Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Portela, Raquel
Data de Publicação: 2022
Outros Autores: Faria, Nuno A., Miragaia, Maria, Mwangi, Michael, de Lencastre, Hermínia, Tomasz, Alexander, Sobral, Rita G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146332
Resumo: This work was financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. This work was also supported by FCT through Projects LISBOA-01-0145-FEDER007660 (Microbiologia Molecular, Estrutural e Celular); and by ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI—“Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa2020” and by national funds through FCT.
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spelling Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureusantimicrobial resistancecell wallDNA recombinationmethicillin resistant Staphylococcus aureuRho termination of transcription factorPhysiologyEcologyImmunology and Microbiology(all)GeneticsMicrobiology (medical)Cell BiologyInfectious DiseasesThis work was financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. This work was also supported by FCT through Projects LISBOA-01-0145-FEDER007660 (Microbiologia Molecular, Estrutural e Celular); and by ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI—“Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa2020” and by national funds through FCT.In a study of antibiotic resistance in Staphylococcus aureus, specific cell wall mutants were previously generated for the peptidoglycan biosynthesis gene murF, by the insertion of an integrative plasmid. A collection of 30 independent mutants was obtained, and all harbored a variable number of copies of the inserted plasmid, arranged in tandem in the chromosome. Of the 30 mutants, only 3, F9, F20 and F26, with a lower number of plasmid copies, showed an altered peptidoglycan structure, lower resistance to β-lactams and a different loss-of-function mutation in rho gene, that encodes a transcription termination factor. The rho mutations were found to correlate with the level of oxacillin resistance, since genetic complementation with rho gene reestablished the resistance and cell wall parental profile in F9, F20 and F26 strains. Furthermore, complementation with rho resulted in the amplification of the number of plasmid tandem repeats, suggesting that Rho enabled events of recombination that favored a rearrangement in the chromosome in the region of the impaired murF gene. Although the full mechanism of reversion of the cell wall damage was not fully elucidated, we showed that Rho is involved in the recombination process that mediates the tandem amplification of exogeneous DNA fragments inserted into the chromosomeUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaMolecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNPortela, RaquelFaria, Nuno A.Miragaia, MariaMwangi, Michaelde Lencastre, HermíniaTomasz, AlexanderSobral, Rita G.2022-12-16T22:14:38Z2022-09-122022-09-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/146332eng2165-0497PURE: 42763543https://doi.org/10.1128/spectrum.02483-21info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:25Zoai:run.unl.pt:10362/146332Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:35.820664Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
title Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
spellingShingle Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
Portela, Raquel
antimicrobial resistance
cell wall
DNA recombination
methicillin resistant Staphylococcus aureu
Rho termination of transcription factor
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
title_short Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
title_full Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
title_fullStr Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
title_full_unstemmed Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
title_sort Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus
author Portela, Raquel
author_facet Portela, Raquel
Faria, Nuno A.
Miragaia, Maria
Mwangi, Michael
de Lencastre, Hermínia
Tomasz, Alexander
Sobral, Rita G.
author_role author
author2 Faria, Nuno A.
Miragaia, Maria
Mwangi, Michael
de Lencastre, Hermínia
Tomasz, Alexander
Sobral, Rita G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
Molecular, Structural and Cellular Microbiology (MOSTMICRO)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Portela, Raquel
Faria, Nuno A.
Miragaia, Maria
Mwangi, Michael
de Lencastre, Hermínia
Tomasz, Alexander
Sobral, Rita G.
dc.subject.por.fl_str_mv antimicrobial resistance
cell wall
DNA recombination
methicillin resistant Staphylococcus aureu
Rho termination of transcription factor
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
topic antimicrobial resistance
cell wall
DNA recombination
methicillin resistant Staphylococcus aureu
Rho termination of transcription factor
Physiology
Ecology
Immunology and Microbiology(all)
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases
description This work was financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. This work was also supported by FCT through Projects LISBOA-01-0145-FEDER007660 (Microbiologia Molecular, Estrutural e Celular); and by ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI—“Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa2020” and by national funds through FCT.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-16T22:14:38Z
2022-09-12
2022-09-12T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146332
url http://hdl.handle.net/10362/146332
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2165-0497
PURE: 42763543
https://doi.org/10.1128/spectrum.02483-21
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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