Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/147025 |
Resumo: | LP was granted a Ph.D. fellowship by Fundacao para a Ciencia e a Tecnologia and European Social Fund (SFRH/BD/81307/2011). This work was supported by the Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). Also, this work is a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020-Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundacao para a Ciencia e a Tecnologia (FCT). |
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Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coliAntimicrobial resistanceBacteriaGenomicsProteomicsPublic healthTranscriptomicsMicrobiologyMicrobiology (medical)SDG 3 - Good Health and Well-beingLP was granted a Ph.D. fellowship by Fundacao para a Ciencia e a Tecnologia and European Social Fund (SFRH/BD/81307/2011). This work was supported by the Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). Also, this work is a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020-Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundacao para a Ciencia e a Tecnologia (FCT).Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strain C999 was isolated of a Spanish patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug-resistance phenotype and carried beta-lactamase genes encoding CTX-M-15, TEM-1, and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of C999 were obtained in this work by two-dimensional gel electrophoresis (2DE) followed by fingerprint sequencing through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). A total of 602 proteins were identified in the different cell fractions, several of which are related to stress response systems, cellular responses, and antibiotic and drug responses, consistent with the multidrug-resistance phenotype. In parallel, whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) was done to identify and quantify the genes present and expressing. The in silico prediction following WGS confirmed our strain as being serotype O25:H4 and sequence type ST131. The presence of proteins related to antibiotic resistance and virulence in an O25:H4-ST131 E. coli clone are serious indicators of the continued threat of antibiotic resistance spread amongst healthcare institutions. On a positive note, a multiomics approach can facilitate surveillance and more detailed characterization of virulent bacterial clones from hospital environments.LAQV@REQUIMTERUNPinto, LuísTorres, CarmenGil, ConchaNunes-Miranda, Júlio D.Santos, Hugo M.Borges, VítorGomes, João P.Silva, CatarinaVieira, LuísPereira, José E.Poeta, PatríciaIgrejas, Gilberto2023-01-05T22:11:32Z2019-05-032019-05-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147025eng1664-302XPURE: 14288481https://doi.org/10.3389/fmicb.2019.00831info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:00Zoai:run.unl.pt:10362/147025Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:45.166039Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| title |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| spellingShingle |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli Pinto, Luís Antimicrobial resistance Bacteria Genomics Proteomics Public health Transcriptomics Microbiology Microbiology (medical) SDG 3 - Good Health and Well-being |
| title_short |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| title_full |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| title_fullStr |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| title_full_unstemmed |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| title_sort |
Multiomics assessment of gene expression in a clinical strain of CTX-M-15-producing ST131 Escherichia coli |
| author |
Pinto, Luís |
| author_facet |
Pinto, Luís Torres, Carmen Gil, Concha Nunes-Miranda, Júlio D. Santos, Hugo M. Borges, Vítor Gomes, João P. Silva, Catarina Vieira, Luís Pereira, José E. Poeta, Patrícia Igrejas, Gilberto |
| author_role |
author |
| author2 |
Torres, Carmen Gil, Concha Nunes-Miranda, Júlio D. Santos, Hugo M. Borges, Vítor Gomes, João P. Silva, Catarina Vieira, Luís Pereira, José E. Poeta, Patrícia Igrejas, Gilberto |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE RUN |
| dc.contributor.author.fl_str_mv |
Pinto, Luís Torres, Carmen Gil, Concha Nunes-Miranda, Júlio D. Santos, Hugo M. Borges, Vítor Gomes, João P. Silva, Catarina Vieira, Luís Pereira, José E. Poeta, Patrícia Igrejas, Gilberto |
| dc.subject.por.fl_str_mv |
Antimicrobial resistance Bacteria Genomics Proteomics Public health Transcriptomics Microbiology Microbiology (medical) SDG 3 - Good Health and Well-being |
| topic |
Antimicrobial resistance Bacteria Genomics Proteomics Public health Transcriptomics Microbiology Microbiology (medical) SDG 3 - Good Health and Well-being |
| description |
LP was granted a Ph.D. fellowship by Fundacao para a Ciencia e a Tecnologia and European Social Fund (SFRH/BD/81307/2011). This work was supported by the Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). Also, this work is a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020-Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundacao para a Ciencia e a Tecnologia (FCT). |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-05-03 2019-05-03T00:00:00Z 2023-01-05T22:11:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/147025 |
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http://hdl.handle.net/10362/147025 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1664-302X PURE: 14288481 https://doi.org/10.3389/fmicb.2019.00831 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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