Quantificação de DNA plasmático em pacientes com cancro da mama
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/22401 |
Resumo: | Breast cancer is the leading cause of cancer death in women worldwide. The purpose of our study was to determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR based DNA quantification methodology and determine the kinetics of circulating plasma DNA in surgically treated patients. Our standard protocol for quantification of cell free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. The quantification was perfomed by real-time PCR amplification of the human telomerase reverse transcriptase gene (hTERT). We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs 77.06 ng/ml, p<0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery (105.2 vs 59.0 ng/ml, p=0.001). Increased plasma cell free DNA concentration was a strong risk factor for the presence of breast cancer, conferring an increased risk for the development of this disease (OR, 12.32; 95% CI, 2.09 52.28; p<0.001). High levels of plasma DNA were also correlated with a decrease in patients overall survival. There were no association between clinicopathological parameters and concentrations of cell free circulating DNA. In conclusion, cell-free DNA is significantly increased in plasma of breast cancer patients, which is associated with an increased risk for the presence of this disease and decrease of patient s survival. Therefore, quantification of circulating DNA by real-time PCR may be a good and simple tool for early detection of breast cancer with potential to clinical applicability together with other current methods used for monitoring the disease. |
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Quantificação de DNA plasmático em pacientes com cancro da mamaOncologia MolecularPortoBreast cancer is the leading cause of cancer death in women worldwide. The purpose of our study was to determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR based DNA quantification methodology and determine the kinetics of circulating plasma DNA in surgically treated patients. Our standard protocol for quantification of cell free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. The quantification was perfomed by real-time PCR amplification of the human telomerase reverse transcriptase gene (hTERT). We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs 77.06 ng/ml, p<0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery (105.2 vs 59.0 ng/ml, p=0.001). Increased plasma cell free DNA concentration was a strong risk factor for the presence of breast cancer, conferring an increased risk for the development of this disease (OR, 12.32; 95% CI, 2.09 52.28; p<0.001). High levels of plasma DNA were also correlated with a decrease in patients overall survival. There were no association between clinicopathological parameters and concentrations of cell free circulating DNA. In conclusion, cell-free DNA is significantly increased in plasma of breast cancer patients, which is associated with an increased risk for the presence of this disease and decrease of patient s survival. Therefore, quantification of circulating DNA by real-time PCR may be a good and simple tool for early detection of breast cancer with potential to clinical applicability together with other current methods used for monitoring the disease.Faculdade de Medicina da Universidade do PortoFMUP20062011-02-07T00:00:00Z2011-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/mswordapplication/mswordapplication/mswordapplication/mswordapplication/mswordapplication/mswordapplication/mswordhttp://hdl.handle.net/10216/22401porFerreira, Maria Manuel da Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:56:46Zoai:repositorio-aberto.up.pt:10216/22401Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:35:39.886331Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Quantificação de DNA plasmático em pacientes com cancro da mama |
title |
Quantificação de DNA plasmático em pacientes com cancro da mama |
spellingShingle |
Quantificação de DNA plasmático em pacientes com cancro da mama Ferreira, Maria Manuel da Silva Oncologia Molecular Porto |
title_short |
Quantificação de DNA plasmático em pacientes com cancro da mama |
title_full |
Quantificação de DNA plasmático em pacientes com cancro da mama |
title_fullStr |
Quantificação de DNA plasmático em pacientes com cancro da mama |
title_full_unstemmed |
Quantificação de DNA plasmático em pacientes com cancro da mama |
title_sort |
Quantificação de DNA plasmático em pacientes com cancro da mama |
author |
Ferreira, Maria Manuel da Silva |
author_facet |
Ferreira, Maria Manuel da Silva |
author_role |
author |
dc.contributor.author.fl_str_mv |
Ferreira, Maria Manuel da Silva |
dc.subject.por.fl_str_mv |
Oncologia Molecular Porto |
topic |
Oncologia Molecular Porto |
description |
Breast cancer is the leading cause of cancer death in women worldwide. The purpose of our study was to determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR based DNA quantification methodology and determine the kinetics of circulating plasma DNA in surgically treated patients. Our standard protocol for quantification of cell free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. The quantification was perfomed by real-time PCR amplification of the human telomerase reverse transcriptase gene (hTERT). We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs 77.06 ng/ml, p<0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery (105.2 vs 59.0 ng/ml, p=0.001). Increased plasma cell free DNA concentration was a strong risk factor for the presence of breast cancer, conferring an increased risk for the development of this disease (OR, 12.32; 95% CI, 2.09 52.28; p<0.001). High levels of plasma DNA were also correlated with a decrease in patients overall survival. There were no association between clinicopathological parameters and concentrations of cell free circulating DNA. In conclusion, cell-free DNA is significantly increased in plasma of breast cancer patients, which is associated with an increased risk for the presence of this disease and decrease of patient s survival. Therefore, quantification of circulating DNA by real-time PCR may be a good and simple tool for early detection of breast cancer with potential to clinical applicability together with other current methods used for monitoring the disease. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2011-02-07T00:00:00Z 2011-02-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/22401 |
url |
http://hdl.handle.net/10216/22401 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/msword application/msword application/msword application/msword application/msword application/msword application/msword |
dc.publisher.none.fl_str_mv |
Faculdade de Medicina da Universidade do Porto FMUP |
publisher.none.fl_str_mv |
Faculdade de Medicina da Universidade do Porto FMUP |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136265570877440 |