Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/19269 |
Resumo: | Native tissues present complex architectures at the micro- and nanoscale that dictate their biological function. Several microfabrication techniques have been employed for engineering polymeric surfaces that could replicate in vitro these micro- and nanofeatures. In this study, biomimetic surfaces of poly(butylene succinate) (PBS) were engineered by a micromolding technique. After the optimization of the system parameters, 20 surfaces with different combinations of groove and ridge sizes were developed and characterized by scanning electron microscopy (SEM). The influence of the engineered microfeatures over the viability and attachment of human adipose derived adult stem cells (hASCs) was evaluated. hASCs cultured onto the engineered surfaces were demonstrated to remain viable for all tested patterns. SEM and immunostaining showed adequate attachment and spreading of the stem cells for all the patterned groove/ridge combinations. This study indicated that it is possible to engineer micropatterned surfaces of PBS and that the developed structures could have great potential for tissue engineering where cell alignment is an essential requisite. |
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Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineeringPoly(butylene succinate)MicromoldingMicrofeaturesHuman adipose stem cellsScience & TechnologyNative tissues present complex architectures at the micro- and nanoscale that dictate their biological function. Several microfabrication techniques have been employed for engineering polymeric surfaces that could replicate in vitro these micro- and nanofeatures. In this study, biomimetic surfaces of poly(butylene succinate) (PBS) were engineered by a micromolding technique. After the optimization of the system parameters, 20 surfaces with different combinations of groove and ridge sizes were developed and characterized by scanning electron microscopy (SEM). The influence of the engineered microfeatures over the viability and attachment of human adipose derived adult stem cells (hASCs) was evaluated. hASCs cultured onto the engineered surfaces were demonstrated to remain viable for all tested patterns. SEM and immunostaining showed adequate attachment and spreading of the stem cells for all the patterned groove/ridge combinations. This study indicated that it is possible to engineer micropatterned surfaces of PBS and that the developed structures could have great potential for tissue engineering where cell alignment is an essential requisite.Fundação para a Ciência e a Tecnologia (FCT)MIT-Portugal ProgramNoE EXPERTISSUES (NMP3-CT-2004-500283)ElsevierUniversidade do MinhoCoutinho, Daniela F.Gomes, Manuela E.Neves, N. M.Reis, R. L.2012-042012-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/19269eng1742-706110.1016/j.actbio.2011.12.03522266028info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:50Zoai:repositorium.sdum.uminho.pt:1822/19269Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:10.284136Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
title |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
spellingShingle |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering Coutinho, Daniela F. Poly(butylene succinate) Micromolding Microfeatures Human adipose stem cells Science & Technology |
title_short |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
title_full |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
title_fullStr |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
title_full_unstemmed |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
title_sort |
Development of micro-patterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering |
author |
Coutinho, Daniela F. |
author_facet |
Coutinho, Daniela F. Gomes, Manuela E. Neves, N. M. Reis, R. L. |
author_role |
author |
author2 |
Gomes, Manuela E. Neves, N. M. Reis, R. L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Coutinho, Daniela F. Gomes, Manuela E. Neves, N. M. Reis, R. L. |
dc.subject.por.fl_str_mv |
Poly(butylene succinate) Micromolding Microfeatures Human adipose stem cells Science & Technology |
topic |
Poly(butylene succinate) Micromolding Microfeatures Human adipose stem cells Science & Technology |
description |
Native tissues present complex architectures at the micro- and nanoscale that dictate their biological function. Several microfabrication techniques have been employed for engineering polymeric surfaces that could replicate in vitro these micro- and nanofeatures. In this study, biomimetic surfaces of poly(butylene succinate) (PBS) were engineered by a micromolding technique. After the optimization of the system parameters, 20 surfaces with different combinations of groove and ridge sizes were developed and characterized by scanning electron microscopy (SEM). The influence of the engineered microfeatures over the viability and attachment of human adipose derived adult stem cells (hASCs) was evaluated. hASCs cultured onto the engineered surfaces were demonstrated to remain viable for all tested patterns. SEM and immunostaining showed adequate attachment and spreading of the stem cells for all the patterned groove/ridge combinations. This study indicated that it is possible to engineer micropatterned surfaces of PBS and that the developed structures could have great potential for tissue engineering where cell alignment is an essential requisite. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04 2012-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/19269 |
url |
http://hdl.handle.net/1822/19269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1742-7061 10.1016/j.actbio.2011.12.035 22266028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133044553023488 |