Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/3764 |
Resumo: | Free PMC Article: www.ncbi.nlm.nih.gov/pmc/articles/pmid/26497645/ |
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Co-circulation of a novel phlebovirus and Massilia virus in sandflies, PortugalInfecções Sistémicas e ZoonosesPhlebovirusAlcube VirusSalehabad complexComplete GenomePhylogenyPortugalFree PMC Article: www.ncbi.nlm.nih.gov/pmc/articles/pmid/26497645/Background: In Portugal, entomological surveys to detect phleboviruses in their natural vectors have not been performed so far. Thus, the aims of the present study were to detect, isolate and characterize phleboviruses in sandfly populations of Portugal. Findings: From May to October 2007–2008, 896 female sandflies were trapped in Arrábida region, located on the southwest coast of Portugal. Phlebovirus RNA was detected by using a pan-phlebovirus RT-PCR in 4 out of 34 Phlebotomus perniciosus pools. Direct sequencing of the amplicons showed that 2 samples exhibited 72 % nucleotide identity with Arbia virus, and two showed 96 % nucleotide identity with Massilia virus. The Arbia-like virus (named Alcube virus) was isolated in cell culture and complete genomic sequences of one Alcube and two Massila viruses were determined using next-generation sequencing technology. Phylogenetic analysis demonstrated that Alcube virus clustered with members of the Salehabad virus species complex. Within this clade, Alcube virus forms a monophyletic lineage with the Arbia, Salehabad and Adana viruses sharing a common ancestor. Arbia virus has been identified as the most closely related virus with 20-28 % nucleotide and 10-27 % amino acid divergences depending on the analysed segment. Conclusions: We have provided genetic evidence for the circulation of a novel phlebovirus species named Alcube virus in Ph. perniciosus and co-circulation of Massilia virus, in Arrábida region, southwest of Portugal. Further epidemiological investigations and surveillance for sandfly-borne phleboviruses in Portugal are needed to elucidate their medical importance.This work was partially funded by the FCT project “New arboviruses isolated in Portugal. Risk assessment and public health application" (PTDC/SAU-SAP/119199/2010).BioMed CentralRepositório Científico do Instituto Nacional de SaúdeAmaro, FátimaZé-Zé, LíbiaAlves, M.J.Börstler, J.Clos, J.Lorenzen, S.Becker, S. C.Schmidt-Chanasit, J.Cadar, D.2016-03-30T12:32:19Z2015-10-242015-10-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3764engVirol J. 2015 Oct 24;12:174. doi: 10.1186/s12985-015-0407-01743-422X10.1186/s12985-015-0407-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:56Zoai:repositorio.insa.pt:10400.18/3764Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:32.884328Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| title |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| spellingShingle |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal Amaro, Fátima Infecções Sistémicas e Zoonoses Phlebovirus Alcube Virus Salehabad complex Complete Genome Phylogeny Portugal |
| title_short |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| title_full |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| title_fullStr |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| title_full_unstemmed |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| title_sort |
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal |
| author |
Amaro, Fátima |
| author_facet |
Amaro, Fátima Zé-Zé, Líbia Alves, M.J. Börstler, J. Clos, J. Lorenzen, S. Becker, S. C. Schmidt-Chanasit, J. Cadar, D. |
| author_role |
author |
| author2 |
Zé-Zé, Líbia Alves, M.J. Börstler, J. Clos, J. Lorenzen, S. Becker, S. C. Schmidt-Chanasit, J. Cadar, D. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Amaro, Fátima Zé-Zé, Líbia Alves, M.J. Börstler, J. Clos, J. Lorenzen, S. Becker, S. C. Schmidt-Chanasit, J. Cadar, D. |
| dc.subject.por.fl_str_mv |
Infecções Sistémicas e Zoonoses Phlebovirus Alcube Virus Salehabad complex Complete Genome Phylogeny Portugal |
| topic |
Infecções Sistémicas e Zoonoses Phlebovirus Alcube Virus Salehabad complex Complete Genome Phylogeny Portugal |
| description |
Free PMC Article: www.ncbi.nlm.nih.gov/pmc/articles/pmid/26497645/ |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-10-24 2015-10-24T00:00:00Z 2016-03-30T12:32:19Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3764 |
| url |
http://hdl.handle.net/10400.18/3764 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Virol J. 2015 Oct 24;12:174. doi: 10.1186/s12985-015-0407-0 1743-422X 10.1186/s12985-015-0407-0 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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BioMed Central |
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BioMed Central |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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