Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes

Detalhes bibliográficos
Autor(a) principal: Lima, M. R. M.
Data de Publicação: 2012
Outros Autores: Ferreres, F., Dias, Alberto Carlos Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/15572
Resumo: Cell suspension cultures of Vitis vinifera cv. Vinhão (Vv) were used to study the putative response of V. vinifera to Phaeomoniella chlamydospora (Pc), a fungus frequently associated with esca and grapevine decline. Cells were elicited with a Pc autoclaved biomass extract and methyl jasmonate (MeJ). Phenolic production was evaluated by HPLC-DAD and HPLC-MS/MS. Phenolic production of Vv cells significantly changes after elicitation. Compared to control, Vv cells elicited by Pc extract increase their stilbene production 20-fold and those elicited by MeJ increase stilbenic production 9-fold. In both cases, there is de novo production of viniferin type compounds. We also analyzed the oxidative burst of Vv cells after elicitation with Pc extract and MeJ, using the probe 2′,7′-dichlorodihydrofluorescein diacetate. Adding Pc extract induces an oxidative burst thatshows a biphasic pattern in Vv cells. Moreover, the induction of 7 defence-related genes expression in Vvcell cultures upon Pc extract elicitation was investigated employing semi-quantitative RT-PCR. Elicitation increases the expression of class 6 and class 10 pathogenesis-related proteins, β-1,3-glucanase, class III chitinase, lipoxygenase, phenylalanine ammonia lyase and stilbene synthase. Therefore, Vv in vitro cell cultures could be an important tool to study esca disease, since they offer a simple, rapid and selective way to evaluate plant/fungus interactions.
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spelling Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genesVitis viniferaEsca diseaseDefence-related genesOxidative burstPhenolic productionCell suspensionsScience & TechnologyCell suspension cultures of Vitis vinifera cv. Vinhão (Vv) were used to study the putative response of V. vinifera to Phaeomoniella chlamydospora (Pc), a fungus frequently associated with esca and grapevine decline. Cells were elicited with a Pc autoclaved biomass extract and methyl jasmonate (MeJ). Phenolic production was evaluated by HPLC-DAD and HPLC-MS/MS. Phenolic production of Vv cells significantly changes after elicitation. Compared to control, Vv cells elicited by Pc extract increase their stilbene production 20-fold and those elicited by MeJ increase stilbenic production 9-fold. In both cases, there is de novo production of viniferin type compounds. We also analyzed the oxidative burst of Vv cells after elicitation with Pc extract and MeJ, using the probe 2′,7′-dichlorodihydrofluorescein diacetate. Adding Pc extract induces an oxidative burst thatshows a biphasic pattern in Vv cells. Moreover, the induction of 7 defence-related genes expression in Vvcell cultures upon Pc extract elicitation was investigated employing semi-quantitative RT-PCR. Elicitation increases the expression of class 6 and class 10 pathogenesis-related proteins, β-1,3-glucanase, class III chitinase, lipoxygenase, phenylalanine ammonia lyase and stilbene synthase. Therefore, Vv in vitro cell cultures could be an important tool to study esca disease, since they offer a simple, rapid and selective way to evaluate plant/fungus interactions.Fundação para a Ciência e a Tecnologia (FCT)SpringerUniversidade do MinhoLima, M. R. M.Ferreres, F.Dias, Alberto Carlos Pires2012-012012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/15572eng0929-187310.1007/s10658-011-9857-4http://www.springerlink.com/content/am133712401l1415/fulltext.pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:49:42Zoai:repositorium.sdum.uminho.pt:1822/15572Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:49:42Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
title Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
spellingShingle Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
Lima, M. R. M.
Vitis vinifera
Esca disease
Defence-related genes
Oxidative burst
Phenolic production
Cell suspensions
Science & Technology
title_short Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
title_full Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
title_fullStr Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
title_full_unstemmed Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
title_sort Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : changes in phenolic production, oxidative state and expression of defence-related genes
author Lima, M. R. M.
author_facet Lima, M. R. M.
Ferreres, F.
Dias, Alberto Carlos Pires
author_role author
author2 Ferreres, F.
Dias, Alberto Carlos Pires
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lima, M. R. M.
Ferreres, F.
Dias, Alberto Carlos Pires
dc.subject.por.fl_str_mv Vitis vinifera
Esca disease
Defence-related genes
Oxidative burst
Phenolic production
Cell suspensions
Science & Technology
topic Vitis vinifera
Esca disease
Defence-related genes
Oxidative burst
Phenolic production
Cell suspensions
Science & Technology
description Cell suspension cultures of Vitis vinifera cv. Vinhão (Vv) were used to study the putative response of V. vinifera to Phaeomoniella chlamydospora (Pc), a fungus frequently associated with esca and grapevine decline. Cells were elicited with a Pc autoclaved biomass extract and methyl jasmonate (MeJ). Phenolic production was evaluated by HPLC-DAD and HPLC-MS/MS. Phenolic production of Vv cells significantly changes after elicitation. Compared to control, Vv cells elicited by Pc extract increase their stilbene production 20-fold and those elicited by MeJ increase stilbenic production 9-fold. In both cases, there is de novo production of viniferin type compounds. We also analyzed the oxidative burst of Vv cells after elicitation with Pc extract and MeJ, using the probe 2′,7′-dichlorodihydrofluorescein diacetate. Adding Pc extract induces an oxidative burst thatshows a biphasic pattern in Vv cells. Moreover, the induction of 7 defence-related genes expression in Vvcell cultures upon Pc extract elicitation was investigated employing semi-quantitative RT-PCR. Elicitation increases the expression of class 6 and class 10 pathogenesis-related proteins, β-1,3-glucanase, class III chitinase, lipoxygenase, phenylalanine ammonia lyase and stilbene synthase. Therefore, Vv in vitro cell cultures could be an important tool to study esca disease, since they offer a simple, rapid and selective way to evaluate plant/fungus interactions.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/15572
url http://hdl.handle.net/1822/15572
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0929-1873
10.1007/s10658-011-9857-4
http://www.springerlink.com/content/am133712401l1415/fulltext.pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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