Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans

Detalhes bibliográficos
Autor(a) principal: Lobo, Jorge
Data de Publicação: 2013
Outros Autores: Costa, Pedro M., Teixeira, Marcos A. L., Ferreira, Maria S. G., Costa, Maria Helena Ferrão Ribeiro da, Costa, Filipe Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/31568
Resumo: This work was supported by FEDER through POFC-COMPETE and by national funds from "Fundacao para a Ciencia e a Tecnologia (FCT)" in the scope of the grants FCOMP-01-0124-FEDER-015429 and PEst-C/BIA/UI4050/2011. Jorge Lobo is supported by a FCT PhD grant (SFRH/BD/69750/2010). Pedro M Costa is supported by a FCT post-doc grant (SFRH/BPD/72564/2010). Filipe O Costa benefitted from the Marie Curie European Reintegration Grant PERG02-GA-2007-224890, provided by the European Commission. The authors would also like to thank Carolina Madeira and Monica Landi for the initial molecular experiments, Luisa Borges and Ronaldo Sousa for the support on collections, Catia Bartilotti (IPMA), Nuno Gomes (CBMA) for the offer of crustacean specimens and Artur Oliveira (CBMA) for the alga specimen.
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spelling Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoansDNA barcodingMarine metazoansTaxonomyUniversal primersSDG 14 - Life Below WaterThis work was supported by FEDER through POFC-COMPETE and by national funds from "Fundacao para a Ciencia e a Tecnologia (FCT)" in the scope of the grants FCOMP-01-0124-FEDER-015429 and PEst-C/BIA/UI4050/2011. Jorge Lobo is supported by a FCT PhD grant (SFRH/BD/69750/2010). Pedro M Costa is supported by a FCT post-doc grant (SFRH/BPD/72564/2010). Filipe O Costa benefitted from the Marie Curie European Reintegration Grant PERG02-GA-2007-224890, provided by the European Commission. The authors would also like to thank Carolina Madeira and Monica Landi for the initial molecular experiments, Luisa Borges and Ronaldo Sousa for the support on collections, Catia Bartilotti (IPMA), Nuno Gomes (CBMA) for the offer of crustacean specimens and Artur Oliveira (CBMA) for the alga specimen.BACKGROUND: Building reference libraries of DNA barcodes is relatively straightforward when specifically designed primers are available to amplify the COI-5P region from a relatively narrow taxonomic group (e.g. single class or single order). DNA barcoding marine communities have been comparatively harder to accomplish due to the broad taxonomic diversity and lack of consistently efficient primers. Although some of the so-called "universal" primers have been relatively successful, they still fail to amplify COI-5P of many marine animal groups, while displaying random success even among species within each group. Here we propose a new pair of primers designed to enhance amplification of the COI-5P region in a wide range of marine organisms. RESULTS: Amplification tests conducted on a wide range of marine animal taxa, rendered possible the first--time sequencing of DNA barcodes from eight separated phyla (Annelida, Arthropoda, Chordata, Cnidaria, Echinodermata, Mollusca, Nemertea and Platyhelminthes), comprising a total of 14 classes, 28 orders, 57 families, 68 genus and 76 species. CONCLUSIONS: These primers demonstrated to be highly cost-effective, which is of key importance for DNA barcoding procedures, such as for building comprehensive DNA barcode libraries of marine communities, where the processing of a large numbers of specimens from a wide variety of marine taxa is compulsory.DCEA - Departamento de Ciências e Engenharia do AmbienteRUNLobo, JorgeCosta, Pedro M.Teixeira, Marcos A. L.Ferreira, Maria S. G.Costa, Maria Helena Ferrão Ribeiro daCosta, Filipe Oliveira2018-03-02T23:00:30Z2013-09-102013-09-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/31568eng1472-6785PURE: 258950https://doi.org/10.1186/1472-6785-13-34info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:17:31Zoai:run.unl.pt:10362/31568Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:29:42.272694Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
title Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
spellingShingle Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
Lobo, Jorge
DNA barcoding
Marine metazoans
Taxonomy
Universal primers
SDG 14 - Life Below Water
title_short Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
title_full Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
title_fullStr Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
title_full_unstemmed Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
title_sort Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
author Lobo, Jorge
author_facet Lobo, Jorge
Costa, Pedro M.
Teixeira, Marcos A. L.
Ferreira, Maria S. G.
Costa, Maria Helena Ferrão Ribeiro da
Costa, Filipe Oliveira
author_role author
author2 Costa, Pedro M.
Teixeira, Marcos A. L.
Ferreira, Maria S. G.
Costa, Maria Helena Ferrão Ribeiro da
Costa, Filipe Oliveira
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DCEA - Departamento de Ciências e Engenharia do Ambiente
RUN
dc.contributor.author.fl_str_mv Lobo, Jorge
Costa, Pedro M.
Teixeira, Marcos A. L.
Ferreira, Maria S. G.
Costa, Maria Helena Ferrão Ribeiro da
Costa, Filipe Oliveira
dc.subject.por.fl_str_mv DNA barcoding
Marine metazoans
Taxonomy
Universal primers
SDG 14 - Life Below Water
topic DNA barcoding
Marine metazoans
Taxonomy
Universal primers
SDG 14 - Life Below Water
description This work was supported by FEDER through POFC-COMPETE and by national funds from "Fundacao para a Ciencia e a Tecnologia (FCT)" in the scope of the grants FCOMP-01-0124-FEDER-015429 and PEst-C/BIA/UI4050/2011. Jorge Lobo is supported by a FCT PhD grant (SFRH/BD/69750/2010). Pedro M Costa is supported by a FCT post-doc grant (SFRH/BPD/72564/2010). Filipe O Costa benefitted from the Marie Curie European Reintegration Grant PERG02-GA-2007-224890, provided by the European Commission. The authors would also like to thank Carolina Madeira and Monica Landi for the initial molecular experiments, Luisa Borges and Ronaldo Sousa for the support on collections, Catia Bartilotti (IPMA), Nuno Gomes (CBMA) for the offer of crustacean specimens and Artur Oliveira (CBMA) for the alga specimen.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-10
2013-09-10T00:00:00Z
2018-03-02T23:00:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/31568
url http://hdl.handle.net/10362/31568
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1472-6785
PURE: 258950
https://doi.org/10.1186/1472-6785-13-34
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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