Self-buffering and biocompatible ionic liquid based biological media for enzymatic research
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/20570 |
Resumo: | In the present work, we have designed five new biocompatible, self-buffering ionic liquids (ILs) in which the cationic part is derived from conventional tetra-butylphosphonium (TBP) and the anionic part is derived from common biological buffers TAPS, MOPS, EPPS, CAPS, and BICINE. The new ionic liquid based biocompatible media ([TBP][TAPS], [TBP][MOPS], [TBP][EPPS], [TBP][CAPS], and [TBP][BICINE]) were found to be suitable for overcoming most of the problems associated with enzymatic research including optimum pH range, biocompatibility, and extraction. In comparison with the conventional ionic liquid based biological media, these new media do not involve an external buffering compound and maintain the optimum pH by their self-buffering capability. The buffering nature of these new ILs was confirmed by measuring their pH profiles and protonation constants in aqueous solutions at different temperatures. The biocompatibility of these new ILs was also confirmed by measuring the biological activity of the enzyme alpha-chymotrypsin (alpha-CT) in the aqueous media of these ILs. Moreover, these new ionic liquids can form an aqueous two phase system (ATPS) with a common inorganic salt such as sodium sulfate. The excellent extraction efficiency (100%) of these ionic liquid-based ATPS was observed for the extraction of enzyme alpha-CT, in its active form, in an IL-rich phase via a single stage extraction. Since the selected common biological buffers are biocompatible and nontoxic compounds, the ionic liquids derived from these buffer compounds could be more promising for biological research. |
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Self-buffering and biocompatible ionic liquid based biological media for enzymatic researchAQUEOUS BIPHASIC SYSTEMSBOVINE SERUM-ALBUMINALPHA-CHYMOTRYPSINDIRECT ELECTROCHEMISTRYCARBON NANOTUBES2-PHASE SYSTEMSGOODS BUFFERSPH BUFFERSSOLVENTSPROTEINIn the present work, we have designed five new biocompatible, self-buffering ionic liquids (ILs) in which the cationic part is derived from conventional tetra-butylphosphonium (TBP) and the anionic part is derived from common biological buffers TAPS, MOPS, EPPS, CAPS, and BICINE. The new ionic liquid based biocompatible media ([TBP][TAPS], [TBP][MOPS], [TBP][EPPS], [TBP][CAPS], and [TBP][BICINE]) were found to be suitable for overcoming most of the problems associated with enzymatic research including optimum pH range, biocompatibility, and extraction. In comparison with the conventional ionic liquid based biological media, these new media do not involve an external buffering compound and maintain the optimum pH by their self-buffering capability. The buffering nature of these new ILs was confirmed by measuring their pH profiles and protonation constants in aqueous solutions at different temperatures. The biocompatibility of these new ILs was also confirmed by measuring the biological activity of the enzyme alpha-chymotrypsin (alpha-CT) in the aqueous media of these ILs. Moreover, these new ionic liquids can form an aqueous two phase system (ATPS) with a common inorganic salt such as sodium sulfate. The excellent extraction efficiency (100%) of these ionic liquid-based ATPS was observed for the extraction of enzyme alpha-CT, in its active form, in an IL-rich phase via a single stage extraction. Since the selected common biological buffers are biocompatible and nontoxic compounds, the ionic liquids derived from these buffer compounds could be more promising for biological research.ROYAL SOC CHEMISTRY2017-12-07T19:51:49Z2015-01-01T00:00:00Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/20570eng2046-206910.1039/c5ra16317jGupta, Bhupender S.Taha, MohamedLee, Ming-Jerinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:40:26Zoai:ria.ua.pt:10773/20570Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:55:14.916903Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
title |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
spellingShingle |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research Gupta, Bhupender S. AQUEOUS BIPHASIC SYSTEMS BOVINE SERUM-ALBUMIN ALPHA-CHYMOTRYPSIN DIRECT ELECTROCHEMISTRY CARBON NANOTUBES 2-PHASE SYSTEMS GOODS BUFFERS PH BUFFERS SOLVENTS PROTEIN |
title_short |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
title_full |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
title_fullStr |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
title_full_unstemmed |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
title_sort |
Self-buffering and biocompatible ionic liquid based biological media for enzymatic research |
author |
Gupta, Bhupender S. |
author_facet |
Gupta, Bhupender S. Taha, Mohamed Lee, Ming-Jer |
author_role |
author |
author2 |
Taha, Mohamed Lee, Ming-Jer |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Gupta, Bhupender S. Taha, Mohamed Lee, Ming-Jer |
dc.subject.por.fl_str_mv |
AQUEOUS BIPHASIC SYSTEMS BOVINE SERUM-ALBUMIN ALPHA-CHYMOTRYPSIN DIRECT ELECTROCHEMISTRY CARBON NANOTUBES 2-PHASE SYSTEMS GOODS BUFFERS PH BUFFERS SOLVENTS PROTEIN |
topic |
AQUEOUS BIPHASIC SYSTEMS BOVINE SERUM-ALBUMIN ALPHA-CHYMOTRYPSIN DIRECT ELECTROCHEMISTRY CARBON NANOTUBES 2-PHASE SYSTEMS GOODS BUFFERS PH BUFFERS SOLVENTS PROTEIN |
description |
In the present work, we have designed five new biocompatible, self-buffering ionic liquids (ILs) in which the cationic part is derived from conventional tetra-butylphosphonium (TBP) and the anionic part is derived from common biological buffers TAPS, MOPS, EPPS, CAPS, and BICINE. The new ionic liquid based biocompatible media ([TBP][TAPS], [TBP][MOPS], [TBP][EPPS], [TBP][CAPS], and [TBP][BICINE]) were found to be suitable for overcoming most of the problems associated with enzymatic research including optimum pH range, biocompatibility, and extraction. In comparison with the conventional ionic liquid based biological media, these new media do not involve an external buffering compound and maintain the optimum pH by their self-buffering capability. The buffering nature of these new ILs was confirmed by measuring their pH profiles and protonation constants in aqueous solutions at different temperatures. The biocompatibility of these new ILs was also confirmed by measuring the biological activity of the enzyme alpha-chymotrypsin (alpha-CT) in the aqueous media of these ILs. Moreover, these new ionic liquids can form an aqueous two phase system (ATPS) with a common inorganic salt such as sodium sulfate. The excellent extraction efficiency (100%) of these ionic liquid-based ATPS was observed for the extraction of enzyme alpha-CT, in its active form, in an IL-rich phase via a single stage extraction. Since the selected common biological buffers are biocompatible and nontoxic compounds, the ionic liquids derived from these buffer compounds could be more promising for biological research. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01T00:00:00Z 2015 2017-12-07T19:51:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/20570 |
url |
http://hdl.handle.net/10773/20570 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2046-2069 10.1039/c5ra16317j |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ROYAL SOC CHEMISTRY |
publisher.none.fl_str_mv |
ROYAL SOC CHEMISTRY |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137605595430912 |