An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency

Detalhes bibliográficos
Autor(a) principal: Costa, Filomena
Data de Publicação: 2014
Outros Autores: Quintelas, C., Tavares, M. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31749
Resumo: Abstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process.
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spelling An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiencyBiodegradation kineticsDegradationDiethylketoneGrowth kineticsMetabolic pathwayStreptococcus equisimilisScience & TechnologyAbstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process.The authors would like to gratefully acknowledge the financial support of this project by the Fundacao para a Ciencia e Tecnologia, Ministerio da Ciencia e Tecnologia, Portugal and co-funding by FSE (programme QREN - POPH) - PEst-OE/EQB/LA0023/2013. The authors thank the Project "BioEnv-Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER. Cristina Quintelas thanks Cristina Quintelas thanks FCT for a Post-Doc (SFRH/BPD/32113/2006) and Filomena Costa thanks FCT for a PhD grant (SFRH/BD/77666/2011).Elsevier B.V.Universidade do MinhoCosta, FilomenaQuintelas, C.Tavares, M. T.2014-092014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31749eng0925-857410.1016/j.ecoleng.2014.05.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:09Zoai:repositorium.sdum.uminho.pt:1822/31749Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:11.843528Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
title An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
spellingShingle An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
Costa, Filomena
Biodegradation kinetics
Degradation
Diethylketone
Growth kinetics
Metabolic pathway
Streptococcus equisimilis
Science & Technology
title_short An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
title_full An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
title_fullStr An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
title_full_unstemmed An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
title_sort An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
author Costa, Filomena
author_facet Costa, Filomena
Quintelas, C.
Tavares, M. T.
author_role author
author2 Quintelas, C.
Tavares, M. T.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, Filomena
Quintelas, C.
Tavares, M. T.
dc.subject.por.fl_str_mv Biodegradation kinetics
Degradation
Diethylketone
Growth kinetics
Metabolic pathway
Streptococcus equisimilis
Science & Technology
topic Biodegradation kinetics
Degradation
Diethylketone
Growth kinetics
Metabolic pathway
Streptococcus equisimilis
Science & Technology
description Abstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
2014-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31749
url http://hdl.handle.net/1822/31749
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0925-8574
10.1016/j.ecoleng.2014.05.009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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