Non-conventional yeasts as hosts for heterologous protein production.
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/10929 |
Resumo: | Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae. |
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Non-conventional yeasts as hosts for heterologous protein production.Kluyveromyces lactisYarrowia lipolyticaPichia pastorisHansenula polymorphaHeterologous proteinYeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae.Spanish Society for MicrobiologySpringer-Verlag IbéricaBiblioteca Digital do IPBDominguez, ÁngelFermiñán, EncarnaciónSánchez, ManuelGonzález, Francisco M.Pérez-Campo, Flor MariaGarcía, SusanaHerrero, Ana B.San Vicente, AvelinoCabello, JuanPrado, MarcianoIglesias, Francisco J.Choupina, AltinoBurguillo, Francisco J.Fernández-Lago, LuisLópez, Maria Carmen2014-10-21T10:01:39Z19981998-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/10929engDomínguez, A.; Fermiñán, E.; Sánchez, M.; González, Francisco; Pérez-Campo, F. M.; García, S.; Herrero, A.B.; San Vicente, A.; Cabello, J.; Prado, M.; Iglesias, F.J.; Choupina, Altino; Burguillo, F.J.; Fernández-Lago, L.; López, M.C. (1988). Non-conventional yeasts as hosts for heterologous protein production. International Microbiology. ISSN 1139-6709. 1, p. 131–1421139-6709info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:16:02Zoai:bibliotecadigital.ipb.pt:10198/10929Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:58:22.705410Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Non-conventional yeasts as hosts for heterologous protein production. |
title |
Non-conventional yeasts as hosts for heterologous protein production. |
spellingShingle |
Non-conventional yeasts as hosts for heterologous protein production. Dominguez, Ángel Kluyveromyces lactis Yarrowia lipolytica Pichia pastoris Hansenula polymorpha Heterologous protein |
title_short |
Non-conventional yeasts as hosts for heterologous protein production. |
title_full |
Non-conventional yeasts as hosts for heterologous protein production. |
title_fullStr |
Non-conventional yeasts as hosts for heterologous protein production. |
title_full_unstemmed |
Non-conventional yeasts as hosts for heterologous protein production. |
title_sort |
Non-conventional yeasts as hosts for heterologous protein production. |
author |
Dominguez, Ángel |
author_facet |
Dominguez, Ángel Fermiñán, Encarnación Sánchez, Manuel González, Francisco M. Pérez-Campo, Flor Maria García, Susana Herrero, Ana B. San Vicente, Avelino Cabello, Juan Prado, Marciano Iglesias, Francisco J. Choupina, Altino Burguillo, Francisco J. Fernández-Lago, Luis López, Maria Carmen |
author_role |
author |
author2 |
Fermiñán, Encarnación Sánchez, Manuel González, Francisco M. Pérez-Campo, Flor Maria García, Susana Herrero, Ana B. San Vicente, Avelino Cabello, Juan Prado, Marciano Iglesias, Francisco J. Choupina, Altino Burguillo, Francisco J. Fernández-Lago, Luis López, Maria Carmen |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Dominguez, Ángel Fermiñán, Encarnación Sánchez, Manuel González, Francisco M. Pérez-Campo, Flor Maria García, Susana Herrero, Ana B. San Vicente, Avelino Cabello, Juan Prado, Marciano Iglesias, Francisco J. Choupina, Altino Burguillo, Francisco J. Fernández-Lago, Luis López, Maria Carmen |
dc.subject.por.fl_str_mv |
Kluyveromyces lactis Yarrowia lipolytica Pichia pastoris Hansenula polymorpha Heterologous protein |
topic |
Kluyveromyces lactis Yarrowia lipolytica Pichia pastoris Hansenula polymorpha Heterologous protein |
description |
Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 1998-01-01T00:00:00Z 2014-10-21T10:01:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/10929 |
url |
http://hdl.handle.net/10198/10929 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Domínguez, A.; Fermiñán, E.; Sánchez, M.; González, Francisco; Pérez-Campo, F. M.; García, S.; Herrero, A.B.; San Vicente, A.; Cabello, J.; Prado, M.; Iglesias, F.J.; Choupina, Altino; Burguillo, F.J.; Fernández-Lago, L.; López, M.C. (1988). Non-conventional yeasts as hosts for heterologous protein production. International Microbiology. ISSN 1139-6709. 1, p. 131–142 1139-6709 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer-Verlag Ibérica |
publisher.none.fl_str_mv |
Springer-Verlag Ibérica |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135202904113152 |