Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485

Detalhes bibliográficos
Autor(a) principal: Sales, Amanda Emmanuelle
Data de Publicação: 2013
Outros Autores: Souza, Fabiana América Silva Dantas de, Teixeira, J. A., Porto, Tatiana Souza, Porto, Ana L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25532
Resumo: Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K < 1), with an optimum activity of 835 U ml−1 in the bottom phase (salt-rich phase). The best conditions for extractive fermentation were obtained with 18 % PEG 8000 and 13 % Na2SO4. Characterization showed that it is a metalloprotease, as a strong inhibition—residual activity of 3.13 %—occurred in the presence of ethylenediaminetetraacetic acid. It was also observed that enzymatic activity was stimulated in the presence of ions: CaCl2 (440 %), MgCl2 (440 %), FeSO4 (268 %), and KCl (268 %). The obtained results indicate that the use of a low-cost substrate and the integration of fermentation with an aqueous two-phase system extraction may be an interesting alternative for the production of fibrinolytic protease.
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spelling Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485Fibrinolytic proteaseBacillusPEG/sodium sulfateATPSExtractive fermentationScience & TechnologyFibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K < 1), with an optimum activity of 835 U ml−1 in the bottom phase (salt-rich phase). The best conditions for extractive fermentation were obtained with 18 % PEG 8000 and 13 % Na2SO4. Characterization showed that it is a metalloprotease, as a strong inhibition—residual activity of 3.13 %—occurred in the presence of ethylenediaminetetraacetic acid. It was also observed that enzymatic activity was stimulated in the presence of ions: CaCl2 (440 %), MgCl2 (440 %), FeSO4 (268 %), and KCl (268 %). The obtained results indicate that the use of a low-cost substrate and the integration of fermentation with an aqueous two-phase system extraction may be an interesting alternative for the production of fibrinolytic protease.The authors thank CAPES (National Council for the Improvement of Higher Education) for the scholarship and CNPq (National Counsel of Technological and Scientific Development) and RENORBIO for the financial support.SpringerUniversidade do MinhoSales, Amanda EmmanuelleSouza, Fabiana América Silva Dantas deTeixeira, J. A.Porto, Tatiana SouzaPorto, Ana L. F.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25532eng0273-22890273-228910.1007/s12010-013-0306-z23716141info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:20Zoai:repositorium.sdum.uminho.pt:1822/25532Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:38:18.244456Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
title Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
spellingShingle Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
Sales, Amanda Emmanuelle
Fibrinolytic protease
Bacillus
PEG/sodium sulfate
ATPS
Extractive fermentation
Science & Technology
title_short Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
title_full Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
title_fullStr Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
title_full_unstemmed Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
title_sort Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485
author Sales, Amanda Emmanuelle
author_facet Sales, Amanda Emmanuelle
Souza, Fabiana América Silva Dantas de
Teixeira, J. A.
Porto, Tatiana Souza
Porto, Ana L. F.
author_role author
author2 Souza, Fabiana América Silva Dantas de
Teixeira, J. A.
Porto, Tatiana Souza
Porto, Ana L. F.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sales, Amanda Emmanuelle
Souza, Fabiana América Silva Dantas de
Teixeira, J. A.
Porto, Tatiana Souza
Porto, Ana L. F.
dc.subject.por.fl_str_mv Fibrinolytic protease
Bacillus
PEG/sodium sulfate
ATPS
Extractive fermentation
Science & Technology
topic Fibrinolytic protease
Bacillus
PEG/sodium sulfate
ATPS
Extractive fermentation
Science & Technology
description Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K < 1), with an optimum activity of 835 U ml−1 in the bottom phase (salt-rich phase). The best conditions for extractive fermentation were obtained with 18 % PEG 8000 and 13 % Na2SO4. Characterization showed that it is a metalloprotease, as a strong inhibition—residual activity of 3.13 %—occurred in the presence of ethylenediaminetetraacetic acid. It was also observed that enzymatic activity was stimulated in the presence of ions: CaCl2 (440 %), MgCl2 (440 %), FeSO4 (268 %), and KCl (268 %). The obtained results indicate that the use of a low-cost substrate and the integration of fermentation with an aqueous two-phase system extraction may be an interesting alternative for the production of fibrinolytic protease.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
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0273-2289
10.1007/s12010-013-0306-z
23716141
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