Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability

Detalhes bibliográficos
Autor(a) principal: Carrapita, Jorge
Data de Publicação: 2016
Outros Autores: Abrantes, Ana Margarida, Campelos, Sofia, Gonçalves, Ana Cristina, Cardoso, Dulce, Ribeiro, Ana Bela Sarmento, Rocha, Clara, Santos, Jorge Nunes, Botelho, Maria Filomena, Tralhão, José Guilherme, Farges, Olivier, Barbosa, Jorge Maciel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/108917
https://doi.org/10.1038/srep34731
Resumo: It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.
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spelling Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viabilityAnimalsApoptosisCell SurvivalHepatectomyHepatocytesLigationLiverLiver Function TestsLiver RegenerationMaleMembrane Potential, MitochondrialNecrosisOxidative StressPrimary Cell CultureRatsRats, WistarSpleenSplenic ArterySuperoxidesSurvival AnalysisIt was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.Springer Nature2016-10-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108917http://hdl.handle.net/10316/108917https://doi.org/10.1038/srep34731eng2045-2322Carrapita, JorgeAbrantes, Ana MargaridaCampelos, SofiaGonçalves, Ana CristinaCardoso, DulceRibeiro, Ana Bela SarmentoRocha, ClaraSantos, Jorge NunesBotelho, Maria FilomenaTralhão, José GuilhermeFarges, OlivierBarbosa, Jorge Macielinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-25T09:18:18Zoai:estudogeral.uc.pt:10316/108917Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:09.281418Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
title Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
spellingShingle Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
Carrapita, Jorge
Animals
Apoptosis
Cell Survival
Hepatectomy
Hepatocytes
Ligation
Liver
Liver Function Tests
Liver Regeneration
Male
Membrane Potential, Mitochondrial
Necrosis
Oxidative Stress
Primary Cell Culture
Rats
Rats, Wistar
Spleen
Splenic Artery
Superoxides
Survival Analysis
title_short Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
title_full Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
title_fullStr Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
title_full_unstemmed Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
title_sort Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability
author Carrapita, Jorge
author_facet Carrapita, Jorge
Abrantes, Ana Margarida
Campelos, Sofia
Gonçalves, Ana Cristina
Cardoso, Dulce
Ribeiro, Ana Bela Sarmento
Rocha, Clara
Santos, Jorge Nunes
Botelho, Maria Filomena
Tralhão, José Guilherme
Farges, Olivier
Barbosa, Jorge Maciel
author_role author
author2 Abrantes, Ana Margarida
Campelos, Sofia
Gonçalves, Ana Cristina
Cardoso, Dulce
Ribeiro, Ana Bela Sarmento
Rocha, Clara
Santos, Jorge Nunes
Botelho, Maria Filomena
Tralhão, José Guilherme
Farges, Olivier
Barbosa, Jorge Maciel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carrapita, Jorge
Abrantes, Ana Margarida
Campelos, Sofia
Gonçalves, Ana Cristina
Cardoso, Dulce
Ribeiro, Ana Bela Sarmento
Rocha, Clara
Santos, Jorge Nunes
Botelho, Maria Filomena
Tralhão, José Guilherme
Farges, Olivier
Barbosa, Jorge Maciel
dc.subject.por.fl_str_mv Animals
Apoptosis
Cell Survival
Hepatectomy
Hepatocytes
Ligation
Liver
Liver Function Tests
Liver Regeneration
Male
Membrane Potential, Mitochondrial
Necrosis
Oxidative Stress
Primary Cell Culture
Rats
Rats, Wistar
Spleen
Splenic Artery
Superoxides
Survival Analysis
topic Animals
Apoptosis
Cell Survival
Hepatectomy
Hepatocytes
Ligation
Liver
Liver Function Tests
Liver Regeneration
Male
Membrane Potential, Mitochondrial
Necrosis
Oxidative Stress
Primary Cell Culture
Rats
Rats, Wistar
Spleen
Splenic Artery
Superoxides
Survival Analysis
description It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/108917
http://hdl.handle.net/10316/108917
https://doi.org/10.1038/srep34731
url http://hdl.handle.net/10316/108917
https://doi.org/10.1038/srep34731
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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