Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6806 |
Resumo: | Microbial cultures able to degrade xenobiotic compounds are the key element for biological treatment of waste effluents and are obtained from enrichment processes. In this study, two common enrichment methods, suspension batch and immobilized continuous, were compared. The main selection factor was the presence of 1,3-dichloro- 2-propanol (1,3-DCP) as the single carbon source. Both methods have successfully enriched microbial consortia able to degrade 1,3-DCP. When tested in batch culture, the degradation rates of 1,3-DCP by the two consortia were different, with the consortia obtained by batch enrichment presenting slightly higher rates. A preliminary morphological and biochemical analysis of the predominant colonial types present in each degrading consortia revealed the presence of different constituting strains. Three bacterial isolates capable of degrading 1,3-DCP as single strains were obtained from the batch enrichments. These strains were classified by 16S rRNA analysis as belonging to the Rhizobiaceae group. Degradation rates of 1,3-DCP were lower when single species were used, reaching 45 mg l−1 d−1, as compared to 74 mg l−1 d−1 of the consortia enriched on the batch method. Mutualistic interactions may explain the better performance of the enriched consortia. |
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Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methodsBatch methodContinuous methodMicrobial enrichmentMicrobial cultures able to degrade xenobiotic compounds are the key element for biological treatment of waste effluents and are obtained from enrichment processes. In this study, two common enrichment methods, suspension batch and immobilized continuous, were compared. The main selection factor was the presence of 1,3-dichloro- 2-propanol (1,3-DCP) as the single carbon source. Both methods have successfully enriched microbial consortia able to degrade 1,3-DCP. When tested in batch culture, the degradation rates of 1,3-DCP by the two consortia were different, with the consortia obtained by batch enrichment presenting slightly higher rates. A preliminary morphological and biochemical analysis of the predominant colonial types present in each degrading consortia revealed the presence of different constituting strains. Three bacterial isolates capable of degrading 1,3-DCP as single strains were obtained from the batch enrichments. These strains were classified by 16S rRNA analysis as belonging to the Rhizobiaceae group. Degradation rates of 1,3-DCP were lower when single species were used, reaching 45 mg l−1 d−1, as compared to 74 mg l−1 d−1 of the consortia enriched on the batch method. Mutualistic interactions may explain the better performance of the enriched consortia.Kluwer Academic PublishersVeritati - Repositório Institucional da Universidade Católica PortuguesaBastos, FilipeBessa, JoséPacheco, Catarina C.Marco, Paolo DeCastro, Paula M.L.2011-10-22T11:59:59Z20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6806engBASTOS, Filipe...[et al] - Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods. Biodegradation. ISSN 0923-9820. Vol. 13:(2002), p. 211–22010.1023/a:1020834603785info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:31Zoai:repositorio.ucp.pt:10400.14/6806Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:47.201246Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
title |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
spellingShingle |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods Bastos, Filipe Batch method Continuous method Microbial enrichment |
title_short |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
title_full |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
title_fullStr |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
title_full_unstemmed |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
title_sort |
Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods |
author |
Bastos, Filipe |
author_facet |
Bastos, Filipe Bessa, José Pacheco, Catarina C. Marco, Paolo De Castro, Paula M.L. |
author_role |
author |
author2 |
Bessa, José Pacheco, Catarina C. Marco, Paolo De Castro, Paula M.L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Bastos, Filipe Bessa, José Pacheco, Catarina C. Marco, Paolo De Castro, Paula M.L. |
dc.subject.por.fl_str_mv |
Batch method Continuous method Microbial enrichment |
topic |
Batch method Continuous method Microbial enrichment |
description |
Microbial cultures able to degrade xenobiotic compounds are the key element for biological treatment of waste effluents and are obtained from enrichment processes. In this study, two common enrichment methods, suspension batch and immobilized continuous, were compared. The main selection factor was the presence of 1,3-dichloro- 2-propanol (1,3-DCP) as the single carbon source. Both methods have successfully enriched microbial consortia able to degrade 1,3-DCP. When tested in batch culture, the degradation rates of 1,3-DCP by the two consortia were different, with the consortia obtained by batch enrichment presenting slightly higher rates. A preliminary morphological and biochemical analysis of the predominant colonial types present in each degrading consortia revealed the presence of different constituting strains. Three bacterial isolates capable of degrading 1,3-DCP as single strains were obtained from the batch enrichments. These strains were classified by 16S rRNA analysis as belonging to the Rhizobiaceae group. Degradation rates of 1,3-DCP were lower when single species were used, reaching 45 mg l−1 d−1, as compared to 74 mg l−1 d−1 of the consortia enriched on the batch method. Mutualistic interactions may explain the better performance of the enriched consortia. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 2002-01-01T00:00:00Z 2011-10-22T11:59:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6806 |
url |
http://hdl.handle.net/10400.14/6806 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BASTOS, Filipe...[et al] - Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods. Biodegradation. ISSN 0923-9820. Vol. 13:(2002), p. 211–220 10.1023/a:1020834603785 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Kluwer Academic Publishers |
publisher.none.fl_str_mv |
Kluwer Academic Publishers |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131723453169664 |