Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples

Detalhes bibliográficos
Autor(a) principal: Sabino, Raquel
Data de Publicação: 2020
Outros Autores: Simões, Helena, Veríssimo, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7372
Resumo: Brief Report
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spelling Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue SamplesAspergillusInvasive Fungal InfectionsReal-time PCRMolecular DiagnosisAzole-resistanceInfecções Sistémicas e ZoonosesBrief ReportDiagnosis of invasive fungal infections is complex, and the lack of standardization of molecular methods is still a challenge. Several methods are available for the diagnosis of invasive aspergillosis, but their effectiveness will depend on the studied population, the patients' comorbidities, and the use of mold active prophylaxis, among others. The ability to determine the identity of the infecting Aspergillus species, and to detect mutations conferring specific resistance patterns directly from DNA extracted from the biological product, is an advantage of nucleic acid testing compared with antigen-based assays. In this study, we to present laboratory cases where the diagnosis of aspergillosis was performed using a real-time multiplex PCR for the detection of Aspergillus DNA in tissue samples, showing its usefulness as one more tool in the diagnosis of aspergillosis in tissue samples. Aspergillus real-time multiplex PCR was also used to detect azole-resistance in some cases. In the majority of the PCR positive cases, cultures remained negative after 60 days. The PCR assay directed to Aspergillus gave positive signals for Aspergillus fumigatus sensu stricto. Results were confirmed by panfungal PCR, followed by sequencing, revealing 100% homology with Aspergillus fumigatus sensu stricto. Mutations conferring azole resistance were not detected.MDPIRepositório Científico do Instituto Nacional de SaúdeSabino, RaquelSimões, HelenaVeríssimo, Cristina2021-03-09T15:42:04Z2020-01-102020-01-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7372engJ Fungi (Basel). 2020 Jan 10;6(1):11. doi: 10.3390/jof6010011.2309-608X10.3390/jof6010011info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:58Zoai:repositorio.insa.pt:10400.18/7372Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:01.590528Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
title Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
spellingShingle Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
Sabino, Raquel
Aspergillus
Invasive Fungal Infections
Real-time PCR
Molecular Diagnosis
Azole-resistance
Infecções Sistémicas e Zoonoses
title_short Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
title_full Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
title_fullStr Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
title_full_unstemmed Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
title_sort Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples
author Sabino, Raquel
author_facet Sabino, Raquel
Simões, Helena
Veríssimo, Cristina
author_role author
author2 Simões, Helena
Veríssimo, Cristina
author2_role author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Sabino, Raquel
Simões, Helena
Veríssimo, Cristina
dc.subject.por.fl_str_mv Aspergillus
Invasive Fungal Infections
Real-time PCR
Molecular Diagnosis
Azole-resistance
Infecções Sistémicas e Zoonoses
topic Aspergillus
Invasive Fungal Infections
Real-time PCR
Molecular Diagnosis
Azole-resistance
Infecções Sistémicas e Zoonoses
description Brief Report
publishDate 2020
dc.date.none.fl_str_mv 2020-01-10
2020-01-10T00:00:00Z
2021-03-09T15:42:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7372
url http://hdl.handle.net/10400.18/7372
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Fungi (Basel). 2020 Jan 10;6(1):11. doi: 10.3390/jof6010011.
2309-608X
10.3390/jof6010011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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