Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses

Detalhes bibliográficos
Autor(a) principal: Marquesa, Simone M.
Data de Publicação: 2015
Outros Autores: Santosa, Adriano, Gonçalves, Luís M., Sousa, João Carlos, Bueno, Paulo R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/41535
Resumo: Supplementary data associated with this article can be found, in the online version, at: http://dx.doi.org/10.1016/j.electacta.2015.09.169.
id RCAP_b973016f905f6dc11aedf1e5673b72fb
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/41535
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalysesBiosensorD-dimerElectroanalysisElectrochemical CapacitancePulmonary embolismSelf-assembled monolayerCiências Médicas::Ciências da SaúdeScience & TechnologySupplementary data associated with this article can be found, in the online version, at: http://dx.doi.org/10.1016/j.electacta.2015.09.169.D-dimer, an important biomarker in emergency medicine, was determined by applying a label-free electrochemical capacitive approach with an antibody-based selective biosensor consisting of an electroactive self-assembled monolayer mounted on a commercially available gold electrode. Electrochemical measurements were performed by an impedance-derived approach (based on observing the complex capacitance diagrams from where electrochemical capacitance can be obtained as transducer signal) from which it was possible to obtain low limits of detection of 50 fmol L-1 in buffer (PBS) and 7 pmol L-1 in undiluted human serum (performed without any kind of sample pre-treatment), with good coefficient of determination (r2 = 0.99) in a clinically relevant D-dimer concentration range (from 1 to 1,000 ng mL-1). These electro analytical/chemistry results pave the way to the creation of low-cost and reliable point-of-care electrochemical approach for the quantitative bedside determination of D-dimer in clinical samples, thus speeding up the diagnosis of potentially lethal, nonetheless treatable, clinical conditions like pulmonary embolism.This work was financially supported by the São Paulo Research Foundation (FAPESP, 2012-22820-7) and National Council for Scientific and Technological Development (CNPq) grants. AS acknowledges CNPq for his Ph.D. studentship (141058/2013-7), SMM acknowledges FAPESP for her research grant (2014/030398) and LMG acknowledges the Portuguese Foundation for Science and Technology (FCT) for his post-doctoral fellowship (SFRH/BPD/76544/2011).ElsevierUniversidade do MinhoMarquesa, Simone M.Santosa, AdrianoGonçalves, Luís M.Sousa, João CarlosBueno, Paulo R.2015-10-092015-10-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41535engMarques, S. M., Santos, A., Gonçalves, L. M., Sousa, J. C., & Bueno, P. R. (2015). Sensitive label-free electron chemical capacitive signal transduction for D-dimer electroanalysis. Electrochimica Acta, 182, 946-952. doi: 10.1016/j.electacta.2015.09.1690013-468610.1016/j.electacta.2015.09.169http://www.journals.elsevier.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:19Zoai:repositorium.sdum.uminho.pt:1822/41535Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:05.740708Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
title Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
spellingShingle Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
Marquesa, Simone M.
Biosensor
D-dimer
Electroanalysis
Electrochemical Capacitance
Pulmonary embolism
Self-assembled monolayer
Ciências Médicas::Ciências da Saúde
Science & Technology
title_short Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
title_full Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
title_fullStr Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
title_full_unstemmed Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
title_sort Sensitive label-free electron chemical capacitive transducer signal for D-dimer electroanalyses
author Marquesa, Simone M.
author_facet Marquesa, Simone M.
Santosa, Adriano
Gonçalves, Luís M.
Sousa, João Carlos
Bueno, Paulo R.
author_role author
author2 Santosa, Adriano
Gonçalves, Luís M.
Sousa, João Carlos
Bueno, Paulo R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Marquesa, Simone M.
Santosa, Adriano
Gonçalves, Luís M.
Sousa, João Carlos
Bueno, Paulo R.
dc.subject.por.fl_str_mv Biosensor
D-dimer
Electroanalysis
Electrochemical Capacitance
Pulmonary embolism
Self-assembled monolayer
Ciências Médicas::Ciências da Saúde
Science & Technology
topic Biosensor
D-dimer
Electroanalysis
Electrochemical Capacitance
Pulmonary embolism
Self-assembled monolayer
Ciências Médicas::Ciências da Saúde
Science & Technology
description Supplementary data associated with this article can be found, in the online version, at: http://dx.doi.org/10.1016/j.electacta.2015.09.169.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-09
2015-10-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/41535
url http://hdl.handle.net/1822/41535
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marques, S. M., Santos, A., Gonçalves, L. M., Sousa, J. C., & Bueno, P. R. (2015). Sensitive label-free electron chemical capacitive signal transduction for D-dimer electroanalysis. Electrochimica Acta, 182, 946-952. doi: 10.1016/j.electacta.2015.09.169
0013-4686
10.1016/j.electacta.2015.09.169
http://www.journals.elsevier.com
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132704488292352

Registros relacionados