An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31749 |
Resumo: | Abstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process. |
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An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiencyBiodegradation kineticsDegradationDiethylketoneGrowth kineticsMetabolic pathwayStreptococcus equisimilisScience & TechnologyAbstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process.The authors would like to gratefully acknowledge the financial support of this project by the Fundacao para a Ciencia e Tecnologia, Ministerio da Ciencia e Tecnologia, Portugal and co-funding by FSE (programme QREN - POPH) - PEst-OE/EQB/LA0023/2013. The authors thank the Project "BioEnv-Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER. Cristina Quintelas thanks Cristina Quintelas thanks FCT for a Post-Doc (SFRH/BPD/32113/2006) and Filomena Costa thanks FCT for a PhD grant (SFRH/BD/77666/2011).Elsevier B.V.Universidade do MinhoCosta, FilomenaQuintelas, C.Tavares, M. T.2014-092014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31749eng0925-857410.1016/j.ecoleng.2014.05.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:09Zoai:repositorium.sdum.uminho.pt:1822/31749Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:11.843528Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
title |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
spellingShingle |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency Costa, Filomena Biodegradation kinetics Degradation Diethylketone Growth kinetics Metabolic pathway Streptococcus equisimilis Science & Technology |
title_short |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
title_full |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
title_fullStr |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
title_full_unstemmed |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
title_sort |
An approach to the metabolic degradation of diethylketone (DEK) by Streptococcus equisimilis : effect of DEK on the growth, biodegradation kinetics and efficiency |
author |
Costa, Filomena |
author_facet |
Costa, Filomena Quintelas, C. Tavares, M. T. |
author_role |
author |
author2 |
Quintelas, C. Tavares, M. T. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Costa, Filomena Quintelas, C. Tavares, M. T. |
dc.subject.por.fl_str_mv |
Biodegradation kinetics Degradation Diethylketone Growth kinetics Metabolic pathway Streptococcus equisimilis Science & Technology |
topic |
Biodegradation kinetics Degradation Diethylketone Growth kinetics Metabolic pathway Streptococcus equisimilis Science & Technology |
description |
Abstract The degradation of diethylketone by Streptococcus equisimilis was evaluated. The toxicity of diethylketone was assessed evaluating the growth of the bacteria for the range of diethylketone concentration between 0 and 6.4 g L−1. The maximum specific growth rate achieved is 0.555 h−1 at 3.2 g L−1 of initial diethylketone concentration, followed by a slight decrease, suggesting that higher concentrations of diethylketone negatively affect the growth. The biodegradation efficiency (%) obtained was approximately 95%, for all the initial concentrations tested. The kinetic parameters were estimated using Monod, Powell, Haldane and Loung models. The experimental data is well fitted by the Loung and Haldane models, R2 = 0.98 for both, as compared to Monod model (R2 = 0.79) and Powell model (R2 = 0.72). Once the previous intrinsic functions were established, a set of assays was also performed to evaluate the biodegradation of diethylketone using concentrated biomass for initial diethylketone concentrations ranging from 0.8 to 3.9 g L−1 in a bioreactor operating in batch mode with recirculation. These concentrated biomass assays aimed the optimization of operational conditions. The removal percentages obtained were approximately 100%, for all the initial ketone concentrations tested and the biodegradation rate followed the pseudo-second order kinetics. The mechanism involved in the degradation of diethylketone by this microorganism is not completely understood, but an approach to the metabolic degradation pathway was established by the identification of the metabolites involved on the process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 2014-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31749 |
url |
http://hdl.handle.net/1822/31749 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0925-8574 10.1016/j.ecoleng.2014.05.009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132464187179008 |