Assessment of silver nanoparticles effects: from proteins to species

Detalhes bibliográficos
Autor(a) principal: Fernandes, Marco André Ferreira
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/13345
Resumo: The continuous industrial expansion of engineered silver nanoparticles (AgNPs) in conjugation with their ability to be released from the products to the water will likely enhance their accumulation in environmental and biological matrices. The current level of understanding on the environmental fate and ecotoxicology of AgNPs in the aquatic environment still has some gaps. Thus, the main goal of this thesis is to develop an integrated methodological approach to assess the effects of AgNPs to freshwater organisms at different levels of organization, from sub-individual and sub-cellular endpoints, to individual and extrapolating to the community level effects. . At the sub-individual and sub-cellular levels we studied the biochemical and protein expression responses in Chlamydomonas reinhardtii exposed to AgNPs with different sizes. Concerning the individual levels we studied the effects of AgNPs in the growth of the microalgae Pseudokirchneriella subcapitata and the effects on the survival, growth and reproduction of the crustacean Daphnia magna exposed to AgNPs via the water or the diet. Finally, to extrapolate the effects of AgNPs from the individual to the community level we used the SSD approach.
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spelling Assessment of silver nanoparticles effects: from proteins to speciesBiologiaNanopartículas - PrataPoluição da águaEcotoxicologiaThe continuous industrial expansion of engineered silver nanoparticles (AgNPs) in conjugation with their ability to be released from the products to the water will likely enhance their accumulation in environmental and biological matrices. The current level of understanding on the environmental fate and ecotoxicology of AgNPs in the aquatic environment still has some gaps. Thus, the main goal of this thesis is to develop an integrated methodological approach to assess the effects of AgNPs to freshwater organisms at different levels of organization, from sub-individual and sub-cellular endpoints, to individual and extrapolating to the community level effects. . At the sub-individual and sub-cellular levels we studied the biochemical and protein expression responses in Chlamydomonas reinhardtii exposed to AgNPs with different sizes. Concerning the individual levels we studied the effects of AgNPs in the growth of the microalgae Pseudokirchneriella subcapitata and the effects on the survival, growth and reproduction of the crustacean Daphnia magna exposed to AgNPs via the water or the diet. Finally, to extrapolate the effects of AgNPs from the individual to the community level we used the SSD approach.A contínua expansão industrial de nanopartículas de prata manufacturadas, em conjugação com a sua capacidade para se libertarem de produtos de consumo, directamente para a água, irá certamente aumentar a sua acumulação no ambiente e em matrizes biológicas. O actual nível de conhecimento acerca da ecotoxicologia e destino das AgNPs em ambientes aquáticos, ainda apresenta algumas falhas. Assim, o principal objectivo desta tese, é desenvolver uma abordagem metodológica integrativa, de forma a avaliar os efeitos das AgNPs para os organismos dulçaquícolas pertencentes a diferentes níveis de organização biológica, desde parâmetros sub-individuais e sub-celulares, até individuais, extrapolando os efeitos ao nível da comunidade. Nos níveis sub-individuais e sub-celulares, estudámos as respostas bioquímicas e a expressão proteicas em Chlamydomonas reinhardtii expostas a AgNPs de diferentes tamanhos. Em relação aos níveis individuais, estudámos os efeitos das AgNPs no crescimento da microalga Pseudokirchneriella subcapitata e os efeitos na sobrevivência, crescimento e reprodução do crustáceo Daphnia magna exposto a AgNPs via meio ou através da dieta. De forma a extrapolar os efeitos das AgNPs, desde os indivíduos até ao nível da comunidade utilizamos a abordagem com SSDs.Universidade de Aveiro2018-07-20T14:00:47Z2013-12-18T00:00:00Z2013-12-182015-12-18T17:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/13345TID:201584166engFernandes, Marco André Ferreirainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:24:16Zoai:ria.ua.pt:10773/13345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:49:14.141788Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of silver nanoparticles effects: from proteins to species
title Assessment of silver nanoparticles effects: from proteins to species
spellingShingle Assessment of silver nanoparticles effects: from proteins to species
Fernandes, Marco André Ferreira
Biologia
Nanopartículas - Prata
Poluição da água
Ecotoxicologia
title_short Assessment of silver nanoparticles effects: from proteins to species
title_full Assessment of silver nanoparticles effects: from proteins to species
title_fullStr Assessment of silver nanoparticles effects: from proteins to species
title_full_unstemmed Assessment of silver nanoparticles effects: from proteins to species
title_sort Assessment of silver nanoparticles effects: from proteins to species
author Fernandes, Marco André Ferreira
author_facet Fernandes, Marco André Ferreira
author_role author
dc.contributor.author.fl_str_mv Fernandes, Marco André Ferreira
dc.subject.por.fl_str_mv Biologia
Nanopartículas - Prata
Poluição da água
Ecotoxicologia
topic Biologia
Nanopartículas - Prata
Poluição da água
Ecotoxicologia
description The continuous industrial expansion of engineered silver nanoparticles (AgNPs) in conjugation with their ability to be released from the products to the water will likely enhance their accumulation in environmental and biological matrices. The current level of understanding on the environmental fate and ecotoxicology of AgNPs in the aquatic environment still has some gaps. Thus, the main goal of this thesis is to develop an integrated methodological approach to assess the effects of AgNPs to freshwater organisms at different levels of organization, from sub-individual and sub-cellular endpoints, to individual and extrapolating to the community level effects. . At the sub-individual and sub-cellular levels we studied the biochemical and protein expression responses in Chlamydomonas reinhardtii exposed to AgNPs with different sizes. Concerning the individual levels we studied the effects of AgNPs in the growth of the microalgae Pseudokirchneriella subcapitata and the effects on the survival, growth and reproduction of the crustacean Daphnia magna exposed to AgNPs via the water or the diet. Finally, to extrapolate the effects of AgNPs from the individual to the community level we used the SSD approach.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-18T00:00:00Z
2013-12-18
2015-12-18T17:00:00Z
2018-07-20T14:00:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/13345
TID:201584166
url http://hdl.handle.net/10773/13345
identifier_str_mv TID:201584166
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Aveiro
publisher.none.fl_str_mv Universidade de Aveiro
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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