The relation of HPV infection and expression of p53 and p16 proteins in esophageal squamous cells carcinoma

• Autor(a) principal: Pastrez, Paula Roberta Aguiar
• Data de Publicação: 2017
• Outros Autores: Mariano, Vânia Sammartino, Costa, Allini Mafra da, Silva, Estela Maria, Scapulatempo-Neto, Cristovam, Guimarães, Denise Peixoto, Fava, Gilberto, Neto, Said Abdala Zemi, Nunes, Emily Montosa, Longatto Filho, Adhemar, et. al.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/49111
• topic: Esophageal neoplasias; p16; p53; Immunohistochemistry; HPV; Ciências Médicas::Medicina Básica; Science & Technology

GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.