Amperometric enzyme sensor for the rapid determination of histamine
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/16106 |
Resumo: | The concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, -0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides an useful linear range (between 1 and 75 mg L-1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Amperometric enzyme sensor for the rapid determination of histamineFishHistamineScreen-printed carbon electrodeEnzymatic sensorThe concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, -0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides an useful linear range (between 1 and 75 mg L-1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%.The authors are grateful for the financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 through the project UID/ QUI/50006/2013. The authors would also like to thank the EU and FCT for funding through the projects: FishBioSensing - Portable electrochemical (bio)sensing devices for safety and quality assessment of fishery products (02/SAICT/2016, POCI-01-0145-FEDER-023817) and FOODnanoHEALTH - Qualidade e Segurança Alimentar – uma abordagem (nano)tecnológica (Portugal2020, Norte-01-0145-FEDER-000011).RSCRepositório Científico do Instituto Politécnico do PortoTorre, RicardaCosta Rama, EstefaníaLOPES, PaulaNouws, HenriDelerue-Matos, Cristina2020-07-10T14:39:20Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/16106eng10.1039/C8AY02610Finfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:02:12Zoai:recipp.ipp.pt:10400.22/16106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:35:49.156639Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Amperometric enzyme sensor for the rapid determination of histamine |
title |
Amperometric enzyme sensor for the rapid determination of histamine |
spellingShingle |
Amperometric enzyme sensor for the rapid determination of histamine Torre, Ricarda Fish Histamine Screen-printed carbon electrode Enzymatic sensor |
title_short |
Amperometric enzyme sensor for the rapid determination of histamine |
title_full |
Amperometric enzyme sensor for the rapid determination of histamine |
title_fullStr |
Amperometric enzyme sensor for the rapid determination of histamine |
title_full_unstemmed |
Amperometric enzyme sensor for the rapid determination of histamine |
title_sort |
Amperometric enzyme sensor for the rapid determination of histamine |
author |
Torre, Ricarda |
author_facet |
Torre, Ricarda Costa Rama, Estefanía LOPES, Paula Nouws, Henri Delerue-Matos, Cristina |
author_role |
author |
author2 |
Costa Rama, Estefanía LOPES, Paula Nouws, Henri Delerue-Matos, Cristina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Torre, Ricarda Costa Rama, Estefanía LOPES, Paula Nouws, Henri Delerue-Matos, Cristina |
dc.subject.por.fl_str_mv |
Fish Histamine Screen-printed carbon electrode Enzymatic sensor |
topic |
Fish Histamine Screen-printed carbon electrode Enzymatic sensor |
description |
The concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, -0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides an useful linear range (between 1 and 75 mg L-1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2020-07-10T14:39:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/16106 |
url |
http://hdl.handle.net/10400.22/16106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1039/C8AY02610F |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
RSC |
publisher.none.fl_str_mv |
RSC |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131449007276032 |