The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/21901 |
Resumo: | Nannochloropsis oceanica is a microalga, highly concentrated in protein and EPA (eicosapentaenoic acid). It has a recalcitrant cell wall that decreases nutrient digestibility. The objective of this study is to evaluate the effect of Nannochloropsis oceanica feed inclusion on the muscle proteome of fattening rabbits. Twenty rabbits were housed in individual cages for 5 weeks and were fed a control diet (n = 10) and one with 4.45% inclusion of microalga, replacing whole soybean meal (n = 10). After slaughter, samples of gastrocnemius muscle were taken for label-free proteomic analysis. A total of 1497 proteins were identified, 46 with differential abundance. Control rabbits had high abundance of proteins related to protein metabolism, suggesting higher muscle protein turnover. They also had higher abundance of structural proteins, suggesting a less tender meat by comparison with algae-fed rabbits. These had high abundance of proteins related to amino acid catabolism (Phe, Tyr) and synthesis (Gln). In addition, they had high abundance of proteins related to protein breakdown, overall suggesting that more tender meat may result from algae feeding |
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The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteomeNannochloropsis oceanicafeedrabbitNannochloropsis oceanica is a microalga, highly concentrated in protein and EPA (eicosapentaenoic acid). It has a recalcitrant cell wall that decreases nutrient digestibility. The objective of this study is to evaluate the effect of Nannochloropsis oceanica feed inclusion on the muscle proteome of fattening rabbits. Twenty rabbits were housed in individual cages for 5 weeks and were fed a control diet (n = 10) and one with 4.45% inclusion of microalga, replacing whole soybean meal (n = 10). After slaughter, samples of gastrocnemius muscle were taken for label-free proteomic analysis. A total of 1497 proteins were identified, 46 with differential abundance. Control rabbits had high abundance of proteins related to protein metabolism, suggesting higher muscle protein turnover. They also had higher abundance of structural proteins, suggesting a less tender meat by comparison with algae-fed rabbits. These had high abundance of proteins related to amino acid catabolism (Phe, Tyr) and synthesis (Gln). In addition, they had high abundance of proteins related to protein breakdown, overall suggesting that more tender meat may result from algae feedingRepositório da Universidade de LisboaRibeiro, D.M.Bandarrinha, J.Nanni, P.Alves, S.P.Martins, C.F.Bessa, R.J.B.Falcão-e-Cunha, L.Almeida, A.M.2021-09-22T09:59:33Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21901engRibeiro D.M., Bandarrinha J., Nanni P., Alves S.P., Martins C.F., Bessa R.J.B., Falcão-e-Cunha L., Almeida A.M. 2020. The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome. Journal of Proteomics 222: 103783https://doi.org/10.1016/j.jprot.2020.103783info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:27Zoai:www.repository.utl.pt:10400.5/21901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:26.441579Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
title |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
spellingShingle |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome Ribeiro, D.M. Nannochloropsis oceanica feed rabbit |
title_short |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
title_full |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
title_fullStr |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
title_full_unstemmed |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
title_sort |
The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome |
author |
Ribeiro, D.M. |
author_facet |
Ribeiro, D.M. Bandarrinha, J. Nanni, P. Alves, S.P. Martins, C.F. Bessa, R.J.B. Falcão-e-Cunha, L. Almeida, A.M. |
author_role |
author |
author2 |
Bandarrinha, J. Nanni, P. Alves, S.P. Martins, C.F. Bessa, R.J.B. Falcão-e-Cunha, L. Almeida, A.M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Ribeiro, D.M. Bandarrinha, J. Nanni, P. Alves, S.P. Martins, C.F. Bessa, R.J.B. Falcão-e-Cunha, L. Almeida, A.M. |
dc.subject.por.fl_str_mv |
Nannochloropsis oceanica feed rabbit |
topic |
Nannochloropsis oceanica feed rabbit |
description |
Nannochloropsis oceanica is a microalga, highly concentrated in protein and EPA (eicosapentaenoic acid). It has a recalcitrant cell wall that decreases nutrient digestibility. The objective of this study is to evaluate the effect of Nannochloropsis oceanica feed inclusion on the muscle proteome of fattening rabbits. Twenty rabbits were housed in individual cages for 5 weeks and were fed a control diet (n = 10) and one with 4.45% inclusion of microalga, replacing whole soybean meal (n = 10). After slaughter, samples of gastrocnemius muscle were taken for label-free proteomic analysis. A total of 1497 proteins were identified, 46 with differential abundance. Control rabbits had high abundance of proteins related to protein metabolism, suggesting higher muscle protein turnover. They also had higher abundance of structural proteins, suggesting a less tender meat by comparison with algae-fed rabbits. These had high abundance of proteins related to amino acid catabolism (Phe, Tyr) and synthesis (Gln). In addition, they had high abundance of proteins related to protein breakdown, overall suggesting that more tender meat may result from algae feeding |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-09-22T09:59:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/21901 |
url |
http://hdl.handle.net/10400.5/21901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro D.M., Bandarrinha J., Nanni P., Alves S.P., Martins C.F., Bessa R.J.B., Falcão-e-Cunha L., Almeida A.M. 2020. The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome. Journal of Proteomics 222: 103783 https://doi.org/10.1016/j.jprot.2020.103783 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131156923285504 |