Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73645 |
Resumo: | Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogensurface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesinligand interaction, supported by present high-throughput omics technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19. |
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Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogensAdhesinReceptorInfectious diseasesDiagnosticsScience & TechnologyInfectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogensurface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesinligand interaction, supported by present high-throughput omics technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19.This research was funded by the European Union’s Horizon 2020 research and innovation program in a project named Viral and Bacterial Adhesin Network Training (ViBrANT) under Marie Skłodowska-Curie Grant Agreement No. 765042. A.G. also acknowledges support from the BBSRC (grant number BB/M021610/1).info:eu-repo/semantics/publishedVersionMDPIUniversidade do Minhovan Belkum, AlexAlmeida, CarinaBardiaux, BenjaminBarrass, Sarah V.Butcher, Sarah J.Çaykara, TugçeChowdhury, SounakDatar, RuchaEastwood, IanGoldman, AdrianGoyal, ManishaHapponen, LottaIzadi-Pruneyre, NadiaJacobsen, TheisJohnson, Pirjo H.Kempf, Volkhard A. J.Kiessling, AndreasBueno, Juan LevaMalik, AnchalMalmström, JohanMeuskens, InaMilner, Paul A.Nilges, MichaelPamme, NicolePeyman, Sally A.Rodrigues, L. R.Rodriguez-Mateos, PabloSande, Maria G.Silva, Carla JoanaStasiak, Aleksandra CecyliaStehle, ThiloThibau, ArnoVaca, Diana J.Linke, Dirk2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73645engvan Belkum, A.; Almeida, C.; Bardiaux, B.; Barrass, S.V.; Butcher, S.J.; Çaykara, T.; Chowdhury, S.; Datar, R.; Eastwood, I.; Goldman, A.; Goyal, M.; Happonen, L.; Izadi-Pruneyre, N.; Jacobsen, T.; Johnson, P.H.; Kempf, V.A.J.; Kiessling, A.; Bueno, J.L.; Malik, A.; Malmström, J.; Meuskens, I.; Milner, P.A.; Nilges, M.; Pamme, N.; Peyman, S.A.; Rodrigues, L.R.; Rodriguez-Mateos, P.; Sande, M.G.; Silva, C.J.; Stasiak, A.C.; Stehle, T.; Thibau, A.; Vaca, D.J.; Linke, D. Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens. Diagnostics 2021, 11, 1259. https://doi.org/10.3390/diagnostics11071259.2075-441810.3390/diagnostics11071259https://www.mdpi.com/2075-4418/11/7/1259info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:12Zoai:repositorium.sdum.uminho.pt:1822/73645Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:46.500121Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
title |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
spellingShingle |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens van Belkum, Alex Adhesin Receptor Infectious diseases Diagnostics Science & Technology |
title_short |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
title_full |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
title_fullStr |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
title_full_unstemmed |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
title_sort |
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens |
author |
van Belkum, Alex |
author_facet |
van Belkum, Alex Almeida, Carina Bardiaux, Benjamin Barrass, Sarah V. Butcher, Sarah J. Çaykara, Tugçe Chowdhury, Sounak Datar, Rucha Eastwood, Ian Goldman, Adrian Goyal, Manisha Happonen, Lotta Izadi-Pruneyre, Nadia Jacobsen, Theis Johnson, Pirjo H. Kempf, Volkhard A. J. Kiessling, Andreas Bueno, Juan Leva Malik, Anchal Malmström, Johan Meuskens, Ina Milner, Paul A. Nilges, Michael Pamme, Nicole Peyman, Sally A. Rodrigues, L. R. Rodriguez-Mateos, Pablo Sande, Maria G. Silva, Carla Joana Stasiak, Aleksandra Cecylia Stehle, Thilo Thibau, Arno Vaca, Diana J. Linke, Dirk |
author_role |
author |
author2 |
Almeida, Carina Bardiaux, Benjamin Barrass, Sarah V. Butcher, Sarah J. Çaykara, Tugçe Chowdhury, Sounak Datar, Rucha Eastwood, Ian Goldman, Adrian Goyal, Manisha Happonen, Lotta Izadi-Pruneyre, Nadia Jacobsen, Theis Johnson, Pirjo H. Kempf, Volkhard A. J. Kiessling, Andreas Bueno, Juan Leva Malik, Anchal Malmström, Johan Meuskens, Ina Milner, Paul A. Nilges, Michael Pamme, Nicole Peyman, Sally A. Rodrigues, L. R. Rodriguez-Mateos, Pablo Sande, Maria G. Silva, Carla Joana Stasiak, Aleksandra Cecylia Stehle, Thilo Thibau, Arno Vaca, Diana J. Linke, Dirk |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
van Belkum, Alex Almeida, Carina Bardiaux, Benjamin Barrass, Sarah V. Butcher, Sarah J. Çaykara, Tugçe Chowdhury, Sounak Datar, Rucha Eastwood, Ian Goldman, Adrian Goyal, Manisha Happonen, Lotta Izadi-Pruneyre, Nadia Jacobsen, Theis Johnson, Pirjo H. Kempf, Volkhard A. J. Kiessling, Andreas Bueno, Juan Leva Malik, Anchal Malmström, Johan Meuskens, Ina Milner, Paul A. Nilges, Michael Pamme, Nicole Peyman, Sally A. Rodrigues, L. R. Rodriguez-Mateos, Pablo Sande, Maria G. Silva, Carla Joana Stasiak, Aleksandra Cecylia Stehle, Thilo Thibau, Arno Vaca, Diana J. Linke, Dirk |
dc.subject.por.fl_str_mv |
Adhesin Receptor Infectious diseases Diagnostics Science & Technology |
topic |
Adhesin Receptor Infectious diseases Diagnostics Science & Technology |
description |
Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogensurface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesinligand interaction, supported by present high-throughput omics technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07 2021-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73645 |
url |
http://hdl.handle.net/1822/73645 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
van Belkum, A.; Almeida, C.; Bardiaux, B.; Barrass, S.V.; Butcher, S.J.; Çaykara, T.; Chowdhury, S.; Datar, R.; Eastwood, I.; Goldman, A.; Goyal, M.; Happonen, L.; Izadi-Pruneyre, N.; Jacobsen, T.; Johnson, P.H.; Kempf, V.A.J.; Kiessling, A.; Bueno, J.L.; Malik, A.; Malmström, J.; Meuskens, I.; Milner, P.A.; Nilges, M.; Pamme, N.; Peyman, S.A.; Rodrigues, L.R.; Rodriguez-Mateos, P.; Sande, M.G.; Silva, C.J.; Stasiak, A.C.; Stehle, T.; Thibau, A.; Vaca, D.J.; Linke, D. Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens. Diagnostics 2021, 11, 1259. https://doi.org/10.3390/diagnostics11071259. 2075-4418 10.3390/diagnostics11071259 https://www.mdpi.com/2075-4418/11/7/1259 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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