Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo

Detalhes bibliográficos
Autor(a) principal: Wang, Lingzhi
Data de Publicação: 2023
Outros Autores: Oliveira, Ana Catarina Freitas Salazar, Li, Qiu, Ferreira, Andreia S., Nunes, Cláudia, Coimbra, Manuel A., Reis, R. L., Martins, Albino, Wang, Chunming, Silva, Tiago H., Feng, Yanxian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/85642
Resumo: Fucoidan has been reported to present diverse bioactivities, but each extract has specific features from which a particular biological activity, such as immunomodulation, must be confirmed. In this study a commercially available pharmaceutical-grade fucoidan extracted from Fucus vesiculosus, FE, was characterized and its anti-inflammatory potential was investigated. Fucose was the main monosaccharide (90 mol%) present in the studied FE, followed by uronic acids, galactose, and xylose that were present at similar values (3.8–2.4 mol%). FE showed a molecular weight of 70 kDa and a sulfate content of around 10%. The expression of cytokines by mouse bone-marrow-derived macrophages (BMDMs) revealed that the addition of FE upregulated the expression of CD206 and IL-10 by about 28 and 22 fold, respectively, in respect to control. This was corroborated in a stimulated pro-inflammatory situation, with the higher expression (60 fold) of iNOS being almost completely reversed by the addition of FE. FE was also capable of reverse LPS-caused inflammation in an in vivo mouse model, including by reducing macrophage activation by LPS from 41% of positive CD11C to 9% upon fucoidan injection. Taken together, the potential of FE as an anti-inflammatory agent was validated, both in vitro and in vivo.
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spelling Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivoFucoidanFucus vesiculosusAnti-inflammatoryMacrophageFucoidan has been reported to present diverse bioactivities, but each extract has specific features from which a particular biological activity, such as immunomodulation, must be confirmed. In this study a commercially available pharmaceutical-grade fucoidan extracted from Fucus vesiculosus, FE, was characterized and its anti-inflammatory potential was investigated. Fucose was the main monosaccharide (90 mol%) present in the studied FE, followed by uronic acids, galactose, and xylose that were present at similar values (3.8–2.4 mol%). FE showed a molecular weight of 70 kDa and a sulfate content of around 10%. The expression of cytokines by mouse bone-marrow-derived macrophages (BMDMs) revealed that the addition of FE upregulated the expression of CD206 and IL-10 by about 28 and 22 fold, respectively, in respect to control. This was corroborated in a stimulated pro-inflammatory situation, with the higher expression (60 fold) of iNOS being almost completely reversed by the addition of FE. FE was also capable of reverse LPS-caused inflammation in an in vivo mouse model, including by reducing macrophage activation by LPS from 41% of positive CD11C to 9% upon fucoidan injection. Taken together, the potential of FE as an anti-inflammatory agent was validated, both in vitro and in vivo.This research received funding from project 0474_BLUEBIOLAB_1_E, financed by the European Regional Development Fund through INTERREG España-Portugal 2014–2020, and project ATLANTIDA (ref. NORTE-01-0145-FEDER-000040) supported by the Northern Portugal Regional Programme Norte 2020, under the Portugal 2020 Partnership Agreement. This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials (UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020) and LAQV-REQUIMTE (UIDB/50006/2020), financed by national funds of the Portuguese Foundation for Science and Technology (FCT)/MCTES. A. S. F. thanks FCT for the individual grant (SFRH/BD/102471/2014). This work was also funded by national funds (OE), through FCT, I.P., within the scope of the framework contract seen in numbers 4, 5 and 6 of article 23 of the Decree Law 57/2016, August 29, changed by Law 57/2017, July 19. The authors are also thankful to the financial support from the Natural Science Foundation of China (grant No. 32000936).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoWang, LingzhiOliveira, Ana Catarina Freitas SalazarLi, QiuFerreira, Andreia S.Nunes, CláudiaCoimbra, Manuel A.Reis, R. L.Martins, AlbinoWang, ChunmingSilva, Tiago H.Feng, Yanxian2023-05-172023-05-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85642engWang, L.; Oliveira, C.; Li, Q.; Ferreira, A.S.; Nunes, C.; Coimbra, M.A.; Reis, R.L.; Martins, A.; Wang, C.; Silva, T.H.; et al. Fucoidan from Fucus vesiculosus Inhibits Inflammatory Response, Both In Vitro and In Vivo. Mar. Drugs 2023, 21, 302. https://doi.org/10.3390/md210503021660-339710.3390/md2105030237233496https://www.mdpi.com/1660-3397/21/5/302info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:18:01Zoai:repositorium.sdum.uminho.pt:1822/85642Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:10:05.528518Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
title Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
spellingShingle Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
Wang, Lingzhi
Fucoidan
Fucus vesiculosus
Anti-inflammatory
Macrophage
title_short Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
title_full Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
title_fullStr Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
title_full_unstemmed Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
title_sort Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro and in vivo
author Wang, Lingzhi
author_facet Wang, Lingzhi
Oliveira, Ana Catarina Freitas Salazar
Li, Qiu
Ferreira, Andreia S.
Nunes, Cláudia
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Wang, Chunming
Silva, Tiago H.
Feng, Yanxian
author_role author
author2 Oliveira, Ana Catarina Freitas Salazar
Li, Qiu
Ferreira, Andreia S.
Nunes, Cláudia
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Wang, Chunming
Silva, Tiago H.
Feng, Yanxian
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Wang, Lingzhi
Oliveira, Ana Catarina Freitas Salazar
Li, Qiu
Ferreira, Andreia S.
Nunes, Cláudia
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Wang, Chunming
Silva, Tiago H.
Feng, Yanxian
dc.subject.por.fl_str_mv Fucoidan
Fucus vesiculosus
Anti-inflammatory
Macrophage
topic Fucoidan
Fucus vesiculosus
Anti-inflammatory
Macrophage
description Fucoidan has been reported to present diverse bioactivities, but each extract has specific features from which a particular biological activity, such as immunomodulation, must be confirmed. In this study a commercially available pharmaceutical-grade fucoidan extracted from Fucus vesiculosus, FE, was characterized and its anti-inflammatory potential was investigated. Fucose was the main monosaccharide (90 mol%) present in the studied FE, followed by uronic acids, galactose, and xylose that were present at similar values (3.8–2.4 mol%). FE showed a molecular weight of 70 kDa and a sulfate content of around 10%. The expression of cytokines by mouse bone-marrow-derived macrophages (BMDMs) revealed that the addition of FE upregulated the expression of CD206 and IL-10 by about 28 and 22 fold, respectively, in respect to control. This was corroborated in a stimulated pro-inflammatory situation, with the higher expression (60 fold) of iNOS being almost completely reversed by the addition of FE. FE was also capable of reverse LPS-caused inflammation in an in vivo mouse model, including by reducing macrophage activation by LPS from 41% of positive CD11C to 9% upon fucoidan injection. Taken together, the potential of FE as an anti-inflammatory agent was validated, both in vitro and in vivo.
publishDate 2023
dc.date.none.fl_str_mv 2023-05-17
2023-05-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/85642
url https://hdl.handle.net/1822/85642
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Wang, L.; Oliveira, C.; Li, Q.; Ferreira, A.S.; Nunes, C.; Coimbra, M.A.; Reis, R.L.; Martins, A.; Wang, C.; Silva, T.H.; et al. Fucoidan from Fucus vesiculosus Inhibits Inflammatory Response, Both In Vitro and In Vivo. Mar. Drugs 2023, 21, 302. https://doi.org/10.3390/md21050302
1660-3397
10.3390/md21050302
37233496
https://www.mdpi.com/1660-3397/21/5/302
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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