Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/63341 |
Resumo: | Supplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145. |
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Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infectionsBacteriophagesAlginate-nanohydroxyapatiteBiocompatibilityAntimicrobial activityOsteogenic differentiationScience & TechnologySupplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145.An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control. Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition. Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile. Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology. A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite. Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues. This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.Funding: This work was supported by FEDER – Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, by Portuguese funds through FCT/MCTES in the framework of the project “institute for Research and Innovation in Health Sciences (POCI-01-0145-FEDER-007274), by Project Biotherapies (NORTE-01-0145-FEDER-000012) and by Joana Barrosʼ PhD grant (SFRH/BD/102148/2014). This work was also supported by Portuguese funds through FCT/MCTES (UID/QUI/50006/2019).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoBarros, J.Melo, Luís Daniel RodriguesSilva, R. A.Ferraz, M. P.Azeredo, JoanaPinheiro, V.Colaço, B.Fernandes, M. H.Gomes, P. S.Monteiro, F. J.2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/63341engBarros, J.; Melo, Luís D. R.; Silva, R. A.; Ferraz, M. P.; Azeredo, Joana; Pinheiro, V.; Colaço, B.; Fernandes, M. H.; Gomes, P. S.; Monteiro, F. J., Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine-Nanotechnology Biology and Medicine, 24(102145), 20201549-96341549-964210.1016/j.nano.2019.10214531857183http://www.nanomedjournal.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:58Zoai:repositorium.sdum.uminho.pt:1822/63341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:03.659983Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
title |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
spellingShingle |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections Barros, J. Bacteriophages Alginate-nanohydroxyapatite Biocompatibility Antimicrobial activity Osteogenic differentiation Science & Technology |
title_short |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
title_full |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
title_fullStr |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
title_full_unstemmed |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
title_sort |
Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections |
author |
Barros, J. |
author_facet |
Barros, J. Melo, Luís Daniel Rodrigues Silva, R. A. Ferraz, M. P. Azeredo, Joana Pinheiro, V. Colaço, B. Fernandes, M. H. Gomes, P. S. Monteiro, F. J. |
author_role |
author |
author2 |
Melo, Luís Daniel Rodrigues Silva, R. A. Ferraz, M. P. Azeredo, Joana Pinheiro, V. Colaço, B. Fernandes, M. H. Gomes, P. S. Monteiro, F. J. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Barros, J. Melo, Luís Daniel Rodrigues Silva, R. A. Ferraz, M. P. Azeredo, Joana Pinheiro, V. Colaço, B. Fernandes, M. H. Gomes, P. S. Monteiro, F. J. |
dc.subject.por.fl_str_mv |
Bacteriophages Alginate-nanohydroxyapatite Biocompatibility Antimicrobial activity Osteogenic differentiation Science & Technology |
topic |
Bacteriophages Alginate-nanohydroxyapatite Biocompatibility Antimicrobial activity Osteogenic differentiation Science & Technology |
description |
Supplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2020-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/63341 |
url |
http://hdl.handle.net/1822/63341 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Barros, J.; Melo, Luís D. R.; Silva, R. A.; Ferraz, M. P.; Azeredo, Joana; Pinheiro, V.; Colaço, B.; Fernandes, M. H.; Gomes, P. S.; Monteiro, F. J., Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine-Nanotechnology Biology and Medicine, 24(102145), 2020 1549-9634 1549-9642 10.1016/j.nano.2019.102145 31857183 http://www.nanomedjournal.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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