Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

Detalhes bibliográficos
Autor(a) principal: Marina Barroso Pereira Pinheiro
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/128679
Resumo: Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery.
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spelling Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung modelBiotecnologia médicaMedical biotechnologyAim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery.2020-05-112020-05-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/10216/128679TID:202616444porMarina Barroso Pereira Pinheiroinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:33:45Zoai:repositorio-aberto.up.pt:10216/128679Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:26:41.772111Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
title Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
spellingShingle Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
Marina Barroso Pereira Pinheiro
Biotecnologia médica
Medical biotechnology
title_short Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
title_full Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
title_fullStr Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
title_full_unstemmed Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
title_sort Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
author Marina Barroso Pereira Pinheiro
author_facet Marina Barroso Pereira Pinheiro
author_role author
dc.contributor.author.fl_str_mv Marina Barroso Pereira Pinheiro
dc.subject.por.fl_str_mv Biotecnologia médica
Medical biotechnology
topic Biotecnologia médica
Medical biotechnology
description Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-11
2020-05-11T00:00:00Z
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dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/128679
TID:202616444
url https://hdl.handle.net/10216/128679
identifier_str_mv TID:202616444
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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