NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Bispo, Daniela S. C.
Data de Publicação: 2022
Outros Autores: Jesus, Catarina S. H., Correia, Marlene, Ferreira, Filipa, Bonifazio, Giulia, Goodfellow, Brian J., Oliveira, Mariana B., Mano, João F., Gil, Ana M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/35629
Resumo: This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics may unveil novel metabolic information on the osteogenic differentiation of MSCs, allowing their behavior to be understood and monitored/guided toward effective therapies. Our results unveiled statistically relevant changes in the levels of just over 30 identified metabolites, illustrating a highly dynamic process with significant variations throughout the whole 21-day period of osteogenic differentiation, mainly involving amino acid metabolism and protein synthesis; energy metabolism and the roles of glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation; cell membrane metabolism; nucleotide metabolism (including the specific involvement of O-glycosylation intermediates and NAD+); and metabolic players in protective antioxidative mechanisms (such as glutathione and specific amino acids). Different metabolic stages are proposed and are supported by putative biochemical explanations for the metabolite changes observed. This work lays the groundwork for the use of untargeted NMR metabolomics to find potential metabolic markers of osteogenic differentiation efficac
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spelling NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cellsStem cellsDifferentiationOsteogenic differentiationOsteogenesisMetabolic switchNMRMetabolomicsMetabonomicsThis Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics may unveil novel metabolic information on the osteogenic differentiation of MSCs, allowing their behavior to be understood and monitored/guided toward effective therapies. Our results unveiled statistically relevant changes in the levels of just over 30 identified metabolites, illustrating a highly dynamic process with significant variations throughout the whole 21-day period of osteogenic differentiation, mainly involving amino acid metabolism and protein synthesis; energy metabolism and the roles of glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation; cell membrane metabolism; nucleotide metabolism (including the specific involvement of O-glycosylation intermediates and NAD+); and metabolic players in protective antioxidative mechanisms (such as glutathione and specific amino acids). Different metabolic stages are proposed and are supported by putative biochemical explanations for the metabolite changes observed. This work lays the groundwork for the use of untargeted NMR metabolomics to find potential metabolic markers of osteogenic differentiation efficacAmerican Chemical Society2023-01-05T12:42:16Z2022-03-04T00:00:00Z2022-03-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/35629eng1535-389310.1021/acs.jproteome.1c00832Bispo, Daniela S. C.Jesus, Catarina S. H.Correia, MarleneFerreira, FilipaBonifazio, GiuliaGoodfellow, Brian J.Oliveira, Mariana B.Mano, João F.Gil, Ana M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:09:01Zoai:ria.ua.pt:10773/35629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:06:45.855243Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
title NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
spellingShingle NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
Bispo, Daniela S. C.
Stem cells
Differentiation
Osteogenic differentiation
Osteogenesis
Metabolic switch
NMR
Metabolomics
Metabonomics
title_short NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
title_full NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
title_fullStr NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
title_full_unstemmed NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
title_sort NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-derived mesenchymal stem cells
author Bispo, Daniela S. C.
author_facet Bispo, Daniela S. C.
Jesus, Catarina S. H.
Correia, Marlene
Ferreira, Filipa
Bonifazio, Giulia
Goodfellow, Brian J.
Oliveira, Mariana B.
Mano, João F.
Gil, Ana M.
author_role author
author2 Jesus, Catarina S. H.
Correia, Marlene
Ferreira, Filipa
Bonifazio, Giulia
Goodfellow, Brian J.
Oliveira, Mariana B.
Mano, João F.
Gil, Ana M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bispo, Daniela S. C.
Jesus, Catarina S. H.
Correia, Marlene
Ferreira, Filipa
Bonifazio, Giulia
Goodfellow, Brian J.
Oliveira, Mariana B.
Mano, João F.
Gil, Ana M.
dc.subject.por.fl_str_mv Stem cells
Differentiation
Osteogenic differentiation
Osteogenesis
Metabolic switch
NMR
Metabolomics
Metabonomics
topic Stem cells
Differentiation
Osteogenic differentiation
Osteogenesis
Metabolic switch
NMR
Metabolomics
Metabonomics
description This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics may unveil novel metabolic information on the osteogenic differentiation of MSCs, allowing their behavior to be understood and monitored/guided toward effective therapies. Our results unveiled statistically relevant changes in the levels of just over 30 identified metabolites, illustrating a highly dynamic process with significant variations throughout the whole 21-day period of osteogenic differentiation, mainly involving amino acid metabolism and protein synthesis; energy metabolism and the roles of glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation; cell membrane metabolism; nucleotide metabolism (including the specific involvement of O-glycosylation intermediates and NAD+); and metabolic players in protective antioxidative mechanisms (such as glutathione and specific amino acids). Different metabolic stages are proposed and are supported by putative biochemical explanations for the metabolite changes observed. This work lays the groundwork for the use of untargeted NMR metabolomics to find potential metabolic markers of osteogenic differentiation efficac
publishDate 2022
dc.date.none.fl_str_mv 2022-03-04T00:00:00Z
2022-03-04
2023-01-05T12:42:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/35629
url http://hdl.handle.net/10773/35629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1535-3893
10.1021/acs.jproteome.1c00832
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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