Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels

Detalhes bibliográficos
Autor(a) principal: Lima, Cristóvão F.
Data de Publicação: 2006
Outros Autores: Ferreira, Manuel Fernandes, Wilson, Cristina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/5374
Resumo: Prova tipográfica (In Press)
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spelling Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levelsPhenolic compoundsLiverOxidative stressHepG2 cellsTert-butyl hydroperoxideAntioxidantsScience & TechnologyProva tipográfica (In Press)In the present work, the potential hepatoprotective effects of five phenolic compounds were evaluated against oxidative damages induced by tert-butyl hydroperoxide (t-BHP) in HepG2 cells in order to relate in vitro antioxidant activity with cytoprotective effects. t-BHP induced considerable cell damages to HepG2 cells as shown by significant LDH leakage, increased lipid peroxidation, DNA damage as well as decreased reduced glutathione (GSH) levels. All tested phenolic compounds significantly decreased cell death induced by t-BHP (when in co-incubation). If the effects of quercetin are given the reference value 1, the compounds rank in the following order according to inhibition of cell death: luteolin (4.0) > quercetin (1.0) > rosmarinic acid (0.34) > luteolin-7-glucoside (0.30) > caffeic acid (0.21). The results underscore the importance of the compound’s lipophilicity in addition to its antioxidant potential for its biological activity. All tested phenolic compounds were found to significantly decrease lipid peroxidation and prevent GSH depletion induced by t-BHP, but only luteolin and quercetin significantly decreased DNA damage. Therefore, the lipophilicity of the natural antioxidants tested appeared to be of even higher importance for DNA protection than for cell survival. The protective potential against cell death was probably achieved mainly by preventing intracellular GSH depletion. The phenolic compounds studied here showed protective potential against oxidative damages induced in HepG2 cells that could be beneficial against liver diseases where it is known that oxidative stress plays a crucial role.Fundação para a Ciência e Tecnologia -SFRH/BD/6942/2001; POCTI/AGR/62040/2004.Pergamon-Elsevier Science LtdUniversidade do MinhoLima, Cristóvão F.Ferreira, Manuel FernandesWilson, Cristina Pereira20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5374eng"Life Sciences". (2006).0024-320510.1016/j.lfs.2006.06.04216857214http://www.sciencedirect.com/science?_ ob=ArticleListURL&_method=list&_ ArticleListID=449971903&_sort=d&view= c&_acct=C000057396&_version=1&_ urlVersion=0&_userid=2459786&md5= 71e9f650450955ed50b43a73ae4ae717info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:25Zoai:repositorium.sdum.uminho.pt:1822/5374Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:25.317884Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
title Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
spellingShingle Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
Lima, Cristóvão F.
Phenolic compounds
Liver
Oxidative stress
HepG2 cells
Tert-butyl hydroperoxide
Antioxidants
Science & Technology
title_short Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
title_full Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
title_fullStr Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
title_full_unstemmed Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
title_sort Phenolic compounds protect HepG2 cells from oxidative damage : relevance of glutathione levels
author Lima, Cristóvão F.
author_facet Lima, Cristóvão F.
Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
author_role author
author2 Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lima, Cristóvão F.
Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
dc.subject.por.fl_str_mv Phenolic compounds
Liver
Oxidative stress
HepG2 cells
Tert-butyl hydroperoxide
Antioxidants
Science & Technology
topic Phenolic compounds
Liver
Oxidative stress
HepG2 cells
Tert-butyl hydroperoxide
Antioxidants
Science & Technology
description Prova tipográfica (In Press)
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/5374
url http://hdl.handle.net/1822/5374
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Life Sciences". (2006).
0024-3205
10.1016/j.lfs.2006.06.042
16857214
http://www.sciencedirect.com/science?_ ob=ArticleListURL&_method=list&_ ArticleListID=449971903&_sort=d&view= c&_acct=C000057396&_version=1&_ urlVersion=0&_userid=2459786&md5= 71e9f650450955ed50b43a73ae4ae717
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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