Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology

Detalhes bibliográficos
Autor(a) principal: Sarropoulou, Elena
Data de Publicação: 2005
Outros Autores: Kotoulas, Georgios, Power, Deborah, Geisler, Robert
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/5413
Resumo: Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology. Physiol Genomics 23: 182–191, 2005. First published July 26, 2005; doi:10.1152/physiolgenomics.00139.2005.—Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.
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spelling Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technologySparus aurataStress responseGene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology. Physiol Genomics 23: 182–191, 2005. First published July 26, 2005; doi:10.1152/physiolgenomics.00139.2005.—Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.We thank Dr. M. Pankratz and lab for providing the microarray spotting facilities and Dr. C. Seiler for support in generating pictures of the developmental stages of sea bream. Sequences reported in this article have been submitted to the National Center for Biotechnology Information (NCBI) EST database under Accession Nos. CB184056–CB184594 and CV133223–CV133736. Microarray expression data have been submitted to ARRAYExpress under Accession Nos. E-MEXP-181 (experiment) and A-MEXP-110 (array) as well as to the NCBI Omnibus under Accession Nos. GSE 2064 and GSE 1887.American Physiological SocietySapientiaSarropoulou, ElenaKotoulas, GeorgiosPower, DeborahGeisler, Robert2014-10-22T15:31:07Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5413engElena Sarropoulou, Georgios Kotoulas, Deborah M. Power, Robert Geisler, "Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology" in Physiol Genomics. 2005 Oct 17;23(2):182-91.1094-8341AUT: DPO00386;http://dx.doi.org/10.1152/physiolgenomics.00139.2005info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:39Zoai:sapientia.ualg.pt:10400.1/5413Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:29.038847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
title Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
spellingShingle Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
Sarropoulou, Elena
Sparus aurata
Stress response
title_short Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
title_full Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
title_fullStr Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
title_full_unstemmed Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
title_sort Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
author Sarropoulou, Elena
author_facet Sarropoulou, Elena
Kotoulas, Georgios
Power, Deborah
Geisler, Robert
author_role author
author2 Kotoulas, Georgios
Power, Deborah
Geisler, Robert
author2_role author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Sarropoulou, Elena
Kotoulas, Georgios
Power, Deborah
Geisler, Robert
dc.subject.por.fl_str_mv Sparus aurata
Stress response
topic Sparus aurata
Stress response
description Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology. Physiol Genomics 23: 182–191, 2005. First published July 26, 2005; doi:10.1152/physiolgenomics.00139.2005.—Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
2014-10-22T15:31:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/5413
url http://hdl.handle.net/10400.1/5413
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Elena Sarropoulou, Georgios Kotoulas, Deborah M. Power, Robert Geisler, "Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology" in Physiol Genomics. 2005 Oct 17;23(2):182-91.
1094-8341
AUT: DPO00386;
http://dx.doi.org/10.1152/physiolgenomics.00139.2005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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