A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/4184 |
Resumo: | The Supplementary Material for this article can be found online at http://journal.frontiersin.org/article/10.3389/fimmu.2016.00162 |
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A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis ClearanceChlamydia TrachomatisVaccineDNA VaccinesAdenovirus-vector VaccinesConsensusMosaicPrime-boost RegimensInfecções Sexualmente TransmissíveisThe Supplementary Material for this article can be found online at http://journal.frontiersin.org/article/10.3389/fimmu.2016.00162BACKGROUND: A vaccine for Chlamydia trachomatis is of urgent medical need. We explored bioinformatic approaches to generate an immunogen against C. trachomatis that would induce cross-serovar T-cell responses as (i) CD4(+) T cells have been shown in animal models and human studies to be important in chlamydial protection and (ii) antibody responses may be restrictive and serovar specific. METHODS: A consensus antigen based on over 1,500 major outer membrane protein (MOMP) sequences provided high epitope coverage against the most prevalent C. trachomatis strains in silico. Having designed the T-cell immunogen, we assessed it for immunogenicity in prime-boost regimens. This consensus MOMP transgene was delivered using plasmid DNA, Human Adenovirus 5 (HuAd5) or modified vaccinia Ankara (MVA) vectors with or without MF59(®) adjuvanted recombinant MOMP protein. RESULTS: Different regimens induced distinct immune profiles. The DNA-HuAd5-MVA-Protein vaccine regimen induced a cellular response with a Th1-biased serum antibody response, alongside high serum and vaginal MOMP-specific antibodies. This regimen significantly enhanced clearance against intravaginal C. trachomatis serovar D infection in both BALB/c and B6C3F1 mouse strains. This enhanced clearance was shown to be CD4(+) T-cell dependent. Future studies will need to confirm the specificity and precise mechanisms of protection. CONCLUSION: A C. trachomatis vaccine needs to induce a robust cellular response with broad cross-serovar coverage and a heterologous prime-boost regimen may be an approach to achieve this.AB was funded by the Wellcome Trust. RS was supported by the European Community’s European 7th Framework Program ADITEC (HEALTH-F4-2011-18 280873).Repositório Científico do Instituto Nacional de SaúdeBadamchi-Zadeh, AlexanderMcKay, Paul F.Korber, Bette T.Barinaga, GuillermoWalters, Adam A.Nunes, AlexandraGomes, João PauloFollmann, FrankTregoning, John S.Shattock, Robin J.2017-02-15T16:02:11Z2016-04-282016-04-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4184engFront Immunol. 2016 Apr 28;7:162. doi: 10.3389/fimmu.2016.00162. eCollection 20161664-322410.3389/fimmu.2016.00162info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:20Zoai:repositorio.insa.pt:10400.18/4184Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:13.304292Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| title |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| spellingShingle |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance Badamchi-Zadeh, Alexander Chlamydia Trachomatis Vaccine DNA Vaccines Adenovirus-vector Vaccines Consensus Mosaic Prime-boost Regimens Infecções Sexualmente Transmissíveis |
| title_short |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| title_full |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| title_fullStr |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| title_full_unstemmed |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| title_sort |
A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance |
| author |
Badamchi-Zadeh, Alexander |
| author_facet |
Badamchi-Zadeh, Alexander McKay, Paul F. Korber, Bette T. Barinaga, Guillermo Walters, Adam A. Nunes, Alexandra Gomes, João Paulo Follmann, Frank Tregoning, John S. Shattock, Robin J. |
| author_role |
author |
| author2 |
McKay, Paul F. Korber, Bette T. Barinaga, Guillermo Walters, Adam A. Nunes, Alexandra Gomes, João Paulo Follmann, Frank Tregoning, John S. Shattock, Robin J. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Badamchi-Zadeh, Alexander McKay, Paul F. Korber, Bette T. Barinaga, Guillermo Walters, Adam A. Nunes, Alexandra Gomes, João Paulo Follmann, Frank Tregoning, John S. Shattock, Robin J. |
| dc.subject.por.fl_str_mv |
Chlamydia Trachomatis Vaccine DNA Vaccines Adenovirus-vector Vaccines Consensus Mosaic Prime-boost Regimens Infecções Sexualmente Transmissíveis |
| topic |
Chlamydia Trachomatis Vaccine DNA Vaccines Adenovirus-vector Vaccines Consensus Mosaic Prime-boost Regimens Infecções Sexualmente Transmissíveis |
| description |
The Supplementary Material for this article can be found online at http://journal.frontiersin.org/article/10.3389/fimmu.2016.00162 |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-04-28 2016-04-28T00:00:00Z 2017-02-15T16:02:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4184 |
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http://hdl.handle.net/10400.18/4184 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Front Immunol. 2016 Apr 28;7:162. doi: 10.3389/fimmu.2016.00162. eCollection 2016 1664-3224 10.3389/fimmu.2016.00162 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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RCAAP |
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