The habenula as a critical node in chronic stress-related anxiety
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/51557 |
Resumo: | The habenula is activated in response to stressful and aversive events, resulting in exploratory inhibition. Although possible mechanisms for habenula activation have been proposed, the effects of chronic stress on the habenular structure have never been studied. Herein, we assessed changes in volume, cell density and dendritic structure of habenular cells after chronic stress exposure using stereological and 3D morphological analysis. This study shows for the first time that there is a hemispherical asymmetry in the medial habenula (MHb) of the adult rat, with the right MHb containing more neurons than its left counterpart. Additionally, it shows that chronic stress induces a bilateral atrophy of both the MHb and the lateral habenula (LHb). This atrophy was accompanied by a reduction of the number of neurons in the right MHb and the number of glial cells in the bilateral LHb, but not by changes in the dendritic arbors of multipolar neurons. Importantly, these structural changes were correlated with elevated levels of serum corticosterone and increased anxious-like behavior in stressed animals. To further assess the role of the habenula in stress-related anxiety, bilateral lesions of the LHb were performed; interestingly, in lesioned animals the chronic stress protocol did not trigger increases in circulating corticosterone or anxious-like behavior. This study highlights the role of the habenula in the stress responses and how its sub-regions are structurally impacted by chronic stress with physiological and behavioral consequences. |
id |
RCAP_b0a4ad70f5b4c8a1cda058b07dd97b4a |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/51557 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The habenula as a critical node in chronic stress-related anxietyHabenulaStressAnxietyStereologyCorticosteroneCiências Médicas::Medicina ClínicaScience & TechnologyThe habenula is activated in response to stressful and aversive events, resulting in exploratory inhibition. Although possible mechanisms for habenula activation have been proposed, the effects of chronic stress on the habenular structure have never been studied. Herein, we assessed changes in volume, cell density and dendritic structure of habenular cells after chronic stress exposure using stereological and 3D morphological analysis. This study shows for the first time that there is a hemispherical asymmetry in the medial habenula (MHb) of the adult rat, with the right MHb containing more neurons than its left counterpart. Additionally, it shows that chronic stress induces a bilateral atrophy of both the MHb and the lateral habenula (LHb). This atrophy was accompanied by a reduction of the number of neurons in the right MHb and the number of glial cells in the bilateral LHb, but not by changes in the dendritic arbors of multipolar neurons. Importantly, these structural changes were correlated with elevated levels of serum corticosterone and increased anxious-like behavior in stressed animals. To further assess the role of the habenula in stress-related anxiety, bilateral lesions of the LHb were performed; interestingly, in lesioned animals the chronic stress protocol did not trigger increases in circulating corticosterone or anxious-like behavior. This study highlights the role of the habenula in the stress responses and how its sub-regions are structurally impacted by chronic stress with physiological and behavioral consequences.Prof. Sharif Taha formerly of University of Utah for providing training and technical expertise on habenula research; Ana Lima and Mónica Dias for histological processing of samples; and Diana Afonso and Ana Veloso for help with the stress protocol. LRJ was supported by fellowships: UMINHO/BPD/27/2013 funded by CCDR-N and Programa Operacional Região Norte (ON.2) from QREN/FEDER; 2014/CON3/CAN23 from Fundação Luso-Americana; and UMINHO/BPD/63/2015 from Fundação Calouste Gulbenkian funded project (contract grant number P-139977). AN was supported by a fellowship from project ANR/NEU-OSD/0258/2012 funded by Fundação para a Ciência e Tecnologia (FCT) and Agence Nationale de la Recherche (ANR). Financial support for this work was provided by FEDER funds through the Operational Programme Competitiveness Factors - COMPETE and National Funds through FCT - Foundation for Science and Technology under the project POCI-01-0145-FEDER-007038; and by the project NORTE-01-0145-FEDER-000013, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF)info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoJacinto, Luís Ricardo MonteiroMata, RuiNovais, Ashley CruzMarques, FernandaSousa, Nuno2017-03-072017-03-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51557engJacinto, L. R., Mata, R., Novais, A., Marques, F., & Sousa, N. (2017). The habenula as a critical node in chronic stress-related anxiety. Experimental neurology, 289, 46-540014-488610.1016/j.expneurol.2016.12.00327940019https://www.sciencedirect.com/science/article/pii/S0014488616304034info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:11Zoai:repositorium.sdum.uminho.pt:1822/51557Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:25.764030Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The habenula as a critical node in chronic stress-related anxiety |
title |
The habenula as a critical node in chronic stress-related anxiety |
spellingShingle |
The habenula as a critical node in chronic stress-related anxiety Jacinto, Luís Ricardo Monteiro Habenula Stress Anxiety Stereology Corticosterone Ciências Médicas::Medicina Clínica Science & Technology |
title_short |
The habenula as a critical node in chronic stress-related anxiety |
title_full |
The habenula as a critical node in chronic stress-related anxiety |
title_fullStr |
The habenula as a critical node in chronic stress-related anxiety |
title_full_unstemmed |
The habenula as a critical node in chronic stress-related anxiety |
title_sort |
The habenula as a critical node in chronic stress-related anxiety |
author |
Jacinto, Luís Ricardo Monteiro |
author_facet |
Jacinto, Luís Ricardo Monteiro Mata, Rui Novais, Ashley Cruz Marques, Fernanda Sousa, Nuno |
author_role |
author |
author2 |
Mata, Rui Novais, Ashley Cruz Marques, Fernanda Sousa, Nuno |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Jacinto, Luís Ricardo Monteiro Mata, Rui Novais, Ashley Cruz Marques, Fernanda Sousa, Nuno |
dc.subject.por.fl_str_mv |
Habenula Stress Anxiety Stereology Corticosterone Ciências Médicas::Medicina Clínica Science & Technology |
topic |
Habenula Stress Anxiety Stereology Corticosterone Ciências Médicas::Medicina Clínica Science & Technology |
description |
The habenula is activated in response to stressful and aversive events, resulting in exploratory inhibition. Although possible mechanisms for habenula activation have been proposed, the effects of chronic stress on the habenular structure have never been studied. Herein, we assessed changes in volume, cell density and dendritic structure of habenular cells after chronic stress exposure using stereological and 3D morphological analysis. This study shows for the first time that there is a hemispherical asymmetry in the medial habenula (MHb) of the adult rat, with the right MHb containing more neurons than its left counterpart. Additionally, it shows that chronic stress induces a bilateral atrophy of both the MHb and the lateral habenula (LHb). This atrophy was accompanied by a reduction of the number of neurons in the right MHb and the number of glial cells in the bilateral LHb, but not by changes in the dendritic arbors of multipolar neurons. Importantly, these structural changes were correlated with elevated levels of serum corticosterone and increased anxious-like behavior in stressed animals. To further assess the role of the habenula in stress-related anxiety, bilateral lesions of the LHb were performed; interestingly, in lesioned animals the chronic stress protocol did not trigger increases in circulating corticosterone or anxious-like behavior. This study highlights the role of the habenula in the stress responses and how its sub-regions are structurally impacted by chronic stress with physiological and behavioral consequences. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-07 2017-03-07T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51557 |
url |
http://hdl.handle.net/1822/51557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jacinto, L. R., Mata, R., Novais, A., Marques, F., & Sousa, N. (2017). The habenula as a critical node in chronic stress-related anxiety. Experimental neurology, 289, 46-54 0014-4886 10.1016/j.expneurol.2016.12.003 27940019 https://www.sciencedirect.com/science/article/pii/S0014488616304034 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132851680051200 |