass-attached P. fluorescens, demonstrating that bacteria can be non-viable but remain strongly attached to the adhesion surface.Fundação para a Ciência e a Tecnologia (FCT) - Project CHEMBIO – POCI/BIO/61872/2004; SFRH/BD/ 31661/2006; SFRH/BPD/20582/2004.ElsevierUniversidade do MinhoSimões, M.Simões, Lúcia C.Cleto, S.Pereira, Maria OlíviaVieira, M. J.2008-022008-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7734eng"International Journal of Food Microbiology". ISSN 0168-1605. 121:3 (2008) 335-341.0168-160510.1016/j.ijfoodmicro.2007.11.04118155793www.elsevier.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:31:45Zoai:repositorium.sdum.uminho.pt:1822/7734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:27:02.447586Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
title |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
spellingShingle |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces Simões, M. Biofilms Cetyltrimethyl ammonium bromide Detachment Disinfection Ortho-phthalaldehyde Pseudomonas fluorescens ortho-phthaialdehyde Science & Technology |
title_short |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
title_full |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
title_fullStr |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
title_full_unstemmed |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
title_sort |
The effects of a biocide and a surfactant on the detachment of Pseudomonas fluorescens from glass surfaces |
author |
Simões, M. |
author_facet |
Simões, M. Simões, Lúcia C. Cleto, S. Pereira, Maria Olívia Vieira, M. J. |
author_role |
author |
author2 |
Simões, Lúcia C. Cleto, S. Pereira, Maria Olívia Vieira, M. J. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Simões, M. Simões, Lúcia C. Cleto, S. Pereira, Maria Olívia Vieira, M. J. |
dc.subject.por.fl_str_mv |
Biofilms Cetyltrimethyl ammonium bromide Detachment Disinfection Ortho-phthalaldehyde Pseudomonas fluorescens ortho-phthaialdehyde Science & Technology |
topic |
Biofilms Cetyltrimethyl ammonium bromide Detachment Disinfection Ortho-phthalaldehyde Pseudomonas fluorescens ortho-phthaialdehyde Science & Technology |
description |
Application of antimicrobial chemicals is a general procedure in the cleaning and disinfection of food-contacting surfaces. Adhesion to glass surfaces and chemically induced detachment of Pseudomonas fluorescens ATCC 13525T were studied in situ, under flow conditions, in a well-controlled parallel plate flow chamber (PPFC). Ortho-phthalaldehyde (OPA) and cetyltrimethyl ammonium bromide (CTAB) were applied separately, at several concentrations, to attached bacteria and their subsequent detachment was monitored. Following treatments the remaining adhered bacteria were characterized in terms of viability and cell size. Simultaneously, the planktonic cell surface was characterized in order to correlate PPFC results with thermodynamic approaches for adhesion evaluation, and surface free energy of chemically treated cells with adhesion strength. About 2.8 × 106 cells/cm2 adhered to the glass surface after 30 min of bacterial flow, although thermodynamic analyses evidenced unfavourable adhesion. The independent application of OPA and CTAB promoted bacterial detachment to a small extent (16% of total cells). The remaining adhering bacteria were totally non-viable for OPA ≥ 0.75 mM and CTAB ≥ 0.25 mM, showing a lack of correlation between bacterial viability and detachment. The cellular size decreased as attachment proceeded and with chemical treatment. Both chemicals altered the cell surface properties, increasing the cell-glass adhesion strength, and promoting the emergence of polar characteristics. The overall results emphasize that OPA and CTAB were markedly ineffective in removing glass-attached P. fluorescens, demonstrating that bacteria can be non-viable but remain strongly attached to the adhesion surface. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-02 2008-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/7734 |
url |
http://hdl.handle.net/1822/7734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"International Journal of Food Microbiology". ISSN 0168-1605. 121:3 (2008) 335-341. 0168-1605 10.1016/j.ijfoodmicro.2007.11.041 18155793 www.elsevier.com |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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